Enhancement of baculovirus infection in Spodoptera exigua (Lepidoptera: Noctuidae) larvae with Autographa californica nucleopolyhedrovirus or Nicotiana tabacum engineered with a granulovirus enhancin gene.

Hayakawa, Tohru; Shimojo, Ei Ichi; Mori, Masashi; Kaido, Masanori; Furusawa, Iwao; Miyata, Seiji; Sano, Yoshitaka; Matsumoto, Tsuguo; Hashimoto, Yoshifumi; Granados, Robert R.

doi:10.1303/aez.2000.163

Cited by 31 publications

(26 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, the enhancin gene was introduced into tobacco plant and the transgenic tobacco showed insecticidal activity to Spodoptera exigua, resulting in stunted larval growth, abnormal pupation, and increased larval susceptibility to baculovirus infection (Hashimoto et al, 1994;Hayakawa et al, 2000). These recent studies suggest the feasibility of targeting the PM using a PM specific proteolytic enzyme from a baculovirus.…”

Section: Pm Targeting For Insect Controlmentioning

confidence: 99%

Molecular structure of the peritrophic membrane (PM): Identification of potential PM target sites for insect control

Wang

Granados

2001

Arch Insect Biochem Physiol

Self Cite

198

163

View full text Add to dashboard Cite

Peritrophic membranes (PMs) are an invertebrate-unique structure that lines the digestive tract, playing important roles in facilitating food digestion and providing protection to the gut epithelium. The importance of PMs in insects has been recognized ever since its presence was identified 200 years ago. In the last 5 years, significant progress towards understanding the PM molecular structure and the mechanism for PM formation has been made. Recent studies on Type 1 PMs from lepidopteran larvae have suggested a model for the PM molecular structure and formation. The important physiological functions of the PM suggest that PMs can be a significant structural target for insect control and the current understanding of the structure of lepidopteran larval PMs has provided us with potential opportunities for targeting the PM by various mechanisms.

show abstract

Section: Pm Targeting For Insect Controlmentioning

confidence: 99%

Molecular structure of the peritrophic membrane (PM): Identification of potential PM target sites for insect control

Wang

Granados

2001

Arch Insect Biochem Physiol

Self Cite

198

163

View full text Add to dashboard Cite

show abstract

“…SeNPV OBs were prepared from S. exigua larvae infected with the virus as described by Hayakawa et al (2000).…”

Section: Insect Bioassay Of Nuclear Polyhedrosis Virus Infection Enhamentioning

confidence: 99%

“…Transgenic tobacco plants supplemented with the Plant Biotechnology 23, 55-61 (2006) Expression of an enhancin gene from the Trichoplusia ni granulosis virus confers resistance to lepidopterous insect pests to rice TnGV enhancin gene enhanced infection of the Spodoptera exigua nucleopolyhedrovirus (SeNPV) in the larvae of a lepidopteran insect, S. exigua, and inhibited the growth of T. ni (Hayakawa et al 2000;Cao et al 2002). However, the effect of transgenically expressed enhancin in other plants and to other lepidopteran insects is unknown.…”

mentioning

confidence: 99%

Expression of an enhancin gene from the Trichoplusia ni granulosis virus confers resistance to lepidopterous insect pests to rice

Mori¹,

Kitamura²,

Kondo

et al. 2006

Plant Biotechnology

Self Cite

View full text Add to dashboard Cite

Transgenic plants that produce anti-insect substances are vital in improving crop yields and in reducing the environmental risks of chemical insecticides. Enhancin is a metalloprotease produced in occlusion bodies of the Trichoplusia ni granulovirus (TnGV). It is a key substance that enhances infection of the nucleopolyhedrovirus in lepidopteran insects. Rice (Oryza sativa L. cv. Nipponbare) protoplasts were cotransformed with pREXFVEF and pLTHyg, which respectively bear the chimeric enhancin gene and the hygromycin-resistance gene. Hygromycin-resistant regeneration plants were examined by genomic polymerase chain reaction and genomic Southern and northern blotting analyses to confirm the presence and expression of the enhancin gene. Fourteen transgenic plant lines harboring the enhancin gene were obtained, and stable inheritance and expression of the enhancin gene were confirmed in the second, third, and fourth plant generations. Feeding Spodoptera exigua larvae leaves of enhancin-expressing rice plants in the presence of S. exigua nucleopolyhedrovirus occlusion bodies enhanced infection of the virus. Further, the development of Pseudaletia separata, S. exigua, and S. litura, none of which are host insects of TnGV, was inhibited when these larvae were fed enhancinexpressing rice leaves. This indicates that expression of the enhancin gene confers resistance to lepidopteran insect pests in rice.

show abstract

“…Disruption of the PM increases viral infection and leads to faster larval mortality (Wang et al 1994). Spodoptera exigua larvae fed on artificial diet containing freezedried leaf powder (1.5% w/w) from transgenic tobacco carrying a Tn-En gene showed a tenfold decrease in LD 50 when infected with Autographa californica nucleopolyhedrovirus occlusion bodies, in comparison with insects fed virus supplemented with leaf powder from nontransformed tobacco (Hayakawa et al 2000). These results suggest that enhancin may have potential as a novel insecticide protein, either alone or in combination with Bt proteins or other insect control measures.…”

Section: Introductionmentioning

confidence: 99%

Transgenic tobacco plants carrying a baculovirus enhancin gene slow the development and increase the mortality of Trichoplusia ni larvae

et al. 2002

View full text Add to dashboard Cite

This study was done to assess insect growth and mortality on tobacco plants transformed with baculovirus enhancin genes, as a first step toward the possible use of enhancin transgenes as part of an insect control system. Enhancin genes from Trichoplusia ni or Helicoverpa armigera baculoviruses were introduced into tobacco via Agrobacterium tumefaciens with kanamycin selection. PCR analyses of genomic DNA confirmed the presence of the enhancin genes in the kanamycin-resistant plants; however, the expression of the genes was very low and could be detected only with RT-PCR. Bioassays with Trichoplusia ni larvae showed that larval growth and development was significantly slower on some transgenic lines and that larval mortality was higher. The majority of the enhancin-transgenic plants had little or no inhibitory effect. The low expression of enhancin in plants carrying current expression cassettes and the relevance of these results to pest management are discussed.

show abstract

Enhancement of baculovirus infection in Spodoptera exigua (Lepidoptera: Noctuidae) larvae with Autographa californica nucleopolyhedrovirus or Nicotiana tabacum engineered with a granulovirus enhancin gene.

Cited by 31 publications

References 22 publications

Molecular structure of the peritrophic membrane (PM): Identification of potential PM target sites for insect control

Molecular structure of the peritrophic membrane (PM): Identification of potential PM target sites for insect control

Expression of an enhancin gene from the Trichoplusia ni granulosis virus confers resistance to lepidopterous insect pests to rice

Transgenic tobacco plants carrying a baculovirus enhancin gene slow the development and increase the mortality of Trichoplusia ni larvae

Contact Info

Product

Resources

About