Enhancement of Laccase Production from Pleurotus ostreatus PVCRSP-7 by altering the Nutritional Conditions using Response Surface Methodology

Abstract

Submerged culture conditions for laccase production by Pleurotus ostreatus were optimized by response surface methodology (RSM). A total of six factors, carbon (glucose), nitrogen sources (urea and peptone), 2,5-xylidine (inducer), wheat bran (lignocellulosic material), and medium pH, were optimized. A total of 50 experiments were conducted, and the obtained data were modeled using a second-order polynomial. The optimized conditions show significant improvement in laccase expression, by approximately 3.5-fold (12,124 U/L).

Lignin‐modifying enzymes: a green and environmental responsive technology for organic compound degradation

“…It was reported that in SMF, the Lac produced by Pleurotus sajor‐caju , using pulp wash residue as a substrate and optimized by a statistical tool through the response surface methodology, reached an activity of up to 307 379.91 IU L −1 66 . In another study, Chiranjeevi et al 67 . reported that the Lac produced by Pleurotus ostreatus PVCRSP‐7 using wheat bran as a lignocellulosic inducer was able to increase its activity by 3.5 times (approximately 12 124 IU L −1 ) when compared with production without adding the substrate.…”

Isolation of the Antifungal Compound Alliodorin from the Heartwood of Cordia elaeagnoides A. DC. and the In Silico Analysis of the Laccase

“…Its sturdy timber is coveted for crafting mosaic-styled parquet floors, ornamental veneers, high-end furniture, cabinetry, of T1 is reduced to water. Through this enzyme process, the fungus obtains nutrients for its development [29][30][31][32][33]. Therefore, some studies suggest that laccase inhibitors are small molecules capable of binding to the Cu ions of the TCN, thereby interrupting the internal oxidation process such as sodium azide, some small halides, heavy metals, and ethylenediaminetetraacetic acid (EDTA) [34,35].…”