Enhancement of IgE synthesis and histamine release by T cell factors derived from atopic patients with bronchial asthma

Yanagihara, Yukiyoshi; Kondo, Keiichi; Kiniwa, Mamoru; Yui, Yasuo; Shida, Takao; Delespesse, Guy

doi:10.1016/0091-6749(87)90362-9

Cited by 20 publications

(24 citation statements)

References 26 publications

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“…To estimate the passive release of preformed IgE and IgG into the culture supernatant, both cycloheximide and puromycin were added to some cultures (10,11). After 10 days, the culture supernatant was pooled from three wells, and IgE and IgG concentrations were measured by a solid-phase sandwich radioimmunoassay (RIA) as previ ously described (10,11).…”

Section: Isolation Of Allergen-specific Helper Tcl (Sn-4)mentioning

confidence: 99%

“…Human peripheral blood mononuclear cells (PBMC) were isolated from heparinized venous blood by centrifu gation on a Ficoll-sodium metrizoate gradient, and T and B cell fractions were separated from PBMC by rosetting with AET-treated sheep erythrocytes, as previously described (10,11). To obtain purified T cells, rosetting cells were incubated with anti-CD21 and anti-CD 11 b mAbs and rabbit complement; they contained neither CD20+ B cells nor CD 14+ monocytes as analyzed by flow cytometry (FACS 440, Becton Dickinson) equipped with argon laser running at 488 nm.…”

Section: Cell Preparationmentioning

confidence: 99%

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Suppression of IgE Production by IPD-1151T (Suplatast Tosilate), a New Dimethylsulfonium Agent: (2) Regulation of Human IgE Response

Yanagihara

Kiniwa

Ikizawa

et al. 1993

Japanese Journal of Pharmacology

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ABSTRACT-The ability of IPD-1151T to suppress the induction of human IgE synthesis was investigated with an in vitro model of IgE production mediated by an allergen-specific helper T cell line (SN-4) from a patient allergic to Japanese cedar pollen. IPD-1151T induced a concentration-dependent suppression of purified allergen (Cry j 1)-dependent IgE synthesis in autologous B cell cultures mediated by SN-4, without significantly affecting the IgG synthesis. In addition, the production of interleukin 4 (IL-4) by Cry j 1-ac tivated SN-4 as well as that by phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC) of normal donors was inhibited in a concentration-dependent manner by the agent. Interestingly, IPD-1151T clearly depressed PHA-induced expression of IL-4 mRNA in normal PBMC, indicating that this agent inhibits IL-4 gene transcription. However, IPD-1151T had no antagonistic action on IL-4, since neither IL-4-induced expression of low-affinity IgE receptor (Fc e RII/CD23) on normal B cells nor soluble Fc e RII release from IL-4-stimulated B cells was affected by the agent. On the other hand, IPD-1151T had no effect on the production of interferon-y by both Cry j 1-stimulated SN-4 and anti-CD3 monoclonal anti body-activated T cells of normal donors. These results suggest that the selective suppression of IgE syn thesis by IPD-1151T results from the inhibition of IL-4 production by T cells at the gene level.

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Section: Isolation Of Allergen-specific Helper Tcl (Sn-4)mentioning

confidence: 99%

Section: Cell Preparationmentioning

confidence: 99%

Suppression of IgE Production by IPD-1151T (Suplatast Tosilate), a New Dimethylsulfonium Agent: (2) Regulation of Human IgE Response

Yanagihara

Kiniwa

Ikizawa

et al. 1993

Japanese Journal of Pharmacology

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“…To determine IgE levels in the culture supernatants containing rsFcERIa, rsFccRIa at a final concentration of 100 ng/ml was usually added to the control culture supernatant before the assay. IgG, IgM, and IgA concentrations in the culture supernatants were measured by sandwich RIAs exactly as described (10), and each assay was not affected by adding rsFccRIa at any concentration.…”

Section: Methodsmentioning

confidence: 99%

Recombinant soluble form of the human high-affinity immunoglobulin E (IgE) receptor inhibits IgE production through its specific binding to IgE-bearing B cells.

Yanagihara

Kondo²,

Ikizawa³

et al. 1994

J. Clin. Invest.

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A recombinant soluble form of the a subunit of the human high-affinity receptor for IgE (rsFceRla), one of the potent IgE-binding molecules, was tested for its ability to regulate IL-4-induced IgE synthesis by human lymphocytes. Addition of rsFcERIc to cultures induced a dose-dependent inhibition of the T cell-dependent and independent synthesis of IgE. The suppression of IgE synthesis was observed at the protein and the mRNA levels, and it was IgE class specific. By flow cytometry, specific binding of rsFceRlei was detected on surface IgE-bearing B cells as well as on U266 cells, and it was completely blocked by preincubation with IgE. rsFcERIac bound to the cell surface IgE could be effectively dissociated not only by a large excess of IgE, but also by an anti-rsFceRfle mAb that competes with IgE for the binding to rsFceRla. This mAb abolished the rsFcERIamediated suppression of IgE synthesis. These data suggest that rsFceRIa may have a function in selectively suppressing IgE synthesis through its interaction with the membrane-bound form of IgE. (J. Clin. Invest. 1994. 94:2162-2165

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“…IgE and IgG concentrations in the culture supernatants were determined after culture for 14 days by means of solid-phase sandwich radioimmunoassays (RIAs) as previously described [24]. Control cultures for the evaluation of preformed immunoglobulin were carried out in the presence of both cycloheximide and puromycin as described [24]. After culture supernatants for the measurement of IgE and IgG were collected, the number of viable cells was evaluated by MTT assay [25].…”

Section: Cetl Preparations and Culture Condition'dmentioning

confidence: 99%

“…Incorporated radioactivity was measured by a liquid scintillation counter. IgE and IgG concentrations in the culture supernatants were determined after culture for 14 days by means of solid-phase sandwich radioimmunoassays (RIAs) as previously described [24]. Control cultures for the evaluation of preformed immunoglobulin were carried out in the presence of both cycloheximide and puromycin as described [24].…”

Section: Cetl Preparations and Culture Condition'dmentioning

confidence: 99%

Inhibition of IL-4 receptor up-regulation on B cells by antisense oligodeoxynucleotide suppresses IL-4-induced human IgE production

Ikizawa

Kondo

Koshio

et al. 1995

Clinical and Experimental Immunology

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SUMMARYlL-4 is shown to up-regulate its own receptor (IL-4R) on human lymphocytes, but the functional significance of up-regulated IL-4R is not clear regarding IgE production. This study investigated the possible role of IL-4-induced up-regulation of IL-4R on B cells in the induction ofhuman IgE synthesis by means of antisense strategy. Among three antisense oligodeoxynucleotides designed against the downstream of translation initiation siteof 1L-4R cDNA. S-oligo [.complementary to nucleotide 1-24, showed the strongest inhibition ofthe constitutive expression of IL-4R on Daudi cells. Addition of S-oligo ! together with IL-4 also decreased the up-regulated but not constitutive levels of IL-4R on peripheral blood B cells without affecting the concomitant enhancement of CD23, CD40, HLA-DR and surface IgM expression, indicating that ils effect is specific for IL-4R up-regulation. When S-oligo 1 was added to B cells costimulated with IL-4 and anti-CD40 MoAb, it induced a dose-dependent inhibition of IgE production. This inhibition was accompanied by a decrease in the expression of mature C< transcripts, whereas the accumulation of germ-line O transcripts was not affected by S-oligo 1. These data suggest that the signal transduclion mediated by the up-regulated IL-4R on B cells may be intimately associated with the induction of isotype switching to IgE thai leads to mature Ce transcription and IgE production.

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Enhancement of IgE synthesis and histamine release by T cell factors derived from atopic patients with bronchial asthma

Cited by 20 publications

References 26 publications

Suppression of IgE Production by IPD-1151T (Suplatast Tosilate), a New Dimethylsulfonium Agent: (2) Regulation of Human IgE Response

Suppression of IgE Production by IPD-1151T (Suplatast Tosilate), a New Dimethylsulfonium Agent: (2) Regulation of Human IgE Response

Recombinant soluble form of the human high-affinity immunoglobulin E (IgE) receptor inhibits IgE production through its specific binding to IgE-bearing B cells.

Inhibition of IL-4 receptor up-regulation on B cells by antisense oligodeoxynucleotide suppresses IL-4-induced human IgE production

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