Enhancement of autofluorescence of the brown-rot fungus Piptoporus betulinus by metal ions

Žižka, Z; Větrovský, Tomáš; Gabriel, Jiřı́

doi:10.1007/s12223-010-0101-y

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…The host range of the fungus is restricted exclusively to birch species, e.g. Betula pendula Roth., B. pubescens Ehrh., B. papyrifera Marsh., and B. obscura Kotula (Schwarze 1993; Žižka et al 2010). …”

Section: Taxonomy and Characteristicsmentioning

confidence: 99%

Fomitopsis betulina (formerly Piptoporus betulinus): the Iceman’s polypore fungus with modern biotechnological potential

Pleszczyńska

Lemieszek

Siwulski

et al. 2017

World J Microbiol Biotechnol

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Higher Basidiomycota have been used in natural medicine throughout the world for centuries. One of such fungi is Fomitopsis betulina (formerly Piptoporus betulinus), which causes brown rot of birch wood. Annual white to brownish fruiting bodies of the species can be found on trees in the northern hemisphere but F. betulina can also be cultured as a mycelium and fruiting body. The fungus has a long tradition of being applied in folk medicine as an antimicrobial, anticancer, and anti-inflammatory agent. Probably due to the curative properties, pieces of its fruiting body were carried by Ötzi the Iceman. Modern research confirms the health-promoting benefits of F. betulina. Pharmacological studies have provided evidence supporting the antibacterial, anti-parasitic, antiviral, anti-inflammatory, anticancer, neuroprotective, and immunomodulating activities of F. betulina preparations. Biologically active compounds such as triterpenoids have been isolated. The mushroom is also a reservoir of valuable enzymes and other substances such as cell wall (1→3)-α-d-glucan which can be used for induction of microbial enzymes degrading cariogenic dental biofilm. In conclusion, F. betulina can be considered as a promising source for the development of new products for healthcare and other biotechnological uses.

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Section: Taxonomy and Characteristicsmentioning

confidence: 99%

Fomitopsis betulina (formerly Piptoporus betulinus): the Iceman’s polypore fungus with modern biotechnological potential

Pleszczyńska

Lemieszek

Siwulski

et al. 2017

World J Microbiol Biotechnol

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“…AF is closely associated with compounds of the cell wall, in which chitin may have a significant role. Adding metal ions or altering the pH also affects the generation of AF ( 38 , 39 ). Therefore, protoplast preparation and culture adjustment was performed to acquire the least AF.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of an anti-oxidative stress-associated mutant of Aspergillus fumigatus transformed by Agrobacterium tumefaciens

Fan

Guo

et al. 2016

Molecular Medicine Reports

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Aspergillus fumigatus is one of the most common opportunistic pathogenic fungi, surviving in various environmental conditions. Maintenance of the redox homeostasis of the fungus relies upon the well-organized regulation between reactive oxygen species generated by immune cells or its own organelles, and the activated anti-oxidative stress mechanism. To investigate such a mechanism, the present study obtained a number of randomly-inserted mutants of A. fumigatus, mediated by Agrobacterium tumefaciens. In addition, a high throughput hydrogen peroxide screening system was established to examine ~1,000 mutants. A total of 100 mutants exhibited changes in hydrogen peroxide sensitivity, among which a significant increase in sensitivity was observed in the AFM2658 mutant. Further investigations of the mutant were also performed, in which the sequence of this mutant was characterized using thermal asymmetric interlaced-polymerase chain reaction. This revealed that the insertion site was located on chromosome 2 afu1_92, and the 96 bp sequence was knocked out, which partially comprised a sequence localized between the integral membrane protein coding region and the helix-loop-helix transcription factor coding region. A decrease in the levels of anti-oxidative stress-associated mRNAs were observed, and an increase in reactive oxygen species were detected using fluorescence. The results of the present study demonstrated that this sequence may have a protective role in A. fumigatus in the presence of oxidative stress.

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“…By contrast, autofluorescence originating from endogenous fluorophores in organisms is a natural indicator, and these limited autofluorescent biomolecules have a fixed distribution. For example, combined with label-free fluorescence microscopy and spectrometer, detecting the distribution of intracellular components and monitoring the metabolic status of mycelium were realized in corn pathogenic fungi, arbuscular mycorrhizal fungi, brown-rot fungus, and bacteria [ 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of conidial autofluorescence in powdery mildew

Liu

et al. 2022

Heliyon

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Enhancement of autofluorescence of the brown-rot fungus Piptoporus betulinus by metal ions

Cited by 6 publications

References 16 publications

Fomitopsis betulina (formerly Piptoporus betulinus): the Iceman’s polypore fungus with modern biotechnological potential

Fomitopsis betulina (formerly Piptoporus betulinus): the Iceman’s polypore fungus with modern biotechnological potential

Identification and characterization of an anti-oxidative stress-associated mutant of Aspergillus fumigatus transformed by Agrobacterium tumefaciens

Characterization of conidial autofluorescence in powdery mildew

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