Enhancement of immunostimulation and anti-metastasis in submerged culture of bearded tooth mushroom (Hericium erinaceum) mycelia by addition of ginseng extract

Immunostimulatory Effect of Postbiotics Prepared from Phellinus linteus Mycelial Submerged Culture via Activation of Spleen and Peyer’s Patch in C3H/HeN Mice

“…After euthanasia, splenocytes were isolated from the spleen tissue of all the mice according to a previously described method [ 8 ], and the cell numbers were adjusted to a density of 2.5 × 10 6 cells/mL. The cell suspension (200 μL) was plated in a 96-well cell culture plate (Corning Costar Corp., Cambridge, MA, USA) and incubated in a humidified incubator maintained at 95% air, 5% CO 2 .…”

“…Recently, several medicinal mushrooms have been considered as important natural resources to prevent and/or treat various types of human diseases [ 3 ]. Although almost all parts of mushrooms, such as fruiting bodies, sclerotia, mycelia, and spores, have been used for medicinal or health promoting purposes [ 4 , 5 ], in recent years, the submerged culture-based mycelium production and use of the whole culture broth (as a source of postbiotics) have been increasing in various industrial fields including health functional foods, cosmeceuticals, and pharmaceuticals, because of their various advantages, such as low production costs, sustainable and uniform productivity, and eco-friendliness [ 6 , 7 , 8 ]. Moreover, a considerable number of novel biological metabolites could be produced during mushroom mycelium cultivation process using the submerged culture [ 9 ].…”

In vitro assessment of the gastrointestinal tolerance and immunomodulatory function of Bacillus methylotrophicus isolated from a traditional Korean fermented soybean food

“…Peyer's patch (PP) cells were prepared as described previously (Kim et al . ; Hwang et al . ), with slight modifications.…”

“…; Kim et al . ; Thomas et al . ), 6‐week‐old five ICR mice were intraperitoneally administered with 2 ml of 3% thioglycollate medium (BD Diagnostic Systems, Sparks, MD).…”

Polysaccharide from Hericium erinaceus improved laying performance of aged hens by promoting yolk precursor synthesis and follicle development via liver-blood-ovary axis

“…( Rodrigues et al, 2015 ). Previous studies have shown that HEP has immunomodulatory ( Kim et al, 2010 ), antioxidant activity ( Zhang et al, 2012 ), hepatoprotective effect ( Cui et al, 2016 ), anti-hyperglycemic, and hypolipidemic properties ( Yang et al, 2003 ; Nie et al, 2019 ). The application of HEP in livestock and aquaculture has been reported previously.…”