Enhancement of antibody production by the addition of Coenzyme-Q10

Abstract

Recently, there has been a growing demand for therapeutic monoclonal antibodies (MAbs) on the global market. Because therapeutic MAbs are more expensive than low-molecular-weight drugs, there have been strong demands to lower their production costs. Therefore, efficient methods to minimize the cost of goods are currently active areas of research. We have screened several enhancers of specific MAb production rate (SPR) using a YB2/0 cell line and found that coenzyme-Q(10) (CoQ(10)) is a promising enhancer candidate. CoQ(10) is well known as a strong antioxidant in the respiratory chain and is used for healthcare and other applications. Because CoQ(10) is negligibly water soluble, most studies are limited by low concentrations. We added CoQ(10) to a culture medium as dispersed nanoparticles at several concentrations (Q-Media) and conducted a fed-batch culture. Although the Q-Media had no effect on cumulative viable cell density, it enhanced SPR by 29%. In addition, the Q-Media had no effect on the binding or cytotoxic activity of MAbs. Q-Media also enhanced SPR with CHO and NS0 cell lines by 30%. These observations suggest that CoQ(10) serves as a powerful aid in the production of MAbs by enhancing SPR without changing the characteristics of cell growth, or adversely affecting the quality or biological activity of MAbs.

A Pilot Study of the Photoprotective Effects of Strawberry-Based Cosmetic Formulations on Human Dermal Fibroblasts

“…For these reasons, we used Sanomit™ Q 10 for CoQ 10ox and Quinomit™ Q 10 for CoQ 10red , which are dispersions of nanoparticles (diameter < 50 nm) of CoQ 10 in water and are useful for preparing stable liquid-liquid dispersions of various CoQ 10 concentrations. These two products are absorbed much better and faster compared to ordinary Q 10 preparations, as previously reported [ 22 , 23 ].…”

Advantages of AlaGln as an additive to cell culture medium: use with anti-CD20 chimeric antibody-producing POTELLIGENT™ CHO cell lines

“…Usually, these tend to be a mixture of several components or materials that was synthesized de novo. Examples of such supplements that have been adopted for use in culture media include sericin (Terada et al 2005) and coenzyme Q10 (Konno et al 2011;Sun et al 1995). Glutamine (Gln) is a key component of culture medium because it is an essential amino acid, especially for those mammalian cells that are deficient in the gene for glutamine (Gln) synthesis.…”

Fucose content of monoclonal antibodies can be controlled by culture medium osmolality for high antibody-dependent cellular cytotoxicity

“…This is because YB2/0 cells produce MAbs with a lower fucose content than those by CHO or NS0 cells. Although YB2/0 appears to be a good candidate for the large-scale production of high activity MAbs, there have been limited reports about the bio-process development in these cells to be employed with confidence (Arathoon and Birch 1986;Keen 1995;Konno et al 2011;Rhodes and Birch 1988;Teylaert et al 2011). In addition, we have observed that the defucosylation levels (deFuc%) of oligosaccharides in MAbs produced by YB2/0 vary according to cell culture conditions (unpublished observations).…”