Enhancement of cell adhesion, retention, and survival of HUVEC/cbMSC aggregates that are transplanted in ischemic tissues by concurrent delivery of an antioxidant for therapeutic angiogenesis

Insight on stem cell preconditioning and instructive biomaterials to enhance cell adhesion, retention, and engraftment for tissue repair

“…Moreover, engraftment of transplanted cells with a vascular system may enhance cell viability especially in the case of thick tissue constructs. One strategy for augmenting integration between transplanted cells and host cells may be the use of angiogenic growth factors along with transplanted cells to promote vascularization [122,[287][288][289]. Alternatively, transplantation of cells or progenitors in concert with bioactive molecules such as SDF-1a or neuropeptide substance P may also contribute to the formation of new blood vessels that enhance cell survival and tissue repair [290,291].…”

Therapeutic Potential of Human Mesenchymal Stem Cells for Treating Ischemic Limb Diseases

“…Cell aggregates with human umbilical vein endothelial cells (HUVECs) and umbilical cord blood-derived human MSCs were shown to enhance blood perfusion, capillary density, and arteriole density. These aggregates were also shown to reduce the fibrotic areas in the ischemic limb tissues of mice because of their bulkiness and effective entrapment at the transplantation sites (6). Combination therapies such as microgels with gene modifications or spheroids with low-level light have also been developed to enhance angiogenesis in the context of animal models of limb ischemia (12, 32).…”

In Vitro Anti-AngiogenicActivityand Anti-Cancer Properties of ThiadiazoleCoumarin Derivative Compound

“…Tube formation assay is an in vitro quantitative assay performed to evaluate the anti-angiogenic activity of the chemical compound and to confirm the ability of EAHY to form capillary-like structures on a matrigel matrix, where the cells adhere into the basement membrane, they start to migrate and differentiate to form tubes-network [17].Briefly, the matrigel matrix was allowed to polymerizefor 45 min at 37°C in 5% CO 2 . EAHY were trypsinized and seeded in 100 μl of DMEM containing various concentrations of TCD(4) compound in triplicates.…”