Enhancement of neural cell lines proliferation using nano-structured chitosan/poly(vinyl alcohol) scaffolds conjugated with nerve growth factor

Mottaghitalab, Fatemeh; Farokhi, Mehdi; Mottaghitalab, Vahid; Ziabari, Mohammad; Divsalar, Adeleh; Shokrgozar, Mohammad Ali

doi:10.1016/j.carbpol.2011.04.066

Cited by 69 publications

(39 citation statements)

References 36 publications

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“…So this technique would be more applicable for gene or growth-factor delivery, where a slow and prolonged release of the therapeutic agent is required. 57,[59][60][61][62][63] For biomolecules like DNA, growth factors (GFs), or enzymes that lose bioactivity and degrade within a few days, conjugating the biomolecules to the fiber surfaces and slowly releasing them into the nearby tissue would significantly preserve their functionality.…”

Section: Electrospinningmentioning

confidence: 99%

“…110 Although the controlled release of GFs is achievable, the instability of GFs hinders the successful development of GF-loaded tissue-engineering scaffolds. Various techniques, such as blending, 91,111 specific or nonspecific surface modifications, 57,[60][61][62][63]105 coaxial electrospinning, 56 emulsion electrospinning, 79,88 and combination of electrospinning with other conventional techniques, 112,113 were applied for GF incorporation into nanofibrous scaffolds, yielding varied level of success. Burst release of EGF was obtained from silk nanofibers blended with EGF, due to the hydrophobic nature of EGF.…”

Section: 108mentioning

confidence: 99%

“…Mimicking the native tissues, attempts have been made to immobilize GF onto scaffolds using polysaccharides and/or heparin. 57,60,63 Mottaghitalab et al assessed nerve GF (NGF) surface-conjugated CS/PVA scaffolds with respect to adhesion and proliferation of SKNMC (human neuroblastoma) and U373 (human glioblastomaastrocytoma) cell lines. However, using this technique, an initial release of localized NGF on the surface followed by diffusion-based release of NGF was observed.…”

Section: 108mentioning

confidence: 99%

“…However, using this technique, an initial release of localized NGF on the surface followed by diffusion-based release of NGF was observed. 60 To solve the problem of GF burst release, electrospun CS fibers were surface-immobilized with heparin-containing polyelectrolyte complex nanoparticles (PCNs) presenting basic fibroblast GF (FGF-2), which were adsorbed to the nanofibers' surface. As a result, 30 days' zero-order release of FGF-2/PCN was achieved taking advantage of the preservative properties of heparin against proteolytic and chemical inactivation of GFs.…”

Section: 108mentioning

confidence: 99%

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Advances in drug delivery via electrospun and electrosprayed nanomaterials

Zamani¹,

Prabhakaran²,

Ramakrishna³

2013

IJN

204

104

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Electrohydrodynamic (EHD) techniques refer to procedures that utilize electrostatic forces to fabricate fibers or particles of different shapes with sizes in the nano-range to a few microns through electrically charged fluid jet. Employing different techniques, such as blending, surface modification, and coaxial process, there is a great possibility of incorporating bioactive such molecules as drugs, DNA, and growth factors into the nanostructures fabricated via EHD techniques. By careful selection of materials and processing conditions, desired encapsulation efficiency as well as preserved bioactivity of the therapeutic agents can be achieved. The drug-loaded nanostructures produced can be applied via different routes, such as implantation, injection, and topical or oral administration for a wide range of disease treatment. Taking advantage of the recent developments in EHD techniques like the coaxial process or multilayered structures, individually controlled delivery of multiple drugs is achievable, which is of great demand in cancer therapy and growth-factor delivery. This review summarizes the most recent techniques and postmodification methods to fabricate electrospun nanofibers and electrosprayed particles for drug-delivery applications.

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Section: Electrospinningmentioning

confidence: 99%

Section: 108mentioning

confidence: 99%

Section: 108mentioning

confidence: 99%

Section: 108mentioning

confidence: 99%

See 2 more Smart Citations

Advances in drug delivery via electrospun and electrosprayed nanomaterials

Zamani¹,

Prabhakaran²,

Ramakrishna³

2013

IJN

204

104

View full text Add to dashboard Cite

show abstract

“…Polysaccharides like chitosan based materials are known for biocompatible, and promote cell adhesion, proliferation, and differentiation has been used extensively for orthopaedic tissue engineering [19]. Chitosan based hybrid nanocomposites have been reported as bone tissue engineering materials [20], cartilage regeneration [21], and liver and nerve tissue engineering [22].…”

Section: Chitosan Nano Towards Tissue Engineering Applicationsmentioning

confidence: 99%

Chitosan Based Nanoparticles towards Biomedical Applications

Laskar¹,

Rauf²

2017

JNMR

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This review outlines the bioapplications of newly developed chitosan based nanoparticles. Over the last decade, much interest has been developed in biopolymer based materials due to their biocompatible, biodegradable, nontoxic and non-allergenic nature. Here, we review the recent scientific advances in chitosan based nanoparticles not only for cancer therapy but also for various biomedical applications. An overview of chitosan, its derivatives and the formation of chitosan nanoparticles is provided along with the inherent and specific therapeutic efficacy. Major progresses in drug delivery approaches and gene delivery as well as tissue engineering applications have been summarized.

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