Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro

Ren, Xiaohua; Tuo, Qiang; Tian, Kun; Huang, Guo; Li, Jinyu; Xu, Taotao; Lv, Xinrong; Wu, Julia; Chen, Zhenghui; Weng, Jie; Wang, Qingwei; Mu, Yandong

doi:10.1016/j.ceramint.2018.08.249

Cited by 30 publications

(23 citation statements)

References 38 publications

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“…Feng et al (22) have also revealed that grooved micropatterns of fibres affect the adhesion and proliferation of human vascular endothelial cells. in our previous study, the grooved structure of the HaS was also presented as a surface pattern that promoted hPMScs (8). compared with the effects of a regular HAS, the HAS-G significantly promoted osteogenic differentiation and the maturation of osteoblasts in DM, which was confirmed by measuring osteogenic proteins in vitro and new bone formation and vasculogenesis in vivo (8).…”

Section: Discussionmentioning

confidence: 67%

“…in our previous study, the grooved structure of the HaS was also presented as a surface pattern that promoted hPMScs (8). compared with the effects of a regular HAS, the HAS-G significantly promoted osteogenic differentiation and the maturation of osteoblasts in DM, which was confirmed by measuring osteogenic proteins in vitro and new bone formation and vasculogenesis in vivo (8). interestingly, the results of the present study suggested that, in nM, cultured BMScs exhibited little osteogenic differentiation on the HaS or the HaS-G, indicating that the HaS-G promoted osteogenic differentiation and that osteoblast maturation under stimulating conditions mainly exerted protective effects.…”

Section: Discussionmentioning

confidence: 82%

“…The mechanisms by which the HaS-G promotes osteogenesis have not been reported previously. it has been reported that HaS-G significantly promoted hPMSc osteogenic differentiation and the maturation of osteoblasts both in vitro and in vivo, but the exact mechanism is not completely understood (8). in the present study, a HaS with a 25-30-µm microgroove was used as the growing surface of BMScs.…”

Section: Discussionmentioning

confidence: 86%

“…This suggested that the surface of HASs may be modified by producing a grooved structure. in our previous study, HaSs with a 25-30-µm groove structure (HaS-G) countered the limitations of regular HaS showing clinical inhibition of osteogenic effects (8). HAS-G significantly promoted human placenta-derived mesenchymal stem cell (hPMSc) osteogenic differentiation and the maturation of osteoblasts in vitro and in vivo compared with HaS (8).…”

Section: Introductionmentioning

confidence: 94%

“…HaS-G and HaS were prepared as previously described (8), and the surface patterns were confirmed by SEM (Fig. 1).…”

Section: Has-g Surface Pattern Promotes Proliferation In Bmscsmentioning

confidence: 99%

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Grooved hydroxyapatite scaffold modulates�mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells

Lü

Ren

et al. 2020

Mol Med Rep

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Hydroxyapatite scaffolds (HaSs) are widely studied as suitable materials for bone replacement scaffolds due to their chemical similarities to organic materials. in our previous study, a novel HaS with a 25-30-µm groove structure (HaS-G) exhibited enhanced osteogenesis of bone mesenchymal stromal cells (BMScs) compared with HaS, potentially by modulating the macrophage-induced immune microenvironment. However, the exact effects of different surface patterns on the physiological processes of attached cells is not known. The present study aimed to determine the effects of HaS-G on the osteogenesis and physiological processes in BMScs. cell counting kit-8 assays and propidium iodide staining followed by flow cytometry were performed, and the results demonstrated that both in normal medium and differentiating medium, HaS-G promoted cell proliferation by decreasing the proportion of G1/G0 cells and decreased reactive oxygen species (roS) accumulation in BMScs compared with HaS. detection markers of osteogenesis revealed that compared with HaS, HaS-G increased runt-related transcription factor 2, osteocalcin and osteopontin protein levels and promoted osteogenesis, which was further confirmed by Alizarin Red S staining. Following Jc-1 staining, it was observed that HaS-G maintained the mitochondrial membrane potential, similar to that achieved by n-acetylcysteine pretreatment. in addition, compared with those of HaS, HaS-G decreased mitochondrial roS levels, which potentially contributed to the promotion of osteogenesis. The results also demonstrated that HaS-G inhibited mitophagy induced by roS accumulation and aTP synthesis compared with HaS. in conclusion, HaS-G decreased roS accumulation and mitophagy and thus promoted osteogenesis of BMScs, indicating that roS modulation of HaS-G may serve a key role in osteogenesis.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 94%

“…HaS-G and HaS were prepared as previously described (8), and the surface patterns were confirmed by SEM (Fig. 1).…”

Section: Has-g Surface Pattern Promotes Proliferation In Bmscsmentioning

confidence: 99%