Enhancement of carcinoembryonic antibody immobilization on gold electrode modified by gold nanoparticles and SiO2/Thionine nanocomposite

Zhuo, Ying; Yu, Rongjie; Yuan, Ruo; Chai, Yaqin; Hong, Chenglin

doi:10.1016/j.jelechem.2009.01.016

Cited by 54 publications

(22 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nanoparticles have often been used in constructing electrochemical biosensors due to their special features including their large surface area, high surface free energy, and good biocompatibility [12,13]. For example, metal nanoparticles can be used to promote electron transfer between proteins and electrodes.…”

Section: Introductionmentioning

confidence: 99%

“…For example, metal nanoparticles can be used to promote electron transfer between proteins and electrodes. Particularly, Au nanoparticles (nano-Au) have been extensively used as an immobilized matrix to promote the direct electron transfer of the immobilized redox proteins [10][11][12][13][14][15][16]. Xiao et al developed a biosensor based on HRP immobilized on nano-Au.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles

Yuan

Zhuo

et al. 2010

Appl Biochem Biotechnol

Self Cite

View full text Add to dashboard Cite

In this work, an enzyme biosensor based on the immobilization of horseradish peroxidase (HRP) on SiO₂/BSA/Au/thionine/nafion-modified gold electrode was fabricated successfully. Firstly, nafion was dropped on the surface of the gold electrode to form a nafion film followed by chemisorption of thionine (Thi) as an electron mediator via the ion-exchange interaction between the Thi and nafion. Subsequently, the SiO₂/BSA/Au composite nanoparticles were assembled onto Thi film through the covalent bounding with the amino groups of Thi. Finally, HRP was immobilized on the SiO₂/BSA/Au composite nanoparticles due to the covalent conjugation to construct an enzyme biosensor. The surface topographies of the SiO₂/BSA/Au composite nanoparticles were investigated by using scanning electronic microscopy. The stepwise self-assemble procedure of the biosensor was further characterized by means of cyclic voltammetry and chronoamperometry. The enzyme biosensor showed high sensitivity, good stability and selectivity, a wide linear response to hydrogen peroxide (H₂O₂) in the range of 8.0 x 10⁻⁶ ~3.72 x 10⁻³ mol/L, with a detection limit of 2.0 x 10⁻⁶ mol/L. The Michaelies-Menten constant K(app)(M) value was estimated to be 2.3 mM.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles

Yuan

Zhuo

et al. 2010

Appl Biochem Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…SAM can be prepared via two methods: immersion and electrochemical growth [114,149]. The immersion growth is a common method for SAMs' formation through which the metal electrode is soaked in the required solution for a certain time then the electrode was washed with the same solvent and dried [114].…”

Section: Methods Of Sam Preparationmentioning

confidence: 99%

“…In addition, the assemblies formed by immersion growth sometimes were subjected to nonuniform layer growth. The electrochemical method was free of the above drawbacks and more advantageous than immersion growth such as controllable and selective [149].…”

Section: Methods Of Sam Preparationmentioning

confidence: 99%

See 1 more Smart Citation

Self-Assembled Monolayers on Nano-structured Composites for Electrochemical Sensing Applications

Atta¹,

El-Ads²,

Galal³

2015

Handbook of Nanoelectrochemistry

View full text Add to dashboard Cite

Self-assembled monolayer (SAM) represents one of the methods to precisely modify surface structures in the nanoscale dimension. It has opened up a new era of exploration and has a profound impact on sensors and biosensors due to its unique properties. Different self-assemblies will be considered in this chapter: SAM of metallic nanoparticles on polymeric film, SAM of surfactant on polymeric film, and SAM of S-containing compounds on nanometallic films. The main goal of this chapter is to present comprehensive collection of the recent achievements in this area. Several issues will be discussed including the morphology, sensitivity, selectivity, stability, and electrochemical properties of the sensor.

show abstract

Gold Nanoparticle‐Based Electrochemical Biosensors for Medical Applications

Anık

2012

Biomedical Materials and Diagnostic Devices

View full text Add to dashboard Cite

Enhancement of carcinoembryonic antibody immobilization on gold electrode modified by gold nanoparticles and SiO2/Thionine nanocomposite

Cited by 54 publications

References 44 publications

Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles

Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles

Self-Assembled Monolayers on Nano-structured Composites for Electrochemical Sensing Applications

Gold Nanoparticle‐Based Electrochemical Biosensors for Medical Applications

Contact Info

Product

Resources

About