Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments

Xie, Liuming; Wang, Gang; Xie, Jianhua; Chen, XianXiang; Xie, Jiayan; Shi, Xiaoyi; Huang, Zhibing

doi:10.1016/j.crfs.2022.11.011

Cited by 5 publications

(4 citation statements)

References 49 publications

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“…Furthermore, it is different from the results of Xie's study [22] and may be due to the different fermentation strains. Genistein can promote EPS production by regulating membrane permeability, enhancing cell respiration metabolism and monosaccharide precursor synthetase activity, which provides direction for exploring the mechanism of quercetin to increase exopolysaccharide yield [23]. The addition of kaempferol and daidzein reduced the EPS yield to 1.25 g/L and 1.15 g/L, respectively, which decreased by 17.8% and 24.3%.…”

Section: Effects Of Flavonoids On Crude Eps Yieldmentioning

confidence: 97%

Contribution of Quercetin to the Composition and Antioxidant Properties of Monascus Exopolysaccharides

Yang

Meng

Xie

et al. 2023

Foods

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Exopolysaccharides are important metabolites of Monascus with healthy activities. However, the low production level limits their applications. Hence, the aim of this work was to increase the yield of exopolysaccharides (EPS) and optimize liquid fermentation by adding flavonoids. The EPS yield was optimized via both medium composition and culture conditions. The optional fermentation conditions achieved for EPS production of 7.018 g/L were 50 g/L sucrose, 3.5 g/L yeast extract, 1.0 g/L MgSO4·7H2O, 0.9 g/L KH2PO4, 1.8 g/L K2HPO4·3H2O, 1 g/L quercetin, and 2 mL/L Tween-80, with pH 5.5, inoculum size 9%, seed age 52 h, shaking speed 180 rpm, and fermentation culture 100 h, respectively. Furthermore, the addition of quercetin increased EPS production by 11.66%. The results also showed little citrinin residue in the EPS. The exopolysaccharides’ composition and antioxidant capacity of quercetin-modified exopolysaccharides were then preliminarily investigated. The addition of quercetin changed the composition of the exopolysaccharides and the molecular weight (Mw). In addition, the antioxidant activity of Monascus exopolysaccharides was monitored using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS+), and -OH. Monascus exopolysaccharides have good scavenging ability of DPPH and -OH. Furthermore, quercetin increased the scavenging ABTS+ ability. Overall, these findings provide a potential rationale for the application of quercetin in improving the EPS yield.

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Section: Effects Of Flavonoids On Crude Eps Yieldmentioning

confidence: 97%

Contribution of Quercetin to the Composition and Antioxidant Properties of Monascus Exopolysaccharides

Yang

Meng

Xie

et al. 2023

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“…A previous study by our group demonstrated that the addition of the exogenous substance genistein to Monascus purpureus, which is a long-established medical and edible fungus, could promote the secretion of one EPS, namely G-EMP (genistein-stimulated Monascus purpureus) . Although G-EMP exhibits excellent immune-modulatory properties in vitro through membrane TLR-4 activation of MAPK and NF-κB signaling pathways without toxicity, , its anti-inflammatory and immunomodulatory features in relation to ulcerative colitis are unknown. Additionally, previous studies have demonstrated that sustaining gut health relies on the interactions between the gut microbiota and inflammatory reactions of the host. , However, the role of gut microbiota in G-EMP-mediated IBD remission remains unknown.…”

Section: Introductionmentioning

confidence: 99%

An Exopolysaccharide from Genistein-Stimulated Monascus Purpureus: Structural Characterization and Protective Effects against DSS-Induced Intestinal Barrier Injury Associated with the Gut Microbiota-Modulated Short-Chain Fatty Acid-TLR4/MAPK/NF-κB Cascade Response

Xie,

Chen,

et al. 2024

J. Agric. Food Chem.

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Inflammatory bowel disease is a major health problem that can lead to prolonged damage to the digestive system. This study investigated the effects of an exopolysaccharide from genistein-stimulated Monascus purpureus (G-EMP) in a mouse model of colitis to clarify its molecular mechanisms and identified its structures. G-EMP (Mw = 56.4 kDa) was primarily consisted ofintervention reduced the loss of body weight, degree of colonic damage and shortening, disease activity index scores, and histopathology scores, while restoring goblet cell production and oxidative homeostasis, repairing colonic functions, and regulating inflammatory cytokines. RNA sequencing and Western blot analysis indicated that G-EMP exerts anti-inflammatory properties by suppressing the TLR4/MAPK/NF-κB inflammatory signaling pathway. G-EMP modulated the gut microbiota by improving its diversities, elevating the relative abundances of beneficial bacteria, declining the Firmicutes/Bacteroidota value, and regulating the level of short-chain fatty acids (SCFAs). Correlation analysis demonstrated strong links between SCFAs, gut microbiota, and the inflammatory response, indicating the potential of G-EMP to prevent colitis.

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“…Our previous study revealed that EPS secretion by M. purpureus 40,269 could be effectively promoted by adding the exogenous substance genistein, named G-EMP, with a 59% increase in yield . The average moisture and ash levels of G-EMP are 9.69 and 7.55%, respectively, consisting of arabinose, xylose, rhamnose, mannose, glucose, and galactose with molar ratios of approximately 1, 0.37, 0.14, 3.15, 4.15, and 5.04, respectively, and a relatively low molecular weight of 83.1 kDa .…”

Section: Introductionmentioning

confidence: 99%

“…The neutral sugar uronic contents and proteins of G-EMP were 61.77, 16.17, and 1.14%, respectively. Additionally, G-EMP is non-toxic and possesses strong anti-oxidant and immunomodulatory effects in vitro. , However, the potential mechanism by which G-EMP exerts the immunomodulatory effects in vivo and its relationship with intestinal flora are still unclear. Therefore, this study aimed to explore the actions of G-EMP in the intestinal immune response, oxidative stress, and gut microbiota of Cy-induced immunosuppressed mice.…”

Section: Introductionmentioning

confidence: 99%

Exopolysaccharides from Genistein-Stimulated Monascus purpureus Ameliorate Cyclophosphamide-Induced Intestinal Injury via PI3K/AKT-MAPKs/NF-κB Pathways and Regulation of Gut Microbiota

Xie,

Chen,

et al. 2023

J. Agric. Food Chem.

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Exopolysaccharides from genistein-stimulated Monascus purpureus (G-EMP) exhibited immunomodulatory potential in vitro, but whether it had immune-enhancing effects in vivo and its potential mechanism are not yet known. Here, the immunomodulatory effects of G-EMP were investigated by establishing an immunosuppressed mouse model treated with cyclophosphamide (Cy). The results suggested that G-EMP effectively alleviated the signs of weight reduction and diet reduction caused by Cy, increased fecal water content and splenic index, and decreased the oxidative stress of the liver. Simultaneously, G-EMP improved Cy-induced intestinal injury by restoring villus length, increasing the number of cupped cells, upregulating the expression of mucin and tight junction proteins, and downregulating the ratio of apoptotic proteins (Bax/Bcl-2). It also boosted the levels of mouse colonic cytokines, CD4 + and CD8 + T cells. Additionally, G-EMP markedly enhanced immunomodulation via the activation of PI3K/AKT-MAPKs/NF-κB signal pathways. Furthermore, G-EMP intervention displayed a positive association with most immunological indexes by elevating the levels of short-chain fatty acids, varying gut microbiota composition, and enhancing beneficial bacteria (Lactobacillaceae, Prevotellaceae, and S24-7). These findings demonstrated that G-EMP can strengthen immunity, repair intestinal mucosal damage, regulate gut microbiota, and be a potential source of prebiotics.

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Enhancement of functional activity and biosynthesis of exopolysaccharides in Monascus purpureus by genistein treatments

Cited by 5 publications

References 49 publications

Contribution of Quercetin to the Composition and Antioxidant Properties of Monascus Exopolysaccharides

Contribution of Quercetin to the Composition and Antioxidant Properties of Monascus Exopolysaccharides

An Exopolysaccharide from Genistein-Stimulated Monascus Purpureus: Structural Characterization and Protective Effects against DSS-Induced Intestinal Barrier Injury Associated with the Gut Microbiota-Modulated Short-Chain Fatty Acid-TLR4/MAPK/NF-κB Cascade Response

Exopolysaccharides from Genistein-Stimulated Monascus purpureus Ameliorate Cyclophosphamide-Induced Intestinal Injury via PI3K/AKT-MAPKs/NF-κB Pathways and Regulation of Gut Microbiota

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