Enhancement of osteogenic gene expression for the differentiation of human periosteal derived cells

Roberts, Simon C.; Chen, Yantian; Moesen, Maarten; Schrooten, Jan; Luyten, Frank P.

doi:10.1016/j.scr.2011.04.003

Cited by 42 publications

(36 citation statements)

References 31 publications

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“…Also, once the matrix mineralization is completed, extracellular calcium deposition Fig. 4 The effect of 20 and 40 mg of partially demineralized (A and B test groups), fully demineralized (C-G test groups) and mineralized (H and I test groups) freeze-dried bone allografts (per well/2 mL culture medium) on bone mineralization in MG-63 cells at 72 h after treatment (non-clear nodules in G group and the osteogenic medium group are marked with arrows) Cell Tissue Bank (indicator of bone formation) can be visualized (Boufker et al 2010;Roberts et al 2011;Miron and Zhang 2012;Beederman et al 2013). Cytotoxicity evaluation is very important for preliminary analysis of biomaterials (such as allografts) and cytotoxicity must be evaluated prior to assessment of cell proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

In vitro effect of mineralized and demineralized bone allografts on proliferation and differentiation of MG-63 osteoblast-like cells

et al. 2015

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Due to the extensive use of bone allografts in bone reconstruction and periodontal therapy as suitable alternatives to autografts, they are now marketed under different commercial brands. Considering the controversial reports regarding the osteoinductive properties of bone allografts, this study sought to assess the effect of type (mineralized/demineralized), amount and particle size of several allografts on the proliferation and differentiation of MG-63 osteoblast-like cells. MG-63 cells (24-h culture) were exposed to 20 and 40 mg amounts of nine different commercially available freeze-dried bone allografts. After 24 and 72 h of incubation, the effect of water-soluble allograft released materials on cell viability and proliferation was assessed using methyl thiazol tetrazolium (MTT) assay after 24 and 72 h of exposure. Cell differentiation and mineralization was assessed by real-time quantitative reverse transcription PCR and alizarin red staining after 72 h of exposure. The amount and particle size of understudy allografts had significant effects on cell viability after 24 h of exposure (in contrast to 72 h). Higher rate of proliferation was seen in non-differentiated or slow-differentiated groups. The amount and particle size factors had no significant effect on the amount of calcified nodules or the expression of osteogenic marker genes in most groups. Faster and more distinct differentiation and mineralization was noted in mineralized compared to demineralized groups during the 3-day study period. Based on the results, the understudy mineralized (non-demineralized) bone allografts had greater effect on osteogenic differentiation of the MG-63 cells and showed more in vitro osteoinductive activity compared to partially demineralized and fully demineralized types.

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Section: Discussionmentioning

confidence: 99%

In vitro effect of mineralized and demineralized bone allografts on proliferation and differentiation of MG-63 osteoblast-like cells

et al. 2015

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“…In addition, PDC respond to a number of growth factors and cytokines. Indeed, factors such as IL6, epidermal growth factor (EGF), TGFβ1 and BMP2 have been shown to play key roles in PDC osteogenic differentiation [68,69]. Additionally, calciumand phosphate-enriched culture conditions promote certain aspects of osteogenic differentiation in vitro, although this may be as a result of enhanced BMP2 expression [70,71].…”

Section: Isolation and Characterization Of Skeletal Progenitor Cells mentioning

confidence: 99%

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

Roberts

Gastel

Carmeliet

et al. 2015

Bone

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209

186

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The cartilage- and bone-forming properties of the periosteum have long since been recognized. As one of the major sources of skeletal progenitor cells, the periosteum plays a crucial role not only in bone development and growth, but also during bone fracture healing. Aided by the continuous expansion of tools and techniques, we are now starting to acquire more insight into the specific role and regulation of periosteal cells. From a therapeutic point of view, the periosteum has attracted much attention as a cell source for bone tissue engineering purposes. This interest derives not only from the physiological role of the periosteum during bone repair, but is also supported by the unique properties and marked bone-forming potential of expanded periosteum-derived cells. We provide an overview of the current knowledge of periosteal cell biology, focusing on the cellular composition and molecular regulation of this remarkable tissue, as well as the application of periosteum-derived cells in regenerative medicine approaches. This article is part of a Special Issue entitled "Stem Cells and Bone".

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“…Periosteal biopsies (0.5 cm 2 ) were harvested from the medial side of the proximal tibia (mean age 29 ± 12 years; 2 females, 2 males) during total knee replacement surgery or distraction osteogenesis as described previously (33). Briefly, the periosteum was stripped from the tibia with a periosteal lifter.…”

Section: Hpdc Isolation and Culturementioning

confidence: 99%

Serum of patients with active rheumatoid arthritis inhibits differentiation of osteochondrogenic precursor cells

Pathak

Verschueren

Lems

et al. 2016

Connective Tissue Research

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Delayed fracture healing is frequently experienced in patients with systemic inflammation such as during rheumatoid arthritis (RA). The reasons for this are diverse, but could also be caused by inflammatory cytokines and/or growth factors in serum from patients with active disease. We hypothesized that serum from patients with active RA contains circulating inflammatory factors that inhibit differentiation of osteochondrogenic precursors. Serum was obtained from 15 patients with active RA (active RA-sera) and from the same patients in clinical remission 1 year later (remission RA-sera; controls). The effect of active RA-sera on osteochondrogenic differentiation of chondrogenic ATDC5 cells and primary human periosteum-derived progenitor cells (HPDC) was determined in micromass culture. In ATDC5 cells, active RA-sera reduced Ki67 transcription levels by 40% and cartilage matrix accumulation by 14% at day 14, and Alp transcription levels by 16%, and matrix mineralization by 17% at day 21 compared with remission RA-sera. In HPDCs, active RA-sera inhibited metabolic activity by 8%, SOX9 transcription levels by 14%, and cartilage matrix accumulation by 7% at day 7 compared with remission RA-sera. In conclusion, sera from patients with active RA negatively affect differentiation of osteochondrogenic precursors, and as a consequence may contribute to delayed fracture healing in these patients. ARTICLE HISTORY

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Enhancement of osteogenic gene expression for the differentiation of human periosteal derived cells

Cited by 42 publications

References 31 publications

In vitro effect of mineralized and demineralized bone allografts on proliferation and differentiation of MG-63 osteoblast-like cells

In vitro effect of mineralized and demineralized bone allografts on proliferation and differentiation of MG-63 osteoblast-like cells

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

Serum of patients with active rheumatoid arthritis inhibits differentiation of osteochondrogenic precursor cells

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