Enhancement of antimycobacterial Th1-cell responses by a Mycobacterium bovis BCG prime-protein boost vaccination strategy

Lu, Miao; Xia, Zhi; Bao, Linlin

doi:10.1016/j.cellimm.2013.10.001

Cited by 7 publications

(9 citation statements)

References 28 publications

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“…Previously, much effort has been made by our group and others in the development of safe and effective boost vaccine candidates after the BCG priming vaccination, such as giving a BCG prime followed by boosting with a viral vector, recombinant protein, or DNA vaccine [7][8][9]. These studies revealed that BCG prime-boost approach could activate T cell to evoke strong immune responses including both CD4+ T cell and CD8+ T cell immune responses [10].…”

Section: Introductionmentioning

confidence: 99%

The <italic>Mycobacterium bovis</italic> BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

Gu¹,

Chen²,

Mi³

et al. 2016

ABBS

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Tuberculosis remains a major global health problem and effective vaccines are urgently needed. In this study, we used the combined DNA-and protein-based vaccines of immunodominant antigen Rv0577 to boost BCG and evaluated their immunogenicity in BALB/c mice. Our data suggest that the booster vaccine may substantially enhance the immunogenicity of BCG and strengthen both CD4+ T cell-mediated Th1 and CD8+ T cell-mediated cytolytic responses. Compared with the protein-based vaccine, the DNA-based vaccine can induce more durable Th1 immune response, characterized by high levels of antibody response, proliferation response, percentages of CD4+/CD8+ and cytokine secretion in antigen-stimulated splenocyte cultures. In conclusion, we for the first time, developed a protein-and plasmid DNA-based booster vaccine based on Rv0577. Our findings suggest that antigen Rv0577-based DNA vaccine is immunogenic and can efficiently boost BCG, which could be helpful in the design of an efficient vaccination strategy against TB.

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Section: Introductionmentioning

confidence: 99%

The <italic>Mycobacterium bovis</italic> BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

Gu¹,

Chen²,

Mi³

et al. 2016

ABBS

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“…Heterologous prime/boost vaccination strategies employing recombinant bacteria, viruses, proteins, and naked DNA have been shown to elicit stronger and more diverse cellular immune responses than BCG vaccine alone [5–7, 22]. In humans, DNA vaccines alone have not provided satisfactory results, whereas DNA vaccines produced better outcomes when immunized as a prime-boost strategy [29, 30].…”

Section: Discussionmentioning

confidence: 99%

“…The method was described as previous [22, 26]. Each sera sample was tested in three replicates, and the results are expressed as mean ± standard errors.…”

Section: Methodsmentioning

confidence: 99%

“…The proliferation of lymphocyte were tested by MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide]. The method was described as previous [22, 27]. The results are expressed as the value of stimulation index (SI).…”

Section: Methodsmentioning

confidence: 99%

“…In our research, we choose two BCG substrains (BCG-Pasteur1173 and BCG-China) which are different in two deletions called RD14 and N-RD18 [19, 20], which are present in BCG-China, but absent in BCG-Pasteur1173. We notice two genes (Rv1769 and Rv1772) in RD14 deletion, which have been studied superficially, and some research has indicated that Rv1769 and Rv1772 should be considered for potential subunit vaccines [21, 22]. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

AMycobacterium bovisBCG-Naked DNA Prime-Boost Vaccination Strategy Induced CD4⁺and CD8⁺T-Cell Response againstMycobacterium tuberculosisImmunogens

Xia

Bao

2014

Journal of Immunology Research

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Mycobacterium tuberculosis infection is still a major global public health problem. Presently the only tuberculosis (TB) vaccine available is Bacille Calmette-Guérin (BCG), although it fails to adequately protect against pulmonary TB in adults. To solve this problem, the development of a new effective vaccine is urgently desired. BCG-prime DNA-booster vaccinations strategy has been shown to induce greater protection against tuberculosis (TB) than BCG alone. Some studies have demonstrated that the two genes (Rv1769 and Rv1772) are excellent T-cell antigens and could induce T-cell immune responses. In this research, we built BCG-C or BCG-P prime-recombination plasmid PcDNA3.1-Rv1769 or PcDNA3.1-Rv1772 boost vaccinations strategy to immunize BALB/c mice and evaluated its immunogenicity. The data suggests that the BCG-C+3.1-72 strategy could elicit the most long-lasting and strongest Th1-type cellular immune responses and the BCG-C+3.1-69 strategy could induce the high level CD8+ T-cell response at certain time points. These findings support the ideas that the prime-boost strategy as a combination of vaccines may be better than a single vaccine for protection against tuberculosis.

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Simultaneous priming with DNA encoding Sm-p80 and boosting with Sm-p80 protein confers protection against challenge infection with Schistosoma mansoni in mice

Zhang

Karmakar

et al. 2014

Parasitol Res

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Prophylactic efficacy of Sm-p80 was tested in the mouse model using DNA priming and boosting with protein approach. However, the novelty of the approach utilized in this study is that both the DNA priming and protein boosting was performed on a single day and no further vaccine inoculations were given to mice; the animals were challenged 1 month after the initial vaccine administration. Using this approach, significant reduction in worm burden (33 to 57 %) and marked decrease in egg retention in tissues (34 to 66%) was observed. Robust antibody titers and upregulation of cytokines (IL-1α/β, IL-12α, and IFN-γ) appears to correlate with the protection. This approach of administering vaccine on a single day could be greatly helpful in the field setting because it will eliminate the compliance issues that may arise with multiple boosters that may be required for optimal efficacy for some vaccines.

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Enhancement of antimycobacterial Th1-cell responses by a Mycobacterium bovis BCG prime-protein boost vaccination strategy

Cited by 7 publications

References 28 publications

The <italic>Mycobacterium bovis</italic> BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

The <italic>Mycobacterium bovis</italic> BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

AMycobacterium bovisBCG-Naked DNA Prime-Boost Vaccination Strategy Induced CD4⁺and CD8⁺T-Cell Response againstMycobacterium tuberculosisImmunogens

Simultaneous priming with DNA encoding Sm-p80 and boosting with Sm-p80 protein confers protection against challenge infection with Schistosoma mansoni in mice

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Enhancement of antimycobacterial Th1-cell responses by a Mycobacterium bovis BCG prime-protein boost vaccination strategy

Cited by 7 publications

References 28 publications

The &lt;italic&gt;Mycobacterium bovis&lt;/italic&gt; BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

The &lt;italic&gt;Mycobacterium bovis&lt;/italic&gt; BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

AMycobacterium bovisBCG-Naked DNA Prime-Boost Vaccination Strategy Induced CD4+and CD8+T-Cell Response againstMycobacterium tuberculosisImmunogens

Simultaneous priming with DNA encoding Sm-p80 and boosting with Sm-p80 protein confers protection against challenge infection with Schistosoma mansoni in mice

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The <italic>Mycobacterium bovis</italic> BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

The <italic>Mycobacterium bovis</italic> BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice

AMycobacterium bovisBCG-Naked DNA Prime-Boost Vaccination Strategy Induced CD4⁺and CD8⁺T-Cell Response againstMycobacterium tuberculosisImmunogens