Enhancement of Biocontrol Efficacy of Pichia kudriavzevii Induced by Ca Ascorbate against Botrytis cinerea in Cherry Tomato Fruit and the Possible Mechanisms of Action

Sun, Keyu; Wang, Ziwuzhen; Wei, Ze; Xue, Zhang; Lei, Li; Fu, Yaning; Gao, Jianhua; Zhao, Xin; Guo, Jun; Wang, Junping

doi:10.1128/spectrum.01507-21

Cited by 8 publications

(4 citation statements)

References 53 publications

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“…31 Combined applications of MAPs with antifungal substances or antagonistic microbes have shown better control of rots from the first sampling point at 5 days of CS, although significant reduction appeared only on the final sampling day at 40 days of CS. The antagonistic mechanisms reported in M. pulcherrima, competition for nutrients such as iron depletion, 32,44 parasitism, 32 and induction of host defence response, 45 and in P. kudriavzevii the production of antifungal volatile organic compounds 33 may be involved in the control of common cherry postharvest pathogens, such as Monilinia spp., Cladosporium cladosporioides, B. cinerea, P. expansum, and Penicillium glabrum. The application of these three antifungal treatments was also effective in controlling decay during SL, mainly at the final sampling point, where significant reductions were observed (P < 0.050).…”

Section: Discussionmentioning

confidence: 99%

Improvement of shelf‐life of cherry (Prunus avium L.) by combined application of modified‐atmosphere packaging and antagonistic yeast for long‐distance export

et al. 2023

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BACKGROUND The last decade has seen a growing interest in reducing the use of chemical fungicides for postharvest decay control. In the research for new, safe alternatives, the combined application of biocontrol agents and passive modified‐atmosphere packaging (MAP) has been shown to be a promising strategy to extend fruit quality. Therefore, the aim of this work was to evaluate the effect of the combined application of MAP and two antagonistic yeasts, Metschnikowia pulcherrima L672 and Pichia kudriavzevii PK18, on sweet cherry shelf life. RESULTS Microbiological, physico‐chemical, and quality fruit analysis from batches treated with antagonistic yeast were compared with a control batch without yeast application and a batch to which fludioxonil (Scholar®) was applied. The composition of the atmosphere and physico‐chemical traits showed similar values among the different batches during cold storage. However, interestingly, the combination of MAP with the antagonistic yeasts M. pulcherrima L672 and P. kudriavzevii PK18 increases the control of microbiological spoilage with results comparable to the application of fludioxonil. In addition, these batches experienced a slight decrease in volatile compounds associated with fresh fruit aroma, whereas in the control batch an increase of altered fruit aromas was observed. The same effect of control of spoilage was observed during the shelf life period. CONCLUSION These results showed the positive effect of the combination of antagonistic yeasts and MAP, obtaining similar results in terms of control of microbiological spoilage and physico‐chemical quality compared with the application of fludioxonil. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Improvement of shelf‐life of cherry (Prunus avium L.) by combined application of modified‐atmosphere packaging and antagonistic yeast for long‐distance export

et al. 2023

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“…A notable finding in this research is the identification of three fungi thriving on PKC that exhibit antifungal properties. [43,44]. Further investigation could explore the potential introduction of Candida species into the substrate before storage as a strategy to counter the proliferation of mycotoxin-producing fungi [43,45].…”

Section: Measures Against Fungal Invasion Of Pkcmentioning

confidence: 99%

“…[43,44]. Further investigation could explore the potential introduction of Candida species into the substrate before storage as a strategy to counter the proliferation of mycotoxin-producing fungi [43,45]. Concerning the laws and rules made by authorities to fight the spread of mycotoxins in animal feed, we suggest conducting regular studies that determine the levels of mycotoxins and the presence of mycotoxin-producing fungi in animal food.…”

Section: Measures Against Fungal Invasion Of Pkcmentioning

confidence: 99%

Addressing the Concern of Orange-Yellow Fungus Growth on Palm Kernel Cake: Safeguarding Dairy Cattle Diets for Mycotoxin-Producing Fungi

Bastidas-Caldes,

Vasco-Julio,

Huilca-Ibarra

et al. 2024

Microorganisms

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Palm kernel cake (PKC), a byproduct of palm oil extraction, serves an important role in Ecuador’s animal feed industry. The emergence of yellow-orange fungal growth in PKC on some cattle farms in Ecuador sparked concerns within the cattle industry regarding a potential mycotoxin-producing fungus on this substrate. Due to the limited availability of analytical chemistry techniques in Ecuador for mycotoxin detection, we chose to isolate and identify the fungus to determine its association with mycotoxin-producing genera. Through molecular identification via ITS region sequencing, we identified the yellow-orange fungus as the yeast Candida ethanolica. Furthermore, we isolated two other fungi—the yeast Pichia kudriavzevii, and the fungus Geotrichum candidum. Molecular identification confirmed that all three species are not classified as mycotoxin-producing fungi but in contrast, the literature indicates that all three have demonstrated antifungal activity against Aspergillus and Penicillium species, genera associated with mycotoxin production. This suggests their potential use in biocontrol to counter the colonization of harmful fungi. We discuss preventive measures against the fungal invasion of PKC and emphasize the importance of promptly identifying fungi on this substrate. Rapid recognition of mycotoxin-producing and pathogenic genera holds the promise of mitigating cattle intoxication and the dissemination of mycotoxins throughout the food chain.

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“…In this case, P. kudriavzevii was the dominant species at the initial storage period, but its relative abundance gradually decreased during the whole storage process, while the succession of pathogens was disrupted and the abundance of several other antagonistic yeasts increased, indicating that the biocontrol effectiveness of P. kudriavzevii relies on the altering of the fungal population community, but not on its competitive advantage [ 148 ]. In contrast, in another study highlighting its role in nutrition and space competition, the energy metabolism and nutrient utilization efficiency of P. kudriavzevii were enhanced by the synergistic promotion effect of calcium ascorbate treatment, resulting in improved biocontrol efficacy against cherry tomato gray mold [ 149 ]. Overall, similar to other antagonistic yeasts, multiple mechanisms cooperatively work in the biological control of P. kudriavzevii , and the complex interactions among the host, microbiome and environment remains to be further elucidated.…”

Section: Applications In Biotechnologymentioning

confidence: 99%

Advances in the Application of the Non-Conventional Yeast Pichia kudriavzevii in Food and Biotechnology Industries

Chu

Jin

et al. 2023

JoF

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Pichia kudriavzevii is an emerging non-conventional yeast which has attracted increased attention for its application in food and biotechnology areas. It is widespread in various habitats and often occurs in the spontaneous fermentation process of traditional fermented foods and beverages. The contributions of P. kudriavzevii in degrading organic acid, releasing various hydrolase and flavor compounds, and displaying probiotic properties make it a promising starter culture in the food and feed industry. Moreover, its inherent characteristics, including high tolerance to extreme pH, high temperature, hyperosmotic stress and fermentation inhibitors, allow it the potential to address technical challenges in industrial applications. With the development of advanced genetic engineering tools and system biology techniques, P. kudriavzevii is becoming one of the most promising non-conventional yeasts. This paper systematically reviews the recent progress in the application of P. kudriavzevii to food fermentation, the feed industry, chemical biosynthesis, biocontrol and environmental engineering. In addition, safety issues and current challenges to its use are discussed.

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Enhancement of Biocontrol Efficacy of Pichia kudriavzevii Induced by Ca Ascorbate against Botrytis cinerea in Cherry Tomato Fruit and the Possible Mechanisms of Action

Abstract: Antagonistic yeast is a promising way to control postharvest fruit decay because of its safety and broad-spectrum resistance. However, the biocontrol efficacy of yeast is limited by environmental stress, such as oxidative stress.

Cited by 8 publications

References 53 publications

Improvement of shelf‐life of cherry (Prunus avium L.) by combined application of modified‐atmosphere packaging and antagonistic yeast for long‐distance export

Improvement of shelf‐life of cherry (Prunus avium L.) by combined application of modified‐atmosphere packaging and antagonistic yeast for long‐distance export

Addressing the Concern of Orange-Yellow Fungus Growth on Palm Kernel Cake: Safeguarding Dairy Cattle Diets for Mycotoxin-Producing Fungi

Advances in the Application of the Non-Conventional Yeast Pichia kudriavzevii in Food and Biotechnology Industries

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