Enhancement of enzymatic degradation of cellulose by application of mixed enzyme systems of different fungal origin

Abstract

Individual culture supernatants of six cellulolytic fungi and their mixed enzyme systems were compared in hydrolysis of pure celluloses and cellulose‐rich wastes. It has been observed that the hydrolysis rate of pure cellulosie substances in general increased by mixed enzymes as compared to the enzymes supernatant derived from only one microbial source. This effect was particularly high when Trichoderma and Penicillium enzyme systems were operated together.

Effect of a Cellulase Treatment on Extraction of Antioxidant Phenols from Black Currant (Ribes nigrum L.) Pomace

“…These results are consistent with previous reports that the cellulase system in fungi includes three major hydrolytic enzymes: (a) an endocellulase that cleaves β-linkages at random; (b) a cellobiohydrolase, which releases cellobiose from either the reducing or the nonreducing end of cellulose; and (c) a β-glucosidase that releases glucose from cellobiose and short-chain cellooligosaccharides , . The low level of β-glucosidase activity was consistent with previous reports that Trichoderma reesei cellulases exert low β-glucosidase activity , ). It has also been reported that Trichoderma cellulases additionally contain at least three endoxylanases, and these enzymes might contribute to the xylanase activity in the C013L preparation.…”

Quantitative Measurement of Fiber Fractions of Cool- and Warm-Season Grass Herbage Using Cell-Wall-Degrading Enzymes

“…A synergistic influence of the various enzymes in microbial cellulase complexes has been demonstrated (Szaká cs- Dobozi et al, 1985). Other researchers (McQueen and Van Soest, 1975;Bughrara et al, 1992) have reported an increase in the amount of neutral detergent residue solubilized by a mixture of cellulase and hemicellulase, compared to the solubilization achieved with the cellulase preparation alone.…”