Enhancement of DNA vaccine potency by sandwiching antigen-coding gene between secondary lymphoid tissue chemokine (SLC) and IgG Fc fragment genes

Liu, Rongzhi; Zhou, Chunxia; Wang, Dongmei; Ma, Wenbo; Chen, Lin; Wang, Yongquan; Liang, Xiao; Li, Jie; Guo, Sujuan; Wang, Yihua; Zhang, Youhui; Zhang, Shuren

doi:10.4161/cbt.5.4.2528

Cited by 17 publications

(9 citation statements)

References 24 publications

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“…The connection between myocardial production of IFN- γ and cardiomyocyte gene expression changes leading to hypertrophy, ventricular dilation, and heart failure was previously unknown and unexpected and was only identified because of the use of a data-driven “omics”/systems biology approach. It is known that other inflammatory mediators such as TNF- α , CCL2 [38], IL-18, CCL21, and phosphorylated Smad2 involved in TGF β signaling [43], upregulated in CCC myocardium, are able to induce cardiomyocyte hypertrophy and fibrosis [44, 45]. Taken together, data suggest that locally produced inflammatory mediators have nonimmunological effects on myocardial tissue distinct from direct tissue damage, which may play a significant pathogenic role in CCC, by modulating gene and protein expression in pathways essential to the development of CCC.…”

Section: Immunological Dynamics During the Acute And Chronic Phasementioning

confidence: 99%

Chagas Disease Cardiomyopathy: Immunopathology and Genetics

Cunha-Neto

Chevillard

2014

Mediators of Inflammation

185

165

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Chagas disease, caused by the protozoan Trypanosoma cruzi, is endemic in Latin America and affects ca. 10 million people worldwide. About 30% of Chagas disease patients develop chronic Chagas disease cardiomyopathy (CCC), a particularly lethal inflammatory cardiomyopathy that occurs decades after the initial infection, while most patients remain asymptomatic. Mortality rate is higher than that of noninflammatory cardiomyopathy. CCC heart lesions present a Th1 T-cell-rich myocarditis, with cardiomyocyte hypertrophy and prominent fibrosis. Data suggest that the myocarditis plays a major pathogenetic role in disease progression. Major unmet goals include the thorough understanding of disease pathogenesis and therapeutic targets and identification of prognostic genetic factors. Chagas disease thus remains a neglected disease, with no vaccines or antiparasitic drugs proven efficient in chronically infected adults, when most patients are diagnosed. Both familial aggregation of CCC cases and the fact that only 30% of infected patients develop CCC suggest there might be a genetic component to disease susceptibility. Moreover, previous case-control studies have identified some genes associated to human susceptibility to CCC. In this paper, we will review the immunopathogenesis and genetics of Chagas disease, highlighting studies that shed light on the differential progression of Chagas disease patients to CCC.

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Section: Immunological Dynamics During the Acute And Chronic Phasementioning

confidence: 99%

Chagas Disease Cardiomyopathy: Immunopathology and Genetics

Cunha-Neto

Chevillard

2014

Mediators of Inflammation

185

165

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“…There are several reported strategies for using chemokines in cancer immunotherapy ( 102 – 104 ). Among them, transfection of tumor cells in vitro and inoculation in vivo and intratumoral injection of chemokine-encoding vectors are used most frequently.…”

Section: Immune Cell Recruitment and Cell-based System For Cancer Immmentioning

confidence: 99%

Immune Cell Recruitment and Cell-Based System for Cancer Therapy

2007

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Abstract. Immune cells, such as cytotoxic T lymphocytes, natural killer cells, B cells, and dendritic cells, have a central role in cancer immunotherapy. Conventional studies of cancer immunotherapy have focused mainly on the search for an efficient means to prime/activate tumor-associated antigen-specific immunity. A systematic understanding of the molecular basis of the trafficking and biodistribution of immune cells, however, is important for the development of more efficacious cancer immunotherapies. It is well established that the basis and premise of immunotherapy is the accumulation of effective immune cells in tumor tissues. Therefore, it is crucial to control the distribution of immune cells to optimize cancer immunotherapy. Recent characterization of various chemokines and chemokine receptors in the immune system has increased our knowledge of the regulatory mechanisms of the immune response and tolerance based on immune cell localization. Here, we review the immune cell recruitment and cell-based systems that can potentially control the systemic pharmacokinetics of immune cells and, in particular, focus on cell migrating molecules, i.e., chemokines, and their receptors, and their use in cancer immunotherapy.

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“…Our previous results have illustrated that SLC and Fc can synergistically enhance the immunogenicity of an E7-expressing plasmid DNA vaccine, resulting in dramatic augmentation of systemic anti-tumour immunity against E7-expressing tumours. 15 …”

Section: Introductionmentioning

confidence: 99%

Bacterial magnetic particles as a novel and efficient gene vaccine delivery system

Zhou

et al. 2011

Gene Ther

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DNA vaccination is an attractive approach for eliciting antigen-specific immunity. In this study, we used magnetosomes (bacterial magnetic particles, BMPs) as carriers of a recombinant DNA composed of a secondary lymphoid tissue chemokine, human papillomavirus type E7 (HPV-E7) and Ig-Fc fragment (pSLC-E7-Fc) to generate a gene vaccine (BMP-V) for tumour immunotherapy. The results indicate that BMPs linked to DNA more efficiently in phosphate-buffered saline (pH=4–5) than in physiological saline. Efficient transfection of BMP-V in vitro and in vivo was achieved when a 600-mT static magnetic field was applied for 10 min. In a mouse tumour model, subcutaneous injection of BMP-V (5 μg, × 3 at 4-day intervals) plus magnetic exposure elicited systemic HPV-E7-specific immunity leading to significant tumour inhibition. The treated mice tolerated BMP-V immunisation well with no toxic side effects, as shown by histopathological examinations of major internal organs. Taken together, these results suggest that BMP can be used as a gene carrier to elicit a systemic immune response.

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Enhancement of DNA vaccine potency by sandwiching antigen-coding gene between secondary lymphoid tissue chemokine (SLC) and IgG Fc fragment genes

Cited by 17 publications

References 24 publications

Chagas Disease Cardiomyopathy: Immunopathology and Genetics

Chagas Disease Cardiomyopathy: Immunopathology and Genetics

Immune Cell Recruitment and Cell-Based System for Cancer Therapy

Bacterial magnetic particles as a novel and efficient gene vaccine delivery system

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