With the rapid growth in global population and standards of living, improving food production and quality are the greatest challenges in agriculture. The application of biostimulant derived from moringa leaf extract (MLE) has attracted a great deal of interest to support these efforts in a sustainable approach. A field study was conducted using a randomized complete block design with four replications. The effect of individual and combined application of MLE through seed priming (seed soaking) and foliar spray at different rates (1:30, 1:20, and 1:10, v/v) on growth, physiological, yield, and quality traits of green chili pepper were investigated, which was the aim of this study. In general, the responses generated by the combined MLE methods were more pronounced compared to their single applications. Among all treatments, MLE priming plus foliar spray at 1:30 was effective in improving most traits observed, including chlorophyll fluorescence (6.49%), stomatal conductance (57.19%), plant height (30.57%), leaf number (88.89%), leaf area index (116.67%), fruit weight per plant (46.27%), average fruit weight (39.62%), length (9.89%), diameter (29.65%), firmness (27.77%), and vitamin C content (29.07%) of fruit. Therefore, it is regarded as an appropriate treatment to maximize the potential use of MLE in green chili.