Enhancement of lipopolysaccharide-stimulated cyclooxygenase-2 mRNA expression and prostaglandin E2 production in gingival fibroblasts from individuals with Down syndrome

Otsuka, Yoko; Itô, Masayuki; Yamaguchi, Masaru; Saito, Shigeno; Uesu, Kagumi; Kasai, Kazuhiko; Abiko, Yoshimitsu; Mega, Junichi

doi:10.1016/s0047-6374(01)00413-4

Cited by 21 publications

(17 citation statements)

References 42 publications

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“…Down syndrome individuals are likely to exhibit exaggerated inflammatory responses upon periodontal infection [87][88][89][90][91]. A. actinomycetemcomitans lipopolysaccharide significantly enhanced PGE 2 production through the induction of mRNA expression of COX-2 (PGE 2 -producing enzyme) by gingival fibroblasts from Down syndrome subjects [88]. We also found that P. gingivalis infection significantly induced mRNA expressions of COX-2 as well as IL-6 by gingival fibroblasts in these individuals (Fig.…”

Section: Inflammatory Mediators and Proteolytic Enzymessupporting

confidence: 51%

“…These mediators further activate host cells to mount excessive host inflammatory responses. Down syndrome individuals are likely to exhibit exaggerated inflammatory responses upon periodontal infection [87][88][89][90][91]. A. actinomycetemcomitans lipopolysaccharide significantly enhanced PGE 2 production through the induction of mRNA expression of COX-2 (PGE 2 -producing enzyme) by gingival fibroblasts from Down syndrome subjects [88].…”

Section: Inflammatory Mediators and Proteolytic Enzymesmentioning

confidence: 99%

“…4) [92]. Excessive expressions of MMPs are responsible for periodontal breakdown [93], while collagenase activities comprised mainly of MMP-8, MMP-9, and MMP-2 in saliva and gingival crevicular fluid were reported to be higher in Down syndrome children as compared to the age-matched healthy subjects [88]. Also, the expressions of membrane-type MMP-1 and MMP-2 by gingival fibroblasts of Down syndrome individuals were found to be augmented, as compared with those of healthy controls [89].…”

Section: Inflammatory Mediators and Proteolytic Enzymesmentioning

confidence: 99%

See 2 more Smart Citations

Etiologic factors of early-onset periodontal disease in Down syndrome

Amano

Murakami

Akiyama

et al. 2008

Japanese Dental Science Review

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Section: Inflammatory Mediators and Proteolytic Enzymessupporting

confidence: 51%

Section: Inflammatory Mediators and Proteolytic Enzymesmentioning

confidence: 99%

Section: Inflammatory Mediators and Proteolytic Enzymesmentioning

confidence: 99%

See 1 more Smart Citation

Etiologic factors of early-onset periodontal disease in Down syndrome

Amano

Murakami

Akiyama

et al. 2008

Japanese Dental Science Review

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“…Lipopolysaccharides from periodontal pathogens can penetrate gingival tissues and up-or downregulate pro-inflammatory cytokine production by gingival fibroblasts (Otsuka et al, 2002;Tamai et al, 2002). We sought to determine whether human gingival and dermal fibroblasts can express IL-1b, IL-18, and ICE mRNA and produce and secrete the corresponding proteins.…”

mentioning

confidence: 99%

Gingival and dermal fibroblasts produce interleukin‐1β converting enzyme and interleukin‐1β but not interleukin‐18 even after stimulation with lipopolysaccharide

Tardif

Ross

Rouabhia

2003

Journal Cellular Physiology

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Epithelial cells play a critical role in periodontal disease through the secretion of pro-inflammatory cytokines such as interleukin-1 beta (IL-1 beta) and interleukin-18 (IL-18). However, the role played by fibroblasts is still unclear. The rationale of this study was to throw light on the role of gingival fibroblasts in periodontal disease. We thus investigated the expression of IL-1 beta, IL-18, and ICE mRNA and the secretion of the corresponding proteins by human normal gingival fibroblasts before and after stimulation with lipopolysaccharide (LPS) from Porphyromonas gingivalis and Escherichia coli. IL-1 beta, IL-18, and ICE mRNA expression was evaluated by RT-PCR. Proteins were analyzed by Western blot and ELISA. We demonstrated that gingival fibroblasts expressed ICE mRNA. Basal expression of ICE was modulated following cell stimulation with lipopolysaccharide (5 mug/ml). However, gingival fibroblasts expressed low levels of IL-1 beta mRNA. The expression was potentiated by LPS. The expression of IL-1 beta mRNA was followed by the secretion of IL-1 beta but not IL-18 protein. Our study suggests that fibroblasts may be involved in the defense against infections via an IL-1 beta-mediated but not an IL-18-mediated mechanism.

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“…It is well known that the severity of periodontal diseases is affected by the host s age 2) . In addition, recent studies have shown that the production of inflammatory mediators such as PGE2, IL-1β, IL-6, cyclooxygenase-2 and the plasminogen activator are higher in the gingival fibroblasts from the older animals or in vitro senescent fibroblasts compared with younger counterparts [3][4][5][6][7] .…”

Section: Introductionmentioning

confidence: 99%