Enhancement of Myofibrillar Proteolysis Following Infusion of Amino Acid Mixture Correlates Positively with Elevation of Core Body Temperature in Rats

Yamaoka, Ippei; Mikura, Mayumi; Nishimura, Masuhiro; Doi, Masako; Kawano, Yoshio; Nakayama, Mitsuo

doi:10.3177/jnsv.54.467

Cited by 13 publications

(11 citation statements)

References 41 publications

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“…The supernatants were freeze-dried and dissolved in 100 μL of 50 mmol/L SDS/acetonitrile/phosphoric acid (610:390:3). N τ -methylhistidine levels were measured by high-performance liquid chromatography as previously described (Yamaoka et al, 2008) …”

Section: Animals and Samplingmentioning

confidence: 99%

The IGF-1/Akt/S6 Signaling Pathway is Age-Dependently Downregulated in the Chicken Breast Muscle

et al. 2016

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The skeletal muscle mass is known to be controlled by the balance between protein synthesis and degradation. The fractional rate of protein synthesis has been reported to decrease age-dependently from 1 to 4 weeks of age in the chicken breast muscle (pectoralis major muscle). On the other hand, age-dependent change of the fractional protein degradation rate was reported to be less in the skeletal muscle of chickens. These findings suggest that protein synthesis is age-dependently downregulated in chicken muscle. We herein investigated the age-dependent changes in protein synthesis or proteolysis-related factors in the breast muscle of 7, 14, 28, and 49-day old broiler chickens. IGF-1 mRNA level, phosphorylation rate of Akt, and phospho-S6 content were coordinately decreased in an agedependent manner, suggesting that IGF-1-stimulated protein synthesis is downregulated with age in chicken breast muscle. In contrast, atrogin-1, one of the proteolysis-related factors, gradually increased with age at mRNA levels. However, plasma N τ -methylhistidine concentration, an indicator of skeletal muscle proteolysis, did not coordinately change with atrogin-1 mRNA levels. Taken together, our results suggest that the IGF-1/Akt/S6 signaling pathway is age-dependently downregulated in the chicken breast muscle.

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Section: Animals and Samplingmentioning

confidence: 99%

The IGF-1/Akt/S6 Signaling Pathway is Age-Dependently Downregulated in the Chicken Breast Muscle

et al. 2016

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“…However, a previous study shows that there is no statistically significant correlation between the inhibitory effect of amino acid infusion during general anesthesia on hypothermia and muscle weight (21). It has also been shown in rats that the myofibrillar protein degradation product, plasma 3-methylhistidine, increases with amino acid infusion and thus the values of plasma 3-methylhistitine correlate positively with central body temperature (22). These reports suggest that enhanced protein turnover in muscle by amino acid infusion is more important than the weight of skeletal muscle.…”

Section: Discussionmentioning

confidence: 55%

Effects of Intravenous Amino Acids on Anesthesia-Induced Hypothermia in Ovariectomized Rats

Ando

Kanazawa

Tsuda

et al. 2012

J Nutr Sci Vitaminol

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Summary A decrease in core temperature during general anesthesia is attenuated by infusion of an intravenous amino acid mixture. The purpose of the present study was to investigate the influence of physical and endocrine changes caused by ovariectomy on the inhibitory effect of amino acid infusion on anesthesia-induced hypothermia. Sprague-Dawley female rats were divided into a sham-operated (Sham) group and an ovariectomized (OVX) group. Saline solution or an amino acid mixture solution was infused for 180 min during sevoflurane anesthesia, and the rectal temperature was measured (4 groups). Intraperitoneal white adipose tissue mass, bilateral gastrocnemius weight and plasma insulin levels were measured. In the Sham rats, no inhibitory effect of the amino acid mixture on anesthesia-induced hypothermia was found, while in the OVX rats, hypothermia was significantly decreased. The intraperitoneal fat weight/body weight ratio was significantly higher in the OVX rats than in the Sham rats, but the gastrocnemius weight/body weight ratio was not significantly different. After amino acid infusion, the plasma insulin level was significantly higher in the OVX rats than in the Sham rats. In conclusion, our findings suggest that, in rats, ovarian function or female hormone affects protein turnover mediated by increase in insulin secretion and, thus, decreases the inhibitory effect of an infusion of amino acid mixture on anesthesia-induced hypothermia. Key Words hypothermia, amino acids, general anesthesia, ovariectomy Redistribution hypothermia occurs in the body after induction of general anesthesia. Core body temperature gradually declines as heat is lost from the body surface, and heat production slows because of decreased metabolism. Selldén et al. reported that an amino acid infusion during anesthesia caused nutrient-induced thermogenesis (NIT), preventing the temperature reduction of redistribution hypothermia ( 1 ). Fujiwara et al. noted a similar effect after amino acid infusion but not after an infusion of glucose and fat emulsion ( 2 ). It is known that the metabolism of amino acids requires a large expenditure of energy ( 3 ), and protein synthesis induced by amino acids has been shown to correlate positively with energy expenditure ( 4 ). When rats were given the protein synthesis inhibitor puromycin, NIT did not occur after amino acid infusion ( 5 ). It has also been suggested that 75% of the increased oxygen uptake by amino acid infusion is produced in tissues other than the internal organs ( 6 ). Amino acid infusion during general anesthesia was found to stimulate protein synthesis in skeletal muscle via enhanced insulin secretion and to prevent hypothermia by heat accumulation through NIT ( 7 ). We have reported that skeletal muscle and plasma insulin levels play a major role in changes in body temperature during general anesthesia and the effect of amino acids on body temperature in a rat model of muscle atrophy ( 8 ).On the other hand, sex hormone is known to be involved in thermoregulation. In addition,...

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“…Intriguingly, not only phosphorylation of these signaling transduction components and translation initiation factors but also amino acid-stimulated increase in oxygen consumption and core temperature is clearly cancelled by inhibiting the amino acid-induced elevation of plasma insulin by a pancreatic hormone inhibitor, somatostatin [31]. In terms of amino acids oxidation, intravenous infusion of amino acid boosts degradation of myofibrillarprotein [32].…”

Section: Mechanism For Preventing Hypothermiamentioning

confidence: 99%

“…In that case both degradation and anabolism should be approximately equal. Interestingly, core temperature of anesthetized and conscious rats well correlates with plasma 3-methylhistidine, a marker of myofibrillar protein breakdown [32]. It is described that glucose infusion clearly inhibits degradation of myofibrillar protein through down-regulation of ubiquitin proteasome genes expression in rats [33].…”

Section: Mechanism For Preventing Hypothermiamentioning

confidence: 99%

Amino Acids during Perioperative Period

Yokoyama¹,

Yamaoka²,

Hitosugi³

et al. 2017

OJAnes

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During anesthesia, thermoregulation is impaired and hypothermia will frequently occur in most patients. Hypothermia affects immunologic activity, bleeding tendency and the recovery from anesthesia. Therefore, it may prolong hospital stay, and increase morbidity, e.g. surgical site infections, cardiac events and multiple organ dysfunctions in trauma. External warming is often used to prevent hypothermia. However, infusion of amino acids is also valuable to prevent hypothermia due to their enhanced thermogenic action under anesthesia. During surgery, amino acids administration would maintain the body homeostasis, and counteract the disadvantageous fasting metabolism. Postoperatively, amino acids may be advantageous for the healing of the surgical wound. Thus, appropriate nutritional management, including glucose, during the perioperative period would prevent catabolism, frequently occurring after surgery. Protocols like ERAS (Enhanced Recovery After Surgery) are proposed for quick recovery after surgery. ERAS protocol recommends preoperative carbohydrate and early enteral nutrition, but does not include infusion of amino acids during the perioperative period. During prolonged surgery, patients clearly need good nutritional support. In this article, we intend to describe the problems of hypothermia briefly, and explain the mechanism of amino acids in hypothermia prevention. In addition, we address some evidences of nutritional management during the perioperative period.

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Enhancement of Myofibrillar Proteolysis Following Infusion of Amino Acid Mixture Correlates Positively with Elevation of Core Body Temperature in Rats

Cited by 13 publications

References 41 publications

The IGF-1/Akt/S6 Signaling Pathway is Age-Dependently Downregulated in the Chicken Breast Muscle

The IGF-1/Akt/S6 Signaling Pathway is Age-Dependently Downregulated in the Chicken Breast Muscle

Effects of Intravenous Amino Acids on Anesthesia-Induced Hypothermia in Ovariectomized Rats

Amino Acids during Perioperative Period

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