Enhancement of Cell Growth and Lipid Content of a Freshwater Microalga <i>Scenedesmus</i> sp. by Optimizing Nitrogen, Phosphorus and Vitamin Concentrations for Biodiesel Production

Abstract

The effects of nitrogen, phosphorus and vitamin on growth and lipid content of a freshwater microalgae Scenedesmus sp. were investigated under indoor conditions. Nitrogen, phosphorus and vitamin presented significant statistical effect on lipid content, and a maximum lipid content of 29.3% was obtained, corresponded to increases of 90% when compared to those obtained with the original nutrients medium. On the other hand, only nitrogen and phosphorus presented significant statistical effect on cell growth, achieving ash free dry biomass, algal density, and specific growth rate of 1.3 g•L −1 , 1.5E+07 cells mL −1 , and 0.62 d −1 , respectively. By the use of the desirability function, it was possible to maximize the cell growth and lipid content simultaneously. The fatty acid composition of the microalgal lipid comprises over 80% of saturated and monounsaturated fatty acids. Thus, Scenedesmus sp. biomass could be used as suitable feedstock for biodiesel production.

An overview of the utilisation of microalgae biomass derived from nutrient recycling of wet market wastewater and slaughterhouse wastewater

“…Microalgae play a vital role in food production and the purification of contaminated environment water by assimilating nutrients from water and wastewater (Ramaraj et al 2015;Baharuddin et al 2016). They play important roles in the food chain, since they represent the main food for rotifers, cladocerans, fish, and shrimp larvae due to their high nutritional value which includes lipid, protein, carotene, vitamins, carbohydrates, and other essential minerals for the development of aquaculture organisms (Hakalin et al 2014;Baharuddin et al 2016). Among several species of microalgae, Chlorella sp., Scenedesmus sp., and Spirulina sp.…”

Treatment of second cheese whey effluents using aChoricystis-based system with simultaneous lipid production

“…Usually, the presence of unsaturated fatty acids leads to a lowered biodiesel cetane number and increased NOx emission. Unsaturated fatty acids are prone to oxidation, which would also affect the lubricity of a biodiesel . Hence, it is essential that the biodiesel has high levels of saturated and monounsaturated fatty acids with low levels of polyunsaturated fatty acids.…”

Growth Evaluation of Microalgae Isolated From Palm Oil Mill Effluent in Synthetic Media

“…The lipid standard curve was established by using cholesterol. The values of lipid content (%) and productivity were calculated using the equations below [11].…”

“…The cell weight was measured using an analytical balance (XB 220A, Switzerland) and calculated using Equation (4). Besides, the biomass productivity of microalgae was calculated using Equation (5) below [11].…”

“…In fact, approximately 50% of carbon is present in microalgae biomass and this carbon is obtained from the utilisation of CO 2 [8]. Furthermore, important biochemical, such as polyunsaturated fatty acids, pigments, lipids, proteins, carbohydrates, and vitamins, can be extracted from algae to be used in the production of high-value products such as fertilisers, cosmetics, and drugs [11][12][13][14][15]. Moreover, several different systems are available to grow algae in industrial sectors, namely open ponds, covered ponds, raceways, and engineered systems (photobioreactors) [13,16].…”