Enhancement of eritadenine production using three carbon sources, immobilization and surfactants in submerged culture with shiitake mushroom (Lentinula edodes) (Berk.) Singer)

Abstract

In this study, the effect of three carbon sources (mannitol, minced potato and sucrose), two immobilization substrates (alginate and wood cylinders), and three surfactants (Tween 20, Tween 40 and Tween 80), were evaluated on eritadenine production using shiitake (Lentinula edodes) mycelium under submerged cultivation, in shake flasks within 20 days. Eritadenine and biomass were measured by HPLC and gravimetrically, respectively. Alginate immobilization of mycelium promoted significant enhancement of eritadenine yields of 88 mg/L, compared to the control (8.7 mg/L) and wood immobilization (14.8 mg/L). Likewise, eritadenine yields (72.4 mg/L) were enhanced by adding surfactant tween 20 to the broths in 0.5%, than control (8.7 mg/L) without surfactant. Tween 40 and 80 did not improve eritadenine yields, but both produced better biomass values (superior to 5 g/L) than the control (3.9 g/L). All carbon sources (sucrose, mannitol, mince potato, and glucose as control) produced similar low eritadenine yields, with best results (10.2 mg/L) by sucrose, although glucose produced the best biomass yields of 3.9 g/L. Also, carbon sources and the best biomass values did not show significant effect on eritadenine production. pH values in the best eritadenine yielding fermentations went down from 6 to 3-4, but pH had a low correlation with eritadenine yields. Finally, all data obtained in the present study are useful for optimizing culture conditions, towards industrialization of this important health improver metabolite (eritadenine).

Enhancing Eritadenine Production in Submerged Cultures of Shiitake ( Lentinula edodes Berk. Pegler) Using Blue LED Light and Activated Charcoal. Revealing Eritadenine’s Novel In Vitro Bioherbicidal Activity Against Chrysanthemum morifolium

“…Blue LED light induces a substantial enhancement in eritadenine yield, surpassing that of darkness and green light by a factor of 13–14, yielding 165.7 mg/L, compared to 11.2 mg/L in darkness and 12.1 mg/L in the presence of green light, as illustrated in Figure 1 . This concentration represents nearly double the highest results reported in our previous study, which employed mycelial immobilization in calcium alginate [ 8 ] that achieved a yield of 88.1 mg/L. Notably, blue LED light has also been observed to promote higher yields of secondary metabolites in Monascus .…”

“…The quantification method employed in this study followed the procedures outlined in our previous investigation [ 8 ]. An Agillent Technologies 1200 Series apparatus, equipped with a C18 column, was used for this purpose.…”

“…Eritadenine production through submerged cultivation of shiitake mycelium has been performed by various authors, including us [ 5–7 ]. Eritadenine yields have varied from 10.2 mg/L [ 5 ], 25 mg/L [ 6 ] and 88 mg/L [ 8 ]. Nevertheless, these yields continue to be unsatisfactory for further exploration of potential applications or for scaling up production to an industrial level.…”