Enhancement of antimicrobial activity by liposomal oleic acid-loaded antibiotics for the treatment of multidrug-resistant Pseudomonas aeruginosa

Abstract

In this study, we examined the efficacy of liposomal oleic acid-based antibiotic formulations on 32 strains of multidrug-resistant Pseudomonas aeruginosa (MDRPa). The average size of liposomes were 93.12 ± 2.3 nm holding a negative zeta potential at -57.3 ± 0.89. Liposomal antibiotic formulations were tested against 32 MDRPa strains isolated from burn wounds and urine samples, which exhibited an MIC of ≤8 μg/mL, whereas MIC of free antibiotics ranged from 32 to >1024 μg/mL. The results clearly indicate that the liposomes composed of naturally occurring oleic acid, could be used therapeutically either alone or in combination with antibiotics to effectively treat P. aeruginosa infections.

Novel pyochelin-based PEGylated liposomes for enhanced delivery of antibiotics against resistant clinical isolates of Pseudomonas aeruginosa

“…Based on our results from killing curve, liposomal pyochelin formulations encapsulated with the examined antibiotics demonstrated enhanced eradication of laboratory strain and clinical strains. Indeed, these observations have shown significantly lower MICs than liposomal antibiotics against P. aeruginosa strains [12].…”

“…Furthermore, it has been proved that the use of oleic acid, a monounsaturated fatty acid, not only increase the stability of liposomes but also improve the cytoplasmic delivery of encapsulated drugs [11]. Recently, our group has reported the efficacy of liposomes composed of oleic acid exhibited potent bactericidal effect if used either alone or in combination with antibiotics against MDRPa [12].…”

Emerging therapies against infections with Pseudomonas aeruginosa

“…Anti-pseudomonal drugs such as ciprofloxacin 103– 105 , meropenem 106 , tobramycin 107, 108 , gentamicin 109 , or amikacin 110 were encapsulated into liposomes or loaded into nanoparticles. The drug delivery systems were diverse in chemical nature and include anionic liposomes 105, 106, 109 , poly(lactic-co-glycolic) acid nanoparticles 110, 111 , water-soluble chitosan oligosaccharide conjugates 112 , oil-in-water cross-linked polymeric nanocomposites 113 , graphen-oxide conjugates 107 , or solid lipid nanoparticles 114 , to name just a few. Alternatively, dry powders 103, 104, 108, 115 or hydrogels 116– 119 were formulated or wound dressings were coated with a topical antimicrobial such as silver oxynitrate 120 .…”

Prospects of Nanostructure Materials and Their Composites as Antimicrobial Agents

“…In last few years, antimicrobial drugs encapsulated in ONP systems have appeared as path-breaking and promising alternatives that have not only increased therapeutic index but also reduced detrimental side effects of the drug (Yang et al, 2009 ; Nath and Banerjee, 2013 ). Currently, liposome is one of the most commonly used antimicrobial drug delivery system because it can mimic the microbial cell membrane and easily fuse with the pathogenic microbe (Pushparaj Selvadoss et al, 2017 ). Owing to the unhindered fusion of microbial cell membrane and liposome, cargos (drugs) easily get released inside the microbial cell and eventually result in its death (Walsh et al, 2001 ; Yang et al, 2009 ).…”