Enhancement of fibroblast growing on the mannosylated surface of cellulose membranes

Birkheur, Simone; Faria-Tischer, Paula Cristina de Sousa; Tischer, César Augusto; Pimentel, Elisângela F.; Endringer, Denise Coutinho; Butera, Anna Paola; Ribeiro-Viana, Renato

doi:10.1016/j.msec.2017.04.006

Cited by 13 publications

(9 citation statements)

References 32 publications

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“…Similar results were also observed (33), which evaluated chemically modified BC membranes and demonstrated good in vitro compatibility with fibroblasts, once the membrane helped in the healing process. Figure 7 -Evaluation of the fibroblast migration capacity of the BC-based hydrogel (Nexfill ® Hydrogel).…”

Section: In Vitro Studiessupporting

confidence: 84%

Bacterial cellulose-based hydrogel for wound healing: characterization and in vitro evaluation

Carbinatto

Sábio²,

Meneguin³

et al. 2018

IJAMB

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Bacterial cellulose (BC) has been considered a promising biopolymer with applications in several areas of knowledge, including medicine, mainly due to its ability to assist in the treatment of dermal lesions. Many groups and companies have been making efforts to develop new BC-based materials in order to add new characteristics and therapeutic possibilities. Recently, Seven Indústria de Produtos Biotecnológicos Ltda company developed a BC-based hydrogel aiming to verify the interaction among the formulation components, its potential for wound healing and biocompatibility studies. BC-based hydrogel was characterized and compared with pristine BC film. Physicochemical characterization includes rheological measurements, thermal analyses, field emission gun - scanning electron microscopy (FE-SEM) and in vitro cell migration. BC-based hydrogel showed adequate interaction among the components of the formulation, which may positively influence its stability. In addition, the BC-based hydrogel accelerated the healing processes demonstrating its potential in dermal lesion treatment.

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Section: In Vitro Studiessupporting

confidence: 84%

Bacterial cellulose-based hydrogel for wound healing: characterization and in vitro evaluation

Carbinatto

Sábio²,

Meneguin³

et al. 2018

IJAMB

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“…In addition, considering biomolecules, as proteins, they have high molecular mass and, in this case, only one thiol group per structure, which would also make it difficult to reach the reactive center on the surface of the film. Other experiences of the group with protein and small molecules conjugation to the surface of cellulosic films via formation of amide bond have been successfully reported 29,30 . Considering that the intermediaries in question are less reactive and more stable, they manage to be in the reactive form until the nucleophile attack occurs to them.…”

Section: Resultsmentioning

confidence: 99%

Diphenyltetrazole Modified Bacterial Cellulose Film: Considerations on Heterogeneous Modification and Bioconjugation

et al. 2020

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Understanding chemical reactions in bacterial cellulose (BC) films is important to control the insertion of biomolecules that can improve their biomedical properties. Diphenyltetrazole molecules are able to react with tiols under UV irradiation and has been useful for immobilize proteins on different materials. In this work, we show the functionalization of a BC film with diphenyltetrazole and subsequent attempts of conjugation of the bovine serum albumin (BSA) to it. Diphenyltetrazole was synthesized in two steps, and functionalization of it with BC was carried out under ultrasound irradiation. Spectroscopic analysis of the film by UV-Vis and FTIR confirmed the success of the reaction. Attempts to conjugate BSA to the modified cellulose film by photoclick reaction failed under these conditions. Although this methodology is important in the conjugation of biomolecules, we understand that its application in cellulosic materials in heterogeneous phase is disadvantaged due to the half-life of the unstable intermediate and the biomolecule's access to it.

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“…These modifications increased both hydrophobic and electrostatic interactions with fibroblast cells, beneficial to promote cell growth for wound dressing applications [ 77 ]. The growth of fibroblast cells was enhanced on the mannosylated surface of bacterial cellulose membranes, achieved by grafting aryl monosaccharides into succinylated bacterial cellulose [ 76 ]. The covalent attachment of carbohydrates onto the surface was made viable through the succinic crosslink and was reported to be beneficial to the stimulation of fibroblast growth, as it is a monosaccharide motif used in cellular recognition.…”

Section: Methods Of Scaffold Modificationmentioning

confidence: 99%

Recent Advances in Modified Cellulose for Tissue Culture Applications

2018

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Tissue engineering is a rapidly advancing field in regenerative medicine, with much research directed towards the production of new biomaterial scaffolds with tailored properties to generate functional tissue for specific applications. Recently, principles of sustainability, eco-efficiency and green chemistry have begun to guide the development of a new generation of materials, such as cellulose, as an alternative to conventional polymers based on conversion of fossil carbon (e.g., oil) and finding technologies to reduce the use of animal and human derived biomolecules (e.g., foetal bovine serum). Much of this focus on cellulose is due to it possessing the necessary properties for tissue engineering scaffolds, including biocompatibility, and the relative ease with which its characteristics can be tuned through chemical modification to adjust mechanical properties and to introduce various surface modifications. In addition, the sustainability of producing and manufacturing materials from cellulose, as well as its modest cost, makes cellulose an economically viable feedstock. This review focusses specifically on the use of modified cellulose materials for tissue culturing applications. We will investigate recent techniques used to promote scaffold function through physical, biochemical and chemical scaffold modifications, and describe how these have been utilised to reduce reliance on the addition of matrix ligands such as foetal bovine serum.

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Enhancement of fibroblast growing on the mannosylated surface of cellulose membranes

Cited by 13 publications

References 32 publications

Bacterial cellulose-based hydrogel for wound healing: characterization and in vitro evaluation

Bacterial cellulose-based hydrogel for wound healing: characterization and in vitro evaluation

Diphenyltetrazole Modified Bacterial Cellulose Film: Considerations on Heterogeneous Modification and Bioconjugation

Recent Advances in Modified Cellulose for Tissue Culture Applications

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