Enhancement of Dichomitus squalens tolerance to copper and copper-associated laccase activity by carbon and nitrogen sources

Kannaiyan, Ranjani; Mahinpey, Nader; Mani, Thilakavathi; Martinuzzi, Robert J.; Kostenko, Victoria

doi:10.1016/j.bej.2012.06.007

Cited by 15 publications

(10 citation statements)

References 23 publications

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“…No impact on fungi growth was observed; therefore, high Mn 2+ levels have imposed toxicity only towards peroxidase production and/or activity. A similar effect was observed for the response of D. squalens laccase to copper sulphate . Authors also reported that both inducing and suppressing effects of copper on laccase activity were regulated by the type and proportion of carbon and nitrogen sources in the media.…”

Section: Discussionsupporting

confidence: 67%

Induction and suppression of Dichomitus squalens and Ceriporiopsis subvermispora peroxidase activity by manganese sulphate in response to carbon and nitrogen sources

et al. 2013

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Delignification is critical in the production of biofuel from a potentially abundant renewable resource, lignocellulosic waste. One of the classes of lignolytic enzymes are peroxidases. Manganese sulphate (Mn2+) has a significant impact on the peroxidase activity of lignolytic fungi. Along with induction, the suppression of peroxidase activity by Mn2+ has been observed. Peroxidase regulation is governed by three critical Mn2+ concentrations: the minimum inductive concentration (MIC), the peak concentration (PC) and the minimum suppressive concentration (MSC). The induction and suppression of enzyme activity were not associated with fungal growth capacity, but with a specific enzyme response to the nutritional conditions. Manipulation of the carbon and nitrogen sources shifted the peroxidase suppression by Mn2+ to high concentrations and, hence, increased the peroxidase tolerance to Mn2+ and, consequently, peak peroxidase activities. The manipulation of carbon and nitrogen sources allowed increasing the peak concentrations of Mn2+ and corresponding peroxidase activity up to 0.5 mg/mL and 180.70 AU (173% increase compared to standard) in C. subvermispora, and up to 0.05 mg/mL and 151.23 AU in D. squalens (91% increase compared to standard). Thus, manipulation of carbon and nitrogen sources is a useful tool in enhancing fungi peroxidase tolerance to Mn2+ and improving the delignification of lignocellulosic biomass by fungi to prepare an easily consumable substrate for economically viable biofuel production.

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Section: Discussionsupporting

confidence: 67%

Induction and suppression of Dichomitus squalens and Ceriporiopsis subvermispora peroxidase activity by manganese sulphate in response to carbon and nitrogen sources

et al. 2013

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“…Copper present in higher concentrations of its free, cupric form is extremely toxic to fungal cells (Galhaup and Haltrich 2001 ). The mechanism of the Lcc induction by copper is associated with its role in the Lcc active centre, and its participation in the regulation of Lcc genes transcription and post-transcription modifications; whereas, copper toxicity is attributed to the interaction of copper ions with proteins, enzymes, nucleic acids, and metabolites associated with cell functions and viability, and due to oxidative (Kannaiyan et al 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…According to Kannaiyan et al ( 2012 ), the induction of Dichomitus squalens Lcc activity required copper sulphate addition as low as 0.06 mM. Palanisami and Lakshmanan ( 2011 ) using the marine filamentous non-heterocystous cyanobacterium Phormidium valderianum observed a negative effect of Lcc activity when the concentration of copper was greater than 10 μM and, consequently, a gradual decrease in the rate of Poly-R478 dye decolourisation.…”

Section: Discussionmentioning

confidence: 99%

Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330

et al. 2015

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The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1–10 μM) was assessed. In a liquid culture medium supplemented with 5 μM of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U·L−1). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

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“…Lignin production is carried out by phenylalanine ammonia-lyase (PAL) and cinnamyl alcohol-NADPH-dehydrogenase (CAD), which are regulated by extracellular Cu-dependent laccases and peroxidases [51,52]. Kannaiyan et al (2012) found that there is a peak Cu concentration at which laccase activity switches from being promoted to repressed and/or terminated [53]. This threshold concentration reflects the Cu tolerance of the organism; at lower concentrations Cu acts as a cofactor and at higher concentrations Cu toxicity is observed.…”

Section: Stamp Sandmentioning

confidence: 99%

Evaluation of Copper-Contaminated Marginal Land for the Cultivation of Vetiver Grass (Chrysopogon zizanioides) as a Lignocellulosic Feedstock and its Impact on Downstream Bioethanol Production

2019

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Metal-contaminated soil could be sustainably used for biofuel feedstock production if the harvested biomass is amenable to bioethanol production. A 60-day greenhouse experiment was performed to evaluate (1) the potential of vetiver grass to phytostabilize soil contaminated with copper (Cu), and (2) the impact of Cu exposure on its lignocellulosic composition and downstream bioethanol production. Dilute acid pretreatment, enzymatic hydrolysis, and fermentation parameters were optimized sequentially for vetiver grass using response surface methodology (RSM). Results indicate that the lignocellulosic composition of vetiver grown on Cu-rich soil was favorably altered with a significant decrease in lignin and increase in hemicellulose and cellulose content. Hydrolysates produced from Cu exposed biomass achieved a significantly greater ethanol yield and volumetric productivity compared to those of the control biomass. Upon pretreatment, the hemicellulosic hydrolysate showed an increase in total sugars per liter by 204.7% of the predicted yield. After fermentation, 110% of the predicted ethanol yield was obtained for the vetiver grown on Cu-contaminated soil. By contrast, for vetiver grown on uncontaminated soil a 62.3% of theoretical ethanol yield was achieved, indicating that vetiver has the potential to serve the dual purpose of phytoremediation and biofuel feedstock generation on contaminated sites.

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Enhancement of Dichomitus squalens tolerance to copper and copper-associated laccase activity by carbon and nitrogen sources

Cited by 15 publications

References 23 publications

Induction and suppression of Dichomitus squalens and Ceriporiopsis subvermispora peroxidase activity by manganese sulphate in response to carbon and nitrogen sources

Induction and suppression of Dichomitus squalens and Ceriporiopsis subvermispora peroxidase activity by manganese sulphate in response to carbon and nitrogen sources

Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330

Evaluation of Copper-Contaminated Marginal Land for the Cultivation of Vetiver Grass (Chrysopogon zizanioides) as a Lignocellulosic Feedstock and its Impact on Downstream Bioethanol Production

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