Enhancement of lysozyme crystallization under ultrasound field

Ultrasonic protein crystallization: Promoting nucleation in microdroplets through pulsed sonication

“…For the detection method used in this study, the measured induction time (Table 2) is higher than 1 h (imposed nucleation time). Additionally, Mao and co-workers (Mao et al, 2020) reported that ultrasound actuation during the crystal growth stage results in smaller crystals, which is possibly due to the simultaneous occurrence of secondary nucleation (and/or breakage) and crystal growth based on the combination between ultrasonic radiation and stirring. The induction time drastically decreases when applying ultrasound radiation, where the effect is already clear for the lowest forward power (Table 2).…”

“…Magnetic fields do not show a clear effect on nucleation, only on the crystal orientation (Astier et al, 1998;Revalor et al, 2010;Yan et al, 2016) and quality (Yin, 2015), while light irradiation induces nucleation by forming radicals (Okutsu, 2007;Revalor et al, 2010;Veesler et al, 2006). Finally, ultrasound irradiation increases the nucleation rate (Nanev and Penkova, 2001) and, consequently, reduces the induction time and the metastable zone width (Crespo et al, 2010;Kakinouchi et al, 2006;Kitayama et al, 2013;Mao et al, 2020;Revalor et al, 2010). Moreover, the production of uniform tiny crystals might result in these dispersed crystals acting as seeds for industrial macromolecular crystallization processes (Mao et al, 2020).…”

“…Finally, ultrasound irradiation increases the nucleation rate (Nanev and Penkova, 2001) and, consequently, reduces the induction time and the metastable zone width (Crespo et al, 2010;Kakinouchi et al, 2006;Kitayama et al, 2013;Mao et al, 2020;Revalor et al, 2010). Moreover, the production of uniform tiny crystals might result in these dispersed crystals acting as seeds for industrial macromolecular crystallization processes (Mao et al, 2020). Zhang and co-workers (2015) (Zhang et al, 2015), Ruecroft and coworkers (2015) (Ruecroft et al, 2005) and Ratsimba and co-workers (1999) (Ratsimba et al, 1999) reviewed the new perspectives and developments in terms of the industrial application of sonocrystallization.…”

“…Furthermore, in the unsonicated condition the formation of larger crystals occurs due to the suppression of new nucleation events (Kitayama et al, 2013). More recently, Mao and co-workers (Mao et al, 2020) conducted an extended study of the influence of pulsed sonication during nucleation and crystal growth on the induction time, metastable zone width, crystal size, morphology, and process yield. The batch (under magnetic stirring) experiments performed in a volume of 30 ml showed that ultrasound irradiation leads to shorter induction times.…”

Particle Size and Crystal Habit Modification of Active Pharmaceutical Ingredient Using Cooling Sonocrystallization: A Case Study of Probenecid

“…[ 16‐18 ] With the emerging of sonocrystallization in practical use, several case studies have been reported in the literature in recent years for recrystallization of biological compounds such as aspirin, l ‐arginine hydrochloride, ferulic acid, cinnamic acid, oxalic acid, l ‐phenylalanine, salicylamide, isoniazid derivative, telmisartan, lactose, lysozyme, and l ‐glutamic acid. [ 19‐30 ]…”