Enhancement of Immune Response in Peyer's Patch Cells Cultured with Bifidobacterium breve

Abstract

Bifidobacterium breve, included in fermented milk, was tested for adjuvanticity and mitogenicity using cells of mouse Peyer's patch, one of the gut-associated lymphoid tissues. Addition of B. breve enhanced antilipopolysaccharide antibody production in Peyer's patch cells and also anti-sheep red blood cell plaque-forming cells in Peyer's patch cells cultured with sheep red blood cells. Furthermore, addition of B. breve accelerated proliferation of Peyer's patch cells, particularly B cells. In BALB/c mice, enhancement of proliferation by B. breve was also found in Peyer's patch cells from nude mice and a B cell-enriched fraction, including both the B cell fraction and plastic-adherent cells. Enhancement was not found in the fraction in which Sephadex G10-adherent and carbonyl-iron phagocytic cells were excluded from Peyer's patch cells or in a pure B cell fraction in which plastic-adherent cells were excluded from the B cell-enriched fraction of Peyer's patch cells. The proliferation of B cells was enhanced when the supernatant of plastic-adherent cells cultured with B. breve was added. It is concluded that B. breve activated plastic-adherent cells and that these cells secreted a soluble factor that enhanced proliferation of B cells.

Effect of Dietary Inulin Source on Piglet Performance, Immunoglobulin Concentration, and Plasma Lipid Profile

“…They can directly improve health status through stimulation of the activity of phagocytic cells as well as nonspecific humoral immunity mechanisms. The studies of other authors (9,16,18,26) showed that prebiotic supplementation, including inulin, enhances synthesis and secretory activity of IgA in the gastrointestinal tract. The present research included the analysis of the concentration of immunoglobulins class A, G, and M in piglet blood plasma.…”

“…Besides, inulin contributes to the induction of immunoglobulin synthesis, especially IgA (16,26). Inulin fructooligosaccharides induce proliferation of beneficial microflora (Bifiobacterium and Lactobacteriaceae), which as probiotic bacteria improve animal rearing performance (1,4).…”

Bifidobacterium adolescentis as a delivery system of endostatin for cancer gene therapy: Selective inhibitor of angiogenesis and hypoxic tumor growth

“…It can specifically reach tumor tissue by circulation and grow in hypoxic solid tumors. Bifidobacterium is beneficial rather than nonpathogenic for its host, immunobiologic, 16,21 antiinfective, 22 and antiaging, 23,24 and observably inhibits the growth of tumors in mice. 25 Therefore, with Bifidobacterium as a vector for gene therapy, its inherent role as antitumor makes it more effective in inhibition of tumor growth.…”

“…There are several advantages of selecting Bifidobacterium as gene delivery vector for cancer gene therapy: (1 ) Bifidobacterium is a domestic anaerobic bacterium in the human body that does not produce endotoxin and toxin; (2 ) Bifidobacterium increases immune response 16 and inhibits many tumor growth in vivo such as liver cancer, breast cancer, etc. ; 17 (3) Bifidobacterium can be killed easily by antibiotics or in oxygen environment in vitro or in vivo.…”

Bifidobacterium longum as a delivery system of TRAIL and endostatin cooperates with chemotherapeutic drugs to inhibit hypoxic tumor growth

“…[9][10][11] In addition, combination treatment with TRAIL and chemotherapy showed a synergistic effect in human cancer cell lines and tumor xenograft models, even in drug-resistant cells and tumors. [12][13][14] Not only as probiotics but also as anaerobe, Bifidobacterium longum can be used as a useful host cell for heterologous protein production due to the following reasons: (1) protection of the intestinal mucosa and suppression of the permanent planting of detrimental gut microorganisms; 15,16 (2) induction of the releasing of endogenous mediator with immunological activity and enhancement of immunity of host 17 and (3) inhibition of tumor growth by suppressing cancer related genes, selective localization and proliferation within hypoxic tumors. [18][19][20][21][22] However, the fact that exogenous plasmids cannot replicate stably in Bifidobacterium limits the application of Bifidobacterium in cancer gene therapy.…”