Enhancement of gibberellic acid production from Fusarium fujikuroi by mutation breeding and glycerol addition

Peng, Xiao-Lun; Zhao, Wei; Wang, Yuan-Shan; Dai, Ke-Lei; Cen, Yu-Ke; Liu, Zhi‐Qiang; Zheng, Yu‐Guo

doi:10.1007/s13205-020-02303-4

Cited by 8 publications

(7 citation statements)

References 47 publications

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“…Similarly combined mutagenesis using both nitrosoguanidine and 60 Co gamma rays, along with screening for resistance against the fungicide terbinafine resulted in a 11.87% increase of the GA3 titer in the best strain (Wang et al 2014 ). ARTP caused DNA damage by chemical active particles with the advantages of lower capital costs, higher mutation rates, and low treatment temperatures compared with traditional UV and chemical mutagenesis methods (Lu et al 2011 ; Peng et al 2020 ). As reported, ARTP has already applied for obtaining dominant positive mutants from bacteria, fungi, and microalgae (Zhang et al 2015 ), which is a potential method for increasing GA3 titer in F. fujikuroi .…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Yang

Peng

et al. 2022

Bioresour. Bioprocess.

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Gibberellic acid (GA3) is a plant growth hormone that plays an important role in the production of crops, fruits, and vegetables with a wide market share. Due to intrinsic advantages, liquid fermentation of Fusarium fujikuroi has become the sole method for industrial GA3 production, but the broader application of GA3 is hindered by low titer. In this study, we combined atmospheric and room-temperature plasma (ARTP) with ketoconazole-based screening to obtain the mutant strain 3-6-1 with high yield of GA3. Subsequently, the medium composition and fermentation parameters were systematically optimized to increase the titer of GA3, resulting in a 2.5-fold increase compared with the titer obtained under the initial conditions. Finally, considering that the strain is prone to substrate inhibition and glucose repression, a new strategy of fed-batch fermentation was adopted to increase the titer of GA3 to 575.13 mg/L, which was 13.86% higher than the control. The strategy of random mutagenesis combined with selection and fermentation optimization developed in this study provides a basis for subsequent research on the industrial production of GA3. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Yang

Peng

et al. 2022

Bioresour. Bioprocess.

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“…The content of icariin was analyzed using a HPLC system. In order to increase enzyme activity, various intensities of gamma rays (60 Co γ , 250 Gy, 500 Gy, 750 Gy, 1000 Gy) and different concentrations of nitrosoguanidine (0.5, 1, 1.5, 2 M) were applied to mutagenesis of the target strain [ 21 ]. After mutagenesis, Luria Bertani (LB) medium including pNPR was used on the first screening.…”

Section: Methodsmentioning

confidence: 99%

Purification and Characterization of a Novel α-L-Rhamnosidase from Papiliotrema laurentii ZJU-L07 and Its Application in Production of Icariin from Epimedin C

Lou

Liu

et al. 2022

JoF

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Icariin is the most effective bioactive compound in Herba Epimedii. To enhance the content of icariin in the epimedium water extract, a novel strain, Papiliotrema laurentii ZJU-L07, producing an intracellular α-L-rhamnosidase was isolated from the soil and mutagenized. The specific activity of α-L-rhamnosidase was 29.89 U·mg−1 through purification, and the molecular mass of the enzyme was 100 kDa, as assayed by SDS-PAGE. The characterization of the purified enzyme was determined. The optimal temperature and pH were 55 °C and 7.0, respectively. The enzyme was stable in the pH range 5.5–9.0 for 2 h over 80% and the temperature range 30–40 °C for 2 h more than 70%. The enzyme activity was inhibited by Ca2+, Fe2+, Cu2+, and Mg2+, especially Fe2+. The kinetic parameters of Km and Vmax were 1.38 mM and 24.64 μmol·mg−1·min−1 using pNPR as the substrate, respectively. When epimedin C was used as a nature substrate to determine the kinetic parameters of α-L-rhamnosidase, the values of Km and Vmax were 3.28 mM and 0.01 μmol·mg−1·min−1, respectively. The conditions of enzymatic hydrolysis were optimized through single factor experiments and response surface methodology. The icariin yield increased from 61% to over 83% after optimization. The enzymatic hydrolysis method could be used for the industrialized production of icariin. At the same time, this enzyme could also cleave the α-1,2 glycosidic linkage between glucoside and rhamnoside in naringin and neohesperidin, which could be applicable in other biotechnological processes.

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“…This available information has allowed that different works can be focused on the use of raw materials with the purpose of solving two problems, the increase of GA 3 production and the waste utilization of residues from different agro-industries. The most employed raw materials are starch [ 68 ], corn flour [ 69 ], wheat bran [ 70 ], rice bran [ 7 ], barley flour, citrus pulp [ 71 ], corn starch [ 72 ], rice flour [ 8 ], and soybean flour [ 73 ], among others.…”

Section: Ga 3 Productionmentioning

confidence: 99%

“…Many research works have suggested a variety of strategies to optimize production, with the most common being the optimization of growth parameters such as incubation time, cultivation temperature, pH, humidity, and water activity (the last two for SSF), as is analyzed in Table 1 . On the other hand, further research suggests a broader range of approaches, including coupling extraction during fermentation [ 74 , 75 ], the use of mutant strains [ 69 , 72 ], models and simulations [ 76 , 77 ], pH and temperature regulation [ 61 , 65 ], and the testing of different carbon–nitrogen ratios (C:N) [ 8 , 78 ]. Next, we discuss the relationship between the GA 3 concentration reached when using raw materials and the incubation conditions; the most interesting reports of GA 3 production are described from a process point of view.…”

Section: Ga 3 Productionmentioning

confidence: 99%

Bioprocess of Gibberellic Acid by Fusarium fujikuroi: The Challenge of Regulation, Raw Materials, and Product Yields

Hernández Rodríguez,

Díaz Pacheco,

Martínez Tolibia

et al. 2024

JoF

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Gibberellic acid (GA3) is a tetracyclic diterpenoid carboxylic acid synthesized by the secondary metabolism of Fusarium fujikuroi. This phytohormone is widely studied due to the advantages it offers as a plant growth regulator, such as growth stimulation, senescence delay, flowering induction, increased fruit size, and defense against abiotic or biotic stress, which improve the quality and yield of crops. Therefore, GA3 has been considered as an innovative strategy to improve agricultural production. However, the yields obtained at large scale are insufficient for the current market demand. This low productivity is attributed to the lack of adequate parameters to optimize the fermentation process, as well as the complexity of its regulation. Therefore, this article describes the latest advances for potentializing the GA3 production process, including an analysis of its origins from crops, the benefits of its application, the related biosynthetic metabolism, the maximum yields achieved from production processes, and their association with genetic engineering techniques for GA3 producers. This work provides a new perspective on the critical points of the production process, in order to overcome the limits surrounding this modern line of bioengineering.

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Enhancement of gibberellic acid production from Fusarium fujikuroi by mutation breeding and glycerol addition

Cited by 8 publications

References 47 publications

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Purification and Characterization of a Novel α-L-Rhamnosidase from Papiliotrema laurentii ZJU-L07 and Its Application in Production of Icariin from Epimedin C

Bioprocess of Gibberellic Acid by Fusarium fujikuroi: The Challenge of Regulation, Raw Materials, and Product Yields

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