Enhancement of human bladder carcinoma cell chemosensitivity to Mitomycin C through quasi-monochromatic blue light (λ = 453 ± 10 nm)

Hegmann, Lisa; Sturm, Sofia; Niegisch, Günter; Windolf, Joachim; Suschek, Christoph V.

doi:10.1016/j.jphotobiol.2022.112582

Cited by 4 publications

(7 citation statements)

References 53 publications

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“…The result of this high ROS load on cells might be the loss of integrity and function of nucleic acids, proteins and membranes. As we were able to show earlier [35], the latter point in particular can lead to the escape of cytochrome C into the cytosol and thus to the induction of apoptotic cell death by disrupting the membrane function of mitochondria. The molecular mechanism of apoptosis is fine-tuned and programmed by the cell.…”

Section: Discussionsupporting

confidence: 51%

“…Recently, we were able to show that blue light with a wavelength of 453 nm, used in non-toxic doses, significantly increases the cytotoxic potential of mitomycin C against the human bladder cell carcinoma cell line RT-112. In that study, light-modulated increase in cytotoxicity, mainly registered in the form of apoptosis and secondary necrosis, correlated with a significant increase in intracellular production of ROS and a decrease in mitochondrial respiration [35].…”

Section: Discussionmentioning

confidence: 79%

“…Blue light has already been evaluated in cancer treatment of fibrosarcoma [24], leukaemia [25], melanoma [26,27], skin tumour [28] and colon cancer [24,29] and is used in the treatment of acne [30], actinic keratoses [31], atopic dermatitis [32], plaque psoriasis [33] and icterus neonatorum [34]. Very recently, we could demonstrate that blue light exposure (453 nm) of human urothelial bladder carcinoma cells notably enhanced the therapeutic efficiency of mitomycin C in the form of significantly enhanced cytotoxicity via apoptosis and secondary necrosis [35]. Due to these properties, blue light might represent an effective, low-side-effect and successenhancing therapy option in the local treatment of bladder cancer.…”

Section: Of 19mentioning

confidence: 99%

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Exposure of Bladder Cancer Cells to Blue Light (λ = 453 nm) in the Presence of Riboflavin Synergistically Enhances the Cytotoxic Efficiency of Gemcitabine

Sturm,

Niegisch,

Windolf

et al. 2024

IJMS

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Non-muscle invasive bladder cancer is a common tumour in men and women. In case of resistance to the standard therapeutic agents, gemcitabine can be used as off-label instillation therapy into the bladder. To reduce potential side effects, continuous efforts are made to optimise the therapeutic potential of drugs, thereby reducing the effective dose and consequently the pharmacological burden of the medication. We recently demonstrated that it is possible to significantly increase the therapeutic efficacy of mitomycin C against a bladder carcinoma cell line by exposure to non-toxic doses of blue light (453 nm). In the present study, we investigated whether the therapeutically supportive effect of blue light can be further enhanced by the additional use of the wavelength-specific photosensitiser riboflavin. We found that the gemcitabine-induced cytotoxicity of bladder cancer cell lines (BFTC-905, SW-1710, RT-112) was significantly enhanced by non-toxic doses of blue light in the presence of riboflavin. Enhanced cytotoxicity correlated with decreased levels of mitochondrial ATP synthesis and increased lipid peroxidation was most likely the result of increased oxidative stress. Due to these properties, blue light in combination with riboflavin could represent an effective therapy option with few side effects and increase the success of local treatment of bladder cancer, whereby the dose of the chemotherapeutic agent used and thus the chemical load could be significantly reduced with similar or improved therapeutic success.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 79%

Section: Of 19mentioning

confidence: 99%

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Exposure of Bladder Cancer Cells to Blue Light (λ = 453 nm) in the Presence of Riboflavin Synergistically Enhances the Cytotoxic Efficiency of Gemcitabine

Sturm,

Niegisch,

Windolf

et al. 2024

IJMS

Self Cite

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show abstract

“…We used three vital dyes to detect and quantify cytotoxic events as described previously [72,73]. Vital cells were detected using fluorescein diacetate (FDA), apoptotic cells were visualized and quantified using Hoechst 33342 dye and we used propidium iodide to detect and quantify necrotic events.…”

Section: Toxicity Viability and Proliferationmentioning

confidence: 99%

“…The experiments were evaluated using a fluorescence microscope (Zeiss, Wetzlar, Germany). In addition, we characterized the vitality and cell number in the respective differently treated cell cultures using a resazurin-based assay (CellTiter-Blue, Promega, Madison, WI, USA) and using a fluorescence spectrometer (VICTOR II Plate Reader, PerkinElmer, Waltham, MA, USA) at an excitation wavelength of 540 nm and an emission wavelength of 590 nm, as described previously [72,73].…”

Section: Toxicity Viability and Proliferationmentioning

confidence: 99%

Gas Flow-Dependent Modification of Plasma Chemistry in μAPP Jet-Generated Cold Atmospheric Plasma and Its Impact on Human Skin Fibroblasts

et al. 2023

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The micro-scaled Atmospheric Pressure Plasma Jet (µAPPJ) is operated with low carrier gas flows (0.25–1.4 slm), preventing excessive dehydration and osmotic effects in the exposed area. A higher yield of reactive oxygen or nitrogen species (ROS or RNS) in the µAAPJ-generated plasmas (CAP) was achieved, due to atmospheric impurities in the working gas. With CAPs generated at different gas flows, we characterized their impact on physical/chemical changes of buffers and on biological parameters of human skin fibroblasts (hsFB). CAP treatments of buffer at 0.25 slm led to increased concentrations of nitrate (~352 µM), hydrogen peroxide (H2O2; ~124 µM) and nitrite (~161 µM). With 1.40 slm, significantly lower concentrations of nitrate (~10 µM) and nitrite (~44 µM) but a strongly increased H2O2 concentration (~1265 µM) was achieved. CAP-induced toxicity of hsFB cultures correlated with the accumulated H2O2 concentrations (20% at 0.25 slm vs. ~49% at 1.40 slm). Adverse biological consequences of CAP exposure could be reversed by exogenously applied catalase. Due to the possibility of being able to influence the plasma chemistry solely by modulating the gas flow, the therapeutic use of the µAPPJ represents an interesting option for clinical use.

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ARHGAP6 inhibits bladder cancer cell viability, migration, and invasion via β-catenin signaling and enhances mitomycin C sensitivity

Tan

Lü

et al. 2023

Human Cell

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Enhancement of human bladder carcinoma cell chemosensitivity to Mitomycin C through quasi-monochromatic blue light (λ = 453 ± 10 nm)

Cited by 4 publications

References 53 publications

Exposure of Bladder Cancer Cells to Blue Light (λ = 453 nm) in the Presence of Riboflavin Synergistically Enhances the Cytotoxic Efficiency of Gemcitabine

Exposure of Bladder Cancer Cells to Blue Light (λ = 453 nm) in the Presence of Riboflavin Synergistically Enhances the Cytotoxic Efficiency of Gemcitabine

Gas Flow-Dependent Modification of Plasma Chemistry in μAPP Jet-Generated Cold Atmospheric Plasma and Its Impact on Human Skin Fibroblasts

ARHGAP6 inhibits bladder cancer cell viability, migration, and invasion via β-catenin signaling and enhances mitomycin C sensitivity

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