Enhancement of fibrinolytic enzyme production from Bacillus subtilis via immobilization process onto radiation synthesized starch/dimethylaminoethyl methacrylate hydrogel

Raafat, Amany I.; Araby, Eman; Lotfy, Salah

doi:10.1016/j.carbpol.2011.09.029

Cited by 32 publications

(19 citation statements)

References 26 publications

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“…Entrapped enzyme was more stable in the presence of denaturants like urea due to internal carbohydrate moieties, while surface-immobilized enzyme had superior activity (Matto and Husain 2009). Radiation grafting of substances like acrylamide and dimethylaminoethyl methacrylate onto starch are among the widely used industrial techniques for a high product yield (Dung et al 1995; Raafat et al 2011). …”

Section: Materials Used For Fabrication Of Immobilization Supportsmentioning

confidence: 99%

Enzyme immobilization: an overview on techniques and support materials

2012

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The current demands of the world’s biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. These requirements are inevitable to facilitate large-scale and economic formulation. Enzyme immobilization provides an excellent base for increasing availability of enzyme to the substrate with greater turnover over a considerable period of time. Several natural and synthetic supports have been assessed for their efficiency for enzyme immobilization. Nowadays, immobilized enzymes are preferred over their free counterpart due to their prolonged availability that curtails redundant downstream and purification processes. Future investigations should endeavor at adopting logistic and sensible entrapment techniques along with innovatively modified supports to improve the state of enzyme immobilization and provide new perspectives to the industrial sector.

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Section: Materials Used For Fabrication Of Immobilization Supportsmentioning

confidence: 99%

Enzyme immobilization: an overview on techniques and support materials

2012

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“…Injectable hydrogels as drug delivery and implant materials have attracted increasing attention in the past decades due to their unique advantage of nonsurgical treatment to the patients . Environment sensitive hydrogels, also called “intelligent” or “smart” hydrogels can undergo reversible volume phase transitions or gel–sol phase transitions in response to the external stimuli such as pH, temperature, and glucose concentration, have been used extensively in diverse applications, such as in making artificial muscles, chemicalvalves, immobilization of enzymes and cells, and concentrating dilute solutions in bioseparation, especially in the development of smart drug delivery systems . Design of injectable gels using responsive polymers is, thus, becoming an interesting task because the resultant hydrogels can combine the performances of injectability and controlled release of drugs.…”

Section: Introductionmentioning

confidence: 99%

pH and glucose dually responsive injectable hydrogel prepared by in situ crosslinking of phenylboronic modified chitosan and oxidized dextran

Zhang

et al. 2015

J. Polym. Sci. Part A: Polym. Chem.

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Injectable hydrogels with pH and glucose triggered drug release capability were synthesized based on biocompatible phenylboronic modified chitosan and oxidized dextran through the formation of covalent imine bond and phenylboronate ester. Rheological characterization demonstrated that the gelation rate was rapid, and the moduli of the hydrogels were able to be tuned with chemical composition as well as pH and glucose concentration of the polymer solution. Anticancer drugs could be incorporated inside the hydrogel through the in situ gel forming process and undergo a controlled release by altering pH or glucose concentration. The hydrogels had good biocompatibility with viable and proliferated cells cultured in the three dimensional matrix, and the cell proliferation was suppressed when a small amount of DOX was added, which is benefit for the application of the hydrogels as smart anticancer drug delivery system. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2015, 53, 1235–1244

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“…Some overlapped peaks appearing in these spectraNIP, cholesterol templates and MIP have the characteristic peaks corresponding to OH bands at 3417 cm -1 , C-O-C band at 1164 cm -1 and carbonyl band at 1720 cm −1 . FTIR spectrum of pure cholesterol includes H bonded alcohol at 3417 cm −1 and strong hydrocarbon peaks around 2950 cm −1 and 1466 cm -1 [25]meanwhile the characteristic band corresponding to C=O at 1720 cm -1 disappeared in cholesterol FTIR spectra. HEMA was interacted with cholesterol through H-bonds.…”

Section: Fourier Transfer Infrared (Ftir Analysis)mentioning

confidence: 97%

Radiation Synthesis and Characterization of Cholesterol Molecularly Imprinted Polymer of Crosslinked Hydroxyethyl Methacrylate

2016

IJSR

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Cholesterol-imprinted poly hydroxyethyl methacrylate (MIP) andnon-imprinted polyhydroxyethyl methacrylate (NIP) have been prepared via polymerization by gamma radiation. The functional structure of the imprinted and non-imprinted polymers were characterized by Fourier Transform Infrared spectroscopy (FT-IR); the spectrum showed that the HEMA interacts with cholesterol through H-bonds. The surface morphology of NIP and MIP were investigated by scanning electron microscope (SEM) and the MIP image appeared as a rough surface, while NIP had no pores. The specificity of MIP to interact with the print molecular was studied cholesterol and the results showed a higher cholesterol adsorption by MIP than that by NIP. This work approachesto design robust MIP to mimic natural recognition entities as adsorbed materials of cholesterol.

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Enhancement of fibrinolytic enzyme production from Bacillus subtilis via immobilization process onto radiation synthesized starch/dimethylaminoethyl methacrylate hydrogel

Cited by 32 publications

References 26 publications

Enzyme immobilization: an overview on techniques and support materials

Enzyme immobilization: an overview on techniques and support materials

pH and glucose dually responsive injectable hydrogel prepared by in situ crosslinking of phenylboronic modified chitosan and oxidized dextran

Radiation Synthesis and Characterization of Cholesterol Molecularly Imprinted Polymer of Crosslinked Hydroxyethyl Methacrylate

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