Enhancement of gaseous o-xylene degradation in a microbial fuel cell by adding Shewanella oneidensis MR-1

You, Juping; Deng, Yingying; Chen, Han; Ye, Jiexu; Zhang, Shihan; Zhao, Jingkai

doi:10.1016/j.chemosphere.2020.126571

Cited by 42 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The 16S rRNA analysis in the present work revealed that the isolated bacterial species from anodic biofilm were 99.5–100% like certain strains of the following species: Acidovorax caeni , Acinetobacter haemolyticus , Delftia lacustris, and Pseudomonas geniculate . Pseudomonas (belonging to Gammaproteobacteria ) as the exoelectrogenic denitrifying bacterium, along with exoelectrogens including Delftia and Acinetobacter, contributed to the high performance on denitrification in denitrifying UTMFC and transferred electron extracellularly to solid electrode 20, 36, 48 . The clones similar to Acidovorax sp .…”

Section: Resultsmentioning

confidence: 99%

“…Pseudomonas (belonging to Gammaproteobacteria) as the exoelectrogenic denitrifying bacterium, along with exoelectrogens including Delftia and Acinetobacter, contributed to the high performance on denitrification in denitrifying UTMFC and transferred electron extracellularly to solid electrode. 20,36,48 The clones similar to Acidovorax sp. have been reported to be present in steroidal drug production wastewater (SPW)-fed anode.…”

Section: Effect Of External Resistance On Performance Of Utmfc At C/nmentioning

confidence: 99%

See 1 more Smart Citation

A novel process of anodic water denitrification in an upflow tubular microbial fuel cell: effect of C/N ratio

Davarpanah

Sarbisheh

Sharghi

2020

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: A high nitrate concentration in groundwater in some parts of Iran has been detected due to untreated wastewater discharge and agriculture activities. Therefore, the development of treatment technology in this area using a new biological method was carried out. RESULTS: Activated sludge originating from slaughterhouse wastewater treatment plants and immobilized on graphite granules exhibited noticeable denitrification activity in a double chamber upflow tubular microbial fuel cell (UTMFC). The effects of three C/N values at different initial nitrate concentrations and external resistances on nitrate and chemical oxygen demand (COD) removal efficiencies as well as cell performance were studied at a fixed hydraulic retention time (HRT) of 12 h. Based on nucleotide sequence of 16S rRNA, four bacterial strains from anodic biofilm were also identified. The UTMFC operated in a continuous mode with C/N values of 20 and 10 was able to denitrify up to 150 mg L-1 nitrate without more accumulation of nitrite and ammonium ions than is permitted for drinking water. The maximum nitrate removal capacity of 0.25 ± 0.01 kg NO 3 −-N/ (m 3 net anodic compartment 'NAC'.day) was achieved at 400 mg L-1 initial nitrate concentration and at C/N = 10. Additionally, the maximum current density and power generation at this ratio were found to be 46.04 A m-3 NAC and 16.96 W/m 3 NAC, respectively. No efficient denitrification was observed at C/N = 5 while nitrite/ammonium formation increased. CONCLUSION: The experimental data documented the feasibility of bioelectricity generation and substrate removal utilizing acetate containing water under the adopted operating conditions of UTMFC, and the proposed system allowed the simultaneous heterotrophic denitrification/electricity generation.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Effect Of External Resistance On Performance Of Utmfc At C/nmentioning

confidence: 99%

A novel process of anodic water denitrification in an upflow tubular microbial fuel cell: effect of C/N ratio

Davarpanah

Sarbisheh

Sharghi

2020

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…Some Shewanella sp. strains release volatile organic compounds as signals to mediate interactions between bacteria and their protist predators, , which might cause amoeba to eat B. laterosporus by mistake.…”

Section: Discussionmentioning

confidence: 99%

Soil Amoebae Affect Iron and Chromium Reduction through Preferential Predation between Two Metal-Reducing Bacteria

Huang

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

Soil protists are essential but often overlooked in soil and could impact microbially driven element cycling in natural ecosystems. However, how protists influence heavy metal cycling in soil remains poorly understood. In this study, we used a model protist, Dictyostelium discoideum, to explore the effect of interactions between soil amoeba and metal-reducing bacteria on the reduction of soil Fe(III) and Cr(VI). We found that D. discoideum could preferentially prey on the Fe(III)-reducing bacterium Shewanella decolorationis S12 and significantly decrease its biomass. Surprisingly, this predation pressure also stimulated the activity of a single S. decolorationis S12 bacterium to reduce Fe(III) by enhancing the content of electron-transfer protein cyt c, intracellular ATP synthesis, and reactive oxygen species (e.g., H2O2). We also found that D. discoideum could not prey on the Cr(VI)-reducing bacterium Brevibacillus laterosporus. In contrast, B. laterosporus became edible to amoebae in the presence of S. decolorationis S12, and their Cr(VI) reduction ability decreased under amoeba predation pressure. This study provides direct evidence that protists can affect the Cr and Fe cycling via the elective predation pressure on the metal-reducing bacteria, broadening our horizons of predation of protists on soil metal cycling.

show abstract

“…Bioelectrochemical systems (BESs) use the extracellular electron transfer (EET) of electroactive microorganisms (EAMs) to enable diverse applications in the fields of energy and environment, − including microbial fuel cells (MFCs) for bioelectricity harvesting and pollutant treating, − microbial electrosynthesis (MES) for the production of value-added chemicals and biofuels, − microbial electrolysis cells (MECs) for hydrogen production, − etc. The efficiency of EET is a key factor in determining the performance of BESs.…”

Section: Introductionmentioning

confidence: 99%

Modular Engineering Strategy to Redirect Electron Flux into the Electron-Transfer Chain for Enhancing Extracellular Electron Transfer in Shewanella oneidensis

et al. 2022

View full text Add to dashboard Cite

Efficient extracellular electron transfer (EET) of exoelectrogens is critical for practical applications of various bioelectrochemical systems. However, the low efficiency of electron transfer remains a major bottleneck. In this study, a modular engineering strategy, including broadening the sources of the intracellular electron pool, enhancing intracellular nicotinamide adenine dinucleotide (NADH) regeneration, and promoting electron release from electron pools, was developed to redirect electron flux into the electron transfer chain in Shewanella oneidensis MR-1. Among them, four genes include gene SO1522 encoding a lactate transporter for broadening the sources of the intracellular electron pool, gene gapA encoding a glyceraldehyde-3-phosphate dehydrogenase and gene mdh encoding a malate dehydrogenase in the central carbon metabolism for enhancing intracellular NADH regeneration, and gene ndh encoding NADH dehydrogenase on the inner membrane for releasing electrons from intracellular electron pools into the electron-transport chain. Upon assembly of the four genes, electron flux was directly redirected from the electron donor to the electron-transfer chain, achieving 62% increase in intracellular NADH levels, which resulted in a 3.5-fold enhancement in the power density from 59.5 ± 3.2 mW/m2 (wild type) to 270.0 ± 12.7 mW/m2 (recombinant strain). This study confirmed that redirecting electron flux from the electron donor to the electron-transfer chain is a viable approach to enhance the EET rate of S. oneidensis.

show abstract

Enhancement of gaseous o-xylene degradation in a microbial fuel cell by adding Shewanella oneidensis MR-1

Cited by 42 publications

References 42 publications

A novel process of anodic water denitrification in an upflow tubular microbial fuel cell: effect of C/N ratio

A novel process of anodic water denitrification in an upflow tubular microbial fuel cell: effect of C/N ratio

Soil Amoebae Affect Iron and Chromium Reduction through Preferential Predation between Two Metal-Reducing Bacteria

Modular Engineering Strategy to Redirect Electron Flux into the Electron-Transfer Chain for Enhancing Extracellular Electron Transfer in Shewanella oneidensis

Contact Info

Product

Resources

About