Enhancement of anchorage‐independent growth of a human adrenal carcinoma cell line by endogenously produced basic fibroblast growth factor

Abstract

We have previously observed that a human adrenal carcinoma cell line (SW-13) produces autocrine growth factors that enhance the ability of these cells to clone in soft agar. We now show that a basic FGF-like protein is found in SW-13 lysates and stimulates SW-13 colony growth. Basic FGF (bFGF) was identified in SW-13 lysates by both its chromatographic behavior on heparin-Sepharose and its reactivity with an antibody specific for bFGF. Incubation of lysates with antibodies specific for bFGF abolished their ability to support anchorage-independent growth. Northern analysis demonstrated that the cells produced multiple bFGF mRNA transcripts. SW-13 cells also expressed bFGF cell-surface receptors. These data suggest that SW-13 cells produce and respond to a factor structurally and functionally related to bFGF. Such findings support recent evidence indicating that FGF secreted by human tumor cell lines plays a role in the maintenance of the transformed phenotype.

Adrenocortical carcinoma is characterized by a high frequency of chromosomal gains and high‐level amplifications

“…Fibroblast growth factors 12 and 14 are localized within two of the amplified regions, namely, 3q28qter and 13q34, respectively. It has previously been shown that the adrenocortical carcinoma cell line SW13 produces autocrine fibroblast growth factors mediating anchorage-independent growth (Corin et al, 1990). CGH of SW13 revealed amplification of the chromosomal segment harboring FGF12.…”

Adrenocortical carcinoma is characterized by a high frequency of chromosomal gains and high-level amplifications

“…Fibroblast growth factors 12 and 14 are localized within two of the amplified regions, namely, 3q28–qter and 13q34, respectively. It has previously been shown that the adrenocortical carcinoma cell line SW13 produces autocrine fibroblast growth factors mediating anchorage‐independent growth (Corin et al, 1990). CGH of SW13 revealed amplification of the chromosomal segment harboring FGF12 .…”

Análise comparativa dos transcritomas do córtex adrenal normal e adenocarcinoma do córtex adrenal

“…Nestes segmentos encontram-se os loci dos fatores de crescimento de fibroblasto (FGF) 12 e 14, respectivamente [34] . As células SW13 produzem autocrinamente FGFs que conferem o crescimento independente de ancoragem (capacidade de proliferar, formar colônias em ágar) [37] . Especulase que a amplificação do 3q28-qter seja responsável pela produção autócrina do FGF12 por essas células e, conseqüentemente, pelo crescimento independente de ancoragem [34] .…”