Enhancement of gentamicin production by mutagenesis and non-nutritional stress conditions in Micromonospora echinospora

Himabindu, M.; Potumarthi, Ravichandra; Jetty, Annapurna

doi:10.1016/j.procbio.2007.05.002

Cited by 17 publications

(14 citation statements)

References 16 publications

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“…No significant variation in OTC production was detected during 4 months corresponding to eight generations. This strain appeared genetically stable as previously described for gentamycin producing M. echinospora strain (Himabindu et al 2007). …”

Section: Resultsmentioning

confidence: 96%

Enhancement of oxytetracycline production after gamma irradiation-induced mutagenesis of Streptomyces rimosus CN08 strain

Lazim

Slama

Mankai

et al. 2010

World J Microbiol Biotechnol

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Streptomyces rimosus CN08 isolated from Tunisian soil produced 8.6 mg l(-1) of oxytetracycline (OTC) under submerged fermentation (SmF). Attempts were made for enhancing OTC production after irradiation-induced mutagenesis of Streptomyces rimosus CN08 with Co(60)-γ rays. 125 OTC-producing colonies were obtained after screening on kanamycin containing medium. One mutant called Streptomyces rimosus γ-45 whose OTC production increased 19-fold (165 mg l(-1)) versus wild-type strain was selected. γ-45 mutant was used for OTC production under solid-state fermentation (SSF). Wheat bran (WB) was used as solid substrate and process parameters influencing OTC production were optimized. Solid-state fermentation increased the yield of antibiotic production (257 mg g(-1)) when compared with submerged fermentation. Ammonium sulphate as additional nitrogen source enhanced OTC level to 298 mg g(-1). Interestingly, OTC production by γ-45 mutant was insensitive to phosphate which opens the way to high OTC production even in medium containing phosphate necessary for optimal mycelia growth.

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Section: Resultsmentioning

confidence: 96%

Enhancement of oxytetracycline production after gamma irradiation-induced mutagenesis of Streptomyces rimosus CN08 strain

Lazim

Slama

Mankai

et al. 2010

World J Microbiol Biotechnol

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“…Me-22 obtained by mutagenic treatment with Ethidium bromide [6] was used in the present study. Each 250 ml Erlenmeyer flasks contained 50 ml inoculum medium having composition (g l -1 ): beef extract, 3; glucose, 1; soluble starch, 24; yeast extract, 5; CaCO 3 , 4; pH 7.6 and inoculated with Me-22 (one slant for each flask) under aseptic conditions.…”

Section: Methodsmentioning

confidence: 99%

“…We have conducted subsequent experiments to produce mutant culture of M. echinospora by different methods [6] and found that M. echinospora EtBr-22 (Me-22) was producing the maximum gentamicin concentration in shake flask experiments using production medium as defined previously [5]. The purpose of the present study was to optimize the initial concentration of carbon and nitrogen sources to study kinetic aspects of Me-22 fermentation in stirred tank reactor (STR).…”

mentioning

confidence: 99%

Gentamicin production by Micromonospora echinospora (Me‐ 22) in stirred tank reactor: effect of various parameters

Meenavilli

Potumarthi

Jetty

2008

J. Basic Microbiol.

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Effect of production medium components, initial starch and soyabean meal concentrations, for the enhanced production of gentamicin by Micromonospora echinospora (Me- 22) was studied in a lab scale stirred tank reactor. Also effect of different aeration (0.5, 1, 2, and 4 vvm) and agitation rates (100, 200, 300 and 400 rpm) in a stirred tank reactor was examined. A maximum gentamicin concentration of 2.68 g l(-1) was achieved in the medium having low concentrations of initial starch (7.5 g l(-1)) and high concentrations of initial soyabean meal (4 g l(-1)). Both aeration and agitation significantly affected gentamicin concentration, productivity and biomass formation. The maximum gentamicin concentration of 4.12 g l(-1) and the highest yield of gentamicin on substrate 0.967 g g(-1) were obtained at impeller speed of 200 rpm and aeration rate of 2 vvm. Under optimal culture conditions in STR the production of gentamicin could be increased 3 fold when compared with shake flask.

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“…Microbial process development across the globe is increasing because of its low energy and materials consuming and eco‐friendly approach. Various microbial products have been produced by aerobic fermentations includes both primary and secondary metabolites . Antibiotics, enzymes and demand for microbial enzymes are increasing at the rate of 6.3% of per annum and are expected to reach 70 billion USD by 2013, and the rationale for such demand is due to their eco‐friendly nature for process and product development.…”

Section: Introductionmentioning

confidence: 99%

Surface immobilization of Rhizopus oryzae (ATCC 96382) for enhanced production of lipase enzyme by multiple responses optimization

Potumarthi

Liu

Wan

et al. 2012

Asia-Pacific J Chem Eng

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A modified Response Surface Methodology was used for the optimization of lipase production by immobilized cells of Rhizopus oryzae (ATCC 96382). Multiple responses optimization strategy was adopted to optimize process condition of surface immobilization technique for the optimization of lipase productivity. Five parameters, pH, Temperature, revolutions per minute, olive oil and glucose were considered, and their effect was evaluated both qualitatively and quantitatively by using a modified central composite design approach and model validation. The second order regression equation was developed for the three yield parameters are YP/B, YL/P and YL/B, where Y is the yield parameter at their corresponding values of L: Lipase (U/mL), P: Protein (mg/mL) and B: Biomass (mg/mL). The regression equation was fitted and model fit (R2) was 0.604 (YP/B), 0.746 (YL/P) and 0.618 (YL/B), respectively showing an effective fit. Further, the model was validated theoretically and experimentally at higher level of production. Different growth patterns were observed during 120 h of fermentation period. © 2012 Curtin University of Technology and John Wiley & Sons, Ltd.

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Enhancement of gentamicin production by mutagenesis and non-nutritional stress conditions in Micromonospora echinospora

Cited by 17 publications

References 16 publications

Enhancement of oxytetracycline production after gamma irradiation-induced mutagenesis of Streptomyces rimosus CN08 strain

Enhancement of oxytetracycline production after gamma irradiation-induced mutagenesis of Streptomyces rimosus CN08 strain

Gentamicin production by Micromonospora echinospora (Me‐ 22) in stirred tank reactor: effect of various parameters

Surface immobilization of Rhizopus oryzae (ATCC 96382) for enhanced production of lipase enzyme by multiple responses optimization

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