Enhancement of Intracellular Delivery of CdTe Quantum Dots (QDs) to Living Cells by Tat Conjugation

Xue, Fengfeng; Chen, J. Y.; Guo, Jia; Wang, C. C.; Yang, Wuli; Wang, P. N.; Lu, Daru

doi:10.1007/s10895-006-0152-2

Cited by 59 publications

(24 citation statements)

References 27 publications

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“…Upon surface modifications, solubility of QDs can be improved making them suitable for biological applications. Further, conjugation with CPPs facilitates cellular uptake of QDs [21,27,51,52]. Though CPPconjugated QDs raise the efficiency of cellular internalization, intracellular distributions of CPP-conjugated QDs have not yet been closely examined.…”

Section: Discussionmentioning

confidence: 99%

Intracellular delivery of quantum dots mediated by a histidine- and arginine-rich HR9 cell-penetrating peptide through the direct membrane translocation mechanism

et al. 2011

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Section: Discussionmentioning

confidence: 99%

Intracellular delivery of quantum dots mediated by a histidine- and arginine-rich HR9 cell-penetrating peptide through the direct membrane translocation mechanism

et al. 2011

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“…Previous studies have demonstrated that the modification of the surface of QDs with CPPs improves the labeling rate of cells (37,38). Xue et al (39) developed sulfhydryl-modified QDs combined with the TAT protein to obtain TAT-QD compounds. The QGY liver cancer and the MCF-7 human breast cancer cell lines were cultivated with TAT-QDs and these cells were assessed by laser confocal scanning microscopy (39).…”

Section: Discussionmentioning

confidence: 99%

“…Xue et al (39) developed sulfhydryl-modified QDs combined with the TAT protein to obtain TAT-QD compounds. The QGY liver cancer and the MCF-7 human breast cancer cell lines were cultivated with TAT-QDs and these cells were assessed by laser confocal scanning microscopy (39). The penetration of TAT-QDs into the QGY and MCF-7 cells was observed to increase compared with unmodified QDs, by 2.1 and 1.5 times, respectively.…”

Section: Discussionmentioning

confidence: 99%

Effect of quantum dots on the biological behavior of the EJ human bladder urothelial carcinoma cell line

Yuan

Zhang

et al. 2015

Molecular Medicine Reports

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Abstract. Quantum dots (QDs) are a type of fluorescent label with applications in biological molecules, cells and in vivo imaging. The current study investigated the effect of QDs on the toxicity, proliferation, migration and invasion of the EJ human bladder cancer cell line in vitro. The cell counting kit-8 test was used to measure the survival rate of EJ cells following incubation with varying concentrations of QDs. Additionally, the effect of QDs on tumor cell migration and invasion was evaluated using the Transwell chamber assay, and cell proliferation rate was assessed using a hemocytometer. Data from the current study demonstrated no significant differences in survival rate between the experimental and control groups with the conventionally used concentrations (5, 10 and 20 nM) of QD605 (P>0.05). However, with high concentrations of QD605 (40 and 80 nM), significant differences were observed (P<0.001). The survival rate of EJ cells, however, remained at 92.6%. In addition, no significant differences were observed between the EJ cells labeled with transactivator of transcription (TAT)-QD605 and the unlabeled EJ cells with regard to proliferation, migration and invasion (P>0.05). Thus, the results of the current study indicate that QDs exhibit a certain degree of influence on the activity of the EJ bladder cancer cell line at high concentrations. However, at the concentrations that QDs are conventionally used, there was little impact on the survival of the EJ cells. In addition, the proliferation, migration and invasion abilities of the EJ cells were not affected by TAT-QDs. Therefore, the peptide-conjugated QDs have potential to be applied in the imaging and tracking of live cells in vitro and of animals in vivo. Notably, QDs may provide the foundation for a novel, non-invasive imaging strategy for the early diagnosis of tumors.

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“…Xue et al, for instance, conjugated Tat peptide to thiol capped CdTe QDs (2-4 nm in diameter) and compared their effectiveness in intracellular delivery to unmodified CdTe QDs in human hepatocellular carcinoma (QGY) cells and human breast cancer (MCF7) cells by means of confocal laser scanning microscopy. The authors were able to demonstrate that the conjugation of Tat enhanced intracellular delivery in both cell lines [85]. The same holds true for the PEG-encapsulated CdSe-ZnS QDs, which were functionalized with covalently bound Tat in order to achieve efficient uptake into mesenchymal stem cells [86].…”

Section: Delivery Of Nanoparticles Using Cpps Quantum Dotsmentioning

confidence: 99%

Cell-penetrating peptides for nanomedicine – how to choose the right peptide

2015

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More than two decades ago, a group of peptides, now known as cell-penetrating peptides, sparked the hope that the ultimate carrier molecules have been found. The high expectations for these peptides, which are reflected in their bold name, led to many disappointments due to the controversial results their utilization entailed and nowadays even their effectiveness has been called into question. In this review, we discuss the uptake mechanism and application of cell penetrating peptides as mediators for organelle specific delivery of nanocarriers, pointing out the possibilities as well as strategies of their successful utilization. Additionally, we provide an overview of the conjugation techniques usually employed for the attachment of cell penetrating peptides to quantum dots, as well as silver and gold nanoparticles, and we address the various aspects that need to be considered for the successful implementation of cell penetrating peptides for organelle-specific delivery of nanoparticles into cells.Keywords: cell penetrating peptide; drug delivery; gold nanoparticle; nanomedicine; polymeric nanoparticle; quantum dot. Highlights-We discuss the mechanisms of cell penetrating peptide's uptake and briefly introduce the different types of peptides in order to present a more complete picture of the intracellular fate of the peptide-cargo conjugates. -We highlight the recent applications of cell penetrating peptides in the targeted delivery of quantum dots as well as gold, silver and polymeric nanoparticles into mammalian cells in the context of organelle specificity. -We give a short overview of the conjugation techniques usually utilized for the attachment of cell penetrating peptides to quantum dots as well as silver and gold nanoparticles.

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Enhancement of Intracellular Delivery of CdTe Quantum Dots (QDs) to Living Cells by Tat Conjugation

Cited by 59 publications

References 27 publications

Intracellular delivery of quantum dots mediated by a histidine- and arginine-rich HR9 cell-penetrating peptide through the direct membrane translocation mechanism

Intracellular delivery of quantum dots mediated by a histidine- and arginine-rich HR9 cell-penetrating peptide through the direct membrane translocation mechanism

Effect of quantum dots on the biological behavior of the EJ human bladder urothelial carcinoma cell line

Cell-penetrating peptides for nanomedicine – how to choose the right peptide

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