Enhancement of muscle regeneration in the rat gastrocnemius muscle by low energy laser irradiation

Weiss, Nathan; Oron, Uri

doi:10.1007/bf00185463

Cited by 92 publications

(71 citation statements)

References 18 publications

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“…In the present study, necrotic fibres were removed faster in groups II (LELI) and IV (coenzyme Q10 and vitamin E), compared to other evaluated groups, which was probably associated with increased activity of phagocytic cells. This confirmed the results obtained by other authors, demonstrating the positive impact of the LELI on muscle regeneration by increasing the metabolic activity of macrophages (Weiss and Oron 1992, Bibikova and Oron 1994, Podbielski et al 2006). On the other hand, LELI has an ability to modulate the inflammatory process and to optimize tissue repair by inhibiting some inflammatory mediators: prostaglandin, arachidonic acid and cyclooxygenase-2 (Rennó et al 2014).…”

Section: Discussionsupporting

confidence: 91%

Effect of low-energy laser irradiation and antioxidant supplementation on cell apoptosis during skeletal muscle post-injury regeneration in pigs

Otrocka-Domagała

Mikołajczyk

Paździor-Czapula

et al. 2015

Polish Journal of Veterinary Sciences

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The aim of this study was to evaluate the effect of low-energy laser irradiation, coenzyme Q10 and vitamin E supplementation on the apoptosis of macrophages and muscle precursor cells during skeletal muscle regeneration after bupivacaine-induced injury. The experiment was conducted on 75 gilts, divided into 5 experimental groups: I -control, II -low-energy laser irradiation, III -coenzyme Q10, IV -coenzyme Q10 and vitamin E, V -vitamin E. Muscle necrosis was induced by injection of 0.5% bupivacaine hydrochloride. The animals were euthanized on subsequent days after injury. Samples were formalin fixed and processed routinely for histopathology. Apoptosis was detected using the TUNEL method. The obtained results indicate that low-energy laser irradiation has a beneficial effect on macrophages and muscle precursor cell activity during muscle post-injury regeneration and protects these cells against apoptosis. Vitamin E has a slightly lower protective effect, limited mainly to the macrophages. Coenzyme Q10 co-supplemented with vitamin E increases the activity of macrophages and muscle precursor cells, myotube and young muscle formation. Importantly, muscle precursor cells seem to be more sensitive to apoptosis than macrophages in the environment of regenerating damaged muscle.

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Section: Discussionsupporting

confidence: 91%

Effect of low-energy laser irradiation and antioxidant supplementation on cell apoptosis during skeletal muscle post-injury regeneration in pigs

Otrocka-Domagała

Mikołajczyk

Paździor-Czapula

et al. 2015

Polish Journal of Veterinary Sciences

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“…Studies have shown that muscle regeneration, in vivo as well as in vitro, becomes more effective when treated with low intensity laser 6,7 . It has been demonstrated 8,9 that lasers operating at different wavelengths activate the macrophages to release factors that stimulate fibroblast proliferation.…”

Section: Introductionmentioning

confidence: 99%

Effects of GaAs laser and stretching on muscle contusion in rats

Corrêa¹,

Okita

Martins

et al. 2016

Fisioter. Pesqui.

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UFPR -Curitiba (PR), Brazil. 3ABSTRACT | Laser and stretching are used to treat skeletal muscle injuries. This study aimed to evaluate the effects of GaAs laser and stretching in the morphology of the tibialis anterior (TA) muscle after contusion. Thirty-six male rats (349±23g) were divided into six groups (n=6): control group (CG); lesion group (LG); lesion and laser group (LLG); lesion and stretching group (LSG); lesion, laser and stretching group (LLSG); and stretching group (SG). TA was wounded by a contusion apparatus. We used GaAs laser 4.5 J/cm 2 dose for 32 s each, beginning 48 h after lesion, for 7 days, once a day. Manual passive stretching was applied by 10 repetitions for 1 minute, initiating on the 8 th day, once a day, 3 times a week, during 3 weeks. After 4 weeks, rats were euthanized and we analyzed: muscle weight and length, cross sectional Se utilizó el tratamiento con láser GaAs con dosis de 4,5 J/cm 2 durante 32 s, iniciado 48 h después de la lesión, durante 7 días.El estiramiento pasivo manual consistió de 10 repeticiones de 1 minuto de duración, iniciado en el 8º día, 3 veces por semana, durante 3 semanas. Después de 4 semanas, los ratones sufrieron eutanasia para la retirada del TA para análisis de: peso y longitud musculares, área de sección trasversa de las fibras musculares (ASTFM), número de sarcómeros en serie (NSS), longitud de los sarcómeros y porcentaje de tejido conjuntivo. La comparación entre los grupos ocurrió mediante la Anova y post hoc Tukey, con nivel de significancia de ≤0,05. El número de sarcómeros en serie del GLLS fue mayor que el GLS. La longitud de los sarcómeros en el GLA fue superior al GLL, GLLA y GA. En el GA hubo aumento del NSS en comparación con el GC, mientras el porcentaje del tejido conjuntivo del GA disminuyó en comparación al del GLLA. Así, la sarcomerogénesis de los músculos lesionados fue aumentada por el uso del láser, estiramiento y por su asociación. El estiramiento fue suficiente para aumentar el NSS en músculos intactos. Palabras clave |

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“…Photobiomodulation effects of Low-level light irradiation (LLLI) on regeneration have been reported in skin [1] [2], nerve [3], skeletal muscle tissues [4] and bone [5]. LLLI can be used as a efficiently tool for the preconditioning of bone marrow mesenchymal stem cells (MSCs), which derived from bone marrow and received wildly attentions in regeneration medicine.…”

Section: Introductionmentioning

confidence: 99%

The Effect of 635 nm Red Laser Irradiation on Proliferation of Bone Marrow Stem Cells

Peng¹

2016

OPJ

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Photobiomodulation effects of Low-level light irradiation (LLLI) on regeneration have been reported in skin, nerve, and skeletal muscle tissues and bone. Bone Mesenchymal stem cells (BMSCs)are derived from bone marrow, which exhibited a fibroblast-like appearance, and could differentiate in vitro into different lineages. However, there is a reciprocal relationship between growth and osteogenic differentiation in MSCs. Therefore, it's important to investigate the effect of LLLI on BMSCs. The aim of our study was to investigate the proliferation effect of 635 nm red laser light on bone marrow MSCs with or without osteogenic supplements. Bone marrow was collected from the 4-week-old Sprague-Dawley rats femur and tibiae. MSCs with and without osteogenic supplements both were divided into three groups. A continuous 635 nm wavelength red light diode laser (a power output of 960 mW) was used in the study. The size of light spot was 35mm in diameter. Irradiation was performed every other day since the half of medium was changed to osteogenic differentiation media (ODM). The first irradiation day was set as 0 day. The duration of each irradiation for red light was calculated at 10 seconds for 1 J/cm 2 , 20 seconds for 2 J/cm 2 . Two of these groups were used as controls: MSCs incubated in DMEM without irradiation (control 1), MSCs incubated in ODM without irradiation (control 2). Cellular proliferation was evaluated by using WST-8. Cell viability was assessed with WST-8 kit at 2, 4, 6 and 8 days, respectively. At 4, 6 and 8 days, groups cultured with DMEM showed significantly higher viabilities than that in groups with ODM. In groups with DMEM, red light at all doses significantly stimulated cell viability as compared with the control 1. Groups irradiated at 1 and 2 J/cm 2 had more effective proliferation on 4 (P < 0.01) and 6 days (P < 0.05), when compared with the control 1. In groups with ODM, control 2 and the irradiated groups showed similar proliferation speeds. In conclusion, we can find that red light can promote proliferation of MSCs cultured in normal media, and suppress proliferation of MSCs cultured in ODM.

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Enhancement of muscle regeneration in the rat gastrocnemius muscle by low energy laser irradiation

Cited by 92 publications

References 18 publications

Effect of low-energy laser irradiation and antioxidant supplementation on cell apoptosis during skeletal muscle post-injury regeneration in pigs

Effect of low-energy laser irradiation and antioxidant supplementation on cell apoptosis during skeletal muscle post-injury regeneration in pigs

Effects of GaAs laser and stretching on muscle contusion in rats

The Effect of 635 nm Red Laser Irradiation on Proliferation of Bone Marrow Stem Cells

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