Enhancement of Culture of Legionella longbeachae from Respiratory Samples by Use of Immunomagnetic Separation and Antimicrobial Decontamination

Mohammadi, Ali; Chambers, Stephen; Scott-Thomas, Amy; Lewis, John G.; Anderson, Trevor P.; Podmore, Ros; Williman, Jonathan; Murdoch, David R.; Slow, Sandy

doi:10.1128/jcm.01218-20

Cited by 4 publications

(1 citation statement)

References 32 publications

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“… 4 , 5 Although there are some bacterial species that cannot be identified by mass spectrometry, L. longbeachae is considered identifiable using this method. 6 We obtained culture medium, humus, and field soil from the patient’s home. The samples were acid-treated using 0.2 M HCl-KCl buffer (pH 2.2) and incubated with B-CYE alfa for 1 week at 37°C.…”

Section: Case Presentationmentioning

confidence: 99%

A case of severe pneumonia caused by Legionella longbeachae with positive results by a Legionella urinary antigen detection kit

Shinomiya

Tanaka

Shionoya

et al. 2023

Therapeutic Advances in Infection

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Legionella longbeachae is a Legionella bacteria often detected in soil, and is known as a rare cause of Legionella infections in Japan. In addition, detection of this Legionella species is often overlooked due to negative results from Legionella urinary antigen tests, which could lead to errors in the therapeutic approach. An 80-year-old woman was admitted to our hospital because of fever and dyspnea. Her blood tests showed elevated white blood cells, increased C-reactive protein and transaminases, and hyponatremia. Chest computed tomography showed dense consolidation in the right lung. We diagnosed Legionella pneumonia because the Legionella urinary antigen test was positive on the day after her admission. The patient was intubated and mechanically ventilated on the third day of hospitalization, because of respiratory failure. However, her condition did not improve and she died on the 10th day after admission. After her death, L. longbeachae was detected from sputum culture from her tracheal tube, and was diagnosed as the causative organism of her pneumonia. L. longbeachae infection reportedly rarely produces positive urinary antigen test results. Our experience suggests that the urinary antigen test using Ribotest Legionella might be able to detect Legionella spp. other than L. pneumophila.

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Section: Case Presentationmentioning

confidence: 99%

A case of severe pneumonia caused by Legionella longbeachae with positive results by a Legionella urinary antigen detection kit

Shinomiya

Tanaka

Shionoya

et al. 2023

Therapeutic Advances in Infection

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The inhibitory effect of copper, zinc, and manganese on Legionella longbeachae and other Legionella spp. in vitro

Jun

Scott-Thomas

Slow

et al. 2023

Letters in Applied Microbiology

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Legionella longbeachae is an important cause of Legionnaires’ disease in Australasia and is associated with exposure to potting soils. Our aim was to identify ways to reduce the load of L. longbeachae in potting soils. Inductively-coupled plasma optical emission spectrometry (ICP-OES) of an all-purpose potting mix showed copper (Cu) concentrations (mg/kg) range from 15.8–23.6. Zinc (Zn) and manganese (Mn) were significantly higher than Cu ranging from 88.6–106 and 171–203 respectively. Minimal inhibitory and bactericidal concentrations of 10 salts used in the horticultural industry were determined for Legionella species in buffered yeast extract (BYE) broth. For L. longbeachae (n = 9) the median (range) minimum inhibitory concentration (MIC) (mg/L) of copper sulfate was 31.25 (15.6–31.25), zinc sulfate 31.25 (7.81–31.25) and manganese sulfate 31.25 (7.81–62.5). The MIC and minimum bactericidal concentration (MBC) were within one dilution of each other. Susceptibility to Cu and Zn salts increased as the concentration of pyrophosphate iron in the media decreased. The MIC values for these three metals against L. pneumophila (n = 3) and L. micdadei (n = 4) were similar. Combinations of Cu, Zn and Mn were additive. L. longbeachae has similar susceptibility to Cu and other metal ions in comparison to L. pneumophila.

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Clinical and Laboratory Diagnosis of Legionella Pneumonia

Bai

Yang

2023

Diagnostics

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Legionella pneumonia is a relatively rare but extremely progressive pulmonary infection with high mortality. Traditional cultural isolation remains the gold standard for the diagnosis of Legionella pneumonia. However, its harsh culture conditions, long turnaround time, and suboptimal sensitivity do not meet the clinical need for rapid and accurate diagnosis, especially for critically ill patients. So far, pathogenic detection techniques including serological assays, urinary antigen tests, and mass spectrometry, as well as nucleic acid amplification technique, have been developed, and each has its own advantages and limitations. This review summarizes the clinical characteristics and imaging findings of Legionella pneumonia, then discusses the advances, advantages, and limitations of the various pathogenetic detection techniques used for Legionella pneumonia diagnosis. The aim is to provide rapid and accurate guiding options for early identification and diagnosis of Legionella pneumonia in clinical practice, further easing healthcare burden.

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Enhancement of Culture of Legionella longbeachae from Respiratory Samples by Use of Immunomagnetic Separation and Antimicrobial Decontamination

Cited by 4 publications

References 32 publications

A case of severe pneumonia caused by Legionella longbeachae with positive results by a Legionella urinary antigen detection kit

A case of severe pneumonia caused by Legionella longbeachae with positive results by a Legionella urinary antigen detection kit

The inhibitory effect of copper, zinc, and manganese on Legionella longbeachae and other Legionella spp. in vitro

Clinical and Laboratory Diagnosis of Legionella Pneumonia

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