Enhancement of Oxygen Transfer Rate Using Microencapsulated Silicone Oils as Oxygen Carriers

Leung, Ricky; Poncelet, Denis; Neufeld, Ronald J.

doi:10.1002/(sici)1097-4660(199701)68:1<37::aid-jctb601>3.0.co;2-y

Cited by 40 publications

(7 citation statements)

References 7 publications

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“…Besides improving the oxygen saturation concentration through increased system pressure (Bolivar et al, 2019; Woodley, 2019) or through additives such as silicone oil (Leung, Poncelet, & Neufeld, 1997; Zhai et al, 2020), the system‐specific aerator characterization in view of the oxygen mass transfer rate is the key to increase reaction rates. In respect to the oxygen solubility of 0.213 mM (35°C) and the above named K m ,O2 of 0.18 mM of GOx from A. niger (Nakamura et al, 1976), the gluconic acid synthesis is challenged by the low solubility of oxygen in water (Woodley, 2019).…”

Section: Resultsmentioning

confidence: 99%

Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity

Thomas

Ohde

Matthes

et al. 2020

Biotech & Bioengineering

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The sufficient provision of oxygen is mandatory for enzymatic oxidations in aqueous solution, however, in process optimization this still is a bottleneck that cannot be overcome with the established methods of macrobubble aeration. Providing higher mass transfer performance through microbubble aerators, inefficient aeration can be overcome or improved. Investigating the mass transport performance in a model protein solution, the microbubble aeration results in higher k L a values related to the applied airstream in comparison with macrobubble aeration. Comparing the aerators at identical k L a of 160 and 60 1/h, the microbubble aeration is resulting in 25 and 44 times enhanced gas utility compared with aeration with macrobubbles. To prove the feasibility of microbubbles in biocatalysis, the productivity of a glucose oxidase catalyzed biotransformation is compared with macrobubble aeration as well as the gas-saving potential. In contrast to the expectation that the same productivities are achieved at identically applied k L a, microbubble aeration increased the gluconic acid productivity by 32% and resulted in 41.6 times higher oxygen utilization. The observed advantages of microbubble aeration are based on the large volume-specific interfacial area combined with a prolonged residence time, which results in a high mass transfer performance, less enzyme deactivation by foam formation, and reduced gas consumption. This makes microbubble aerators favorable for application in biocatalysis.

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Section: Resultsmentioning

confidence: 99%

Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity

Thomas

Ohde

Matthes

et al. 2020

Biotech & Bioengineering

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“…O 2 solubility in silicone oil is ≈6 mmol dm −3 , 20-fold higher than that of oxygen in water. [106] In addition, silicone oil is nonvolatile and stable, and will benefit for long-term battery operation in ambient air. [101] The first example of using OSM in Li-air batteries in ambient air (20-30% relative humidity (RH)) was reported by Zhang et al [101] The membrane allows O 2 to permeate through while blocking H 2 O.…”

Section: Oxygen-selective Membranementioning

confidence: 99%

Critical Advances in Ambient Air Operation of Nonaqueous Rechargeable Li–Air Batteries

Liu

Guo

et al. 2019

Small

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Over the past few years, great attention has been given to nonaqueous lithium-air batteries owing to their ultrahigh theoretical energy density when compared with other energy storage systems. Most of the research interest, however, is dedicated to batteries operating in pure or dry oxygen atmospheres, while Li-air batteries that operate in ambient air still face big challenges. The biggest challenges are H2O and CO2 that exist in ambient air, which can not only form byproducts with discharge products (Li2O2), but also react with the electrolyte and the Li anode. To this end, recent progress in understanding the chemical and electrochemical reactions of Li-air batteries in ambient air is critical for the development and application of true Li-air batteries. Oxygen-selective membranes, multifunctional catalysts, and electrolyte alternatives for ambient air operational Li-air batteries are presented and discussed comprehensively. In addition, sepa-rator modification and Li anode protection are covered. Furthermore, the challenges and directions for the future development of Li-air batteries are presented

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“…To increase oxygen supply to encapsulation systems and cells in a bioreactor including artificial liver devices, oxygen vectors such as perfluorocarbons (PFCs) (19), modified hemoglobin from bRBC (bovine red blood cell) (20), and silicone oil (21) have been utilized. Among these candidates, PFCs have received significant attention in that they offer biocompatibility, bioinertness, and high capacity to dissolve gases (20-fold higher than aqueous medium for oxygen) (22).…”

Section: Introductionmentioning

confidence: 99%

Hydrogel‐Perfluorocarbon Composite Scaffold Promotes Oxygen Transport to Immobilized Cells

Chin

Khattak

Bhatia

et al. 2008

Biotechnology Progress

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Cell encapsulation provides cells a three-dimensional structure to mimic physiological conditions and improve cell signaling, proliferation, and tissue organization as compared to monolayer culture. Encapsulation devices often encounter poor mass transport, especially for oxygen, where critical dissolved levels must be met to ensure both cell survival and functionality. To enhance oxygen transport, we utilized perfluorocarbon (PFC) oxygen vectors, specifically perfluorooctyl bromide (PFOB) immobilized in an alginate matrix. Metabolic activity of HepG2 liver cells encapsulated in 1% alginate/10% PFOB composite system was 47-104% higher than alginate systems lacking PFOB. A cubic model was developed to understand the oxygen transport mechanism in the alginate/PFOB composite system. The theoretical flux enhancement in alginate systems containing 10% PFOB was 18% higher than in alginate-only systems. Oxygen uptake rates (OURs) of HepG2 cells were enhanced with 10% PFOB addition under both 20% and 5% O 2 boundary conditions, by 8% and 15%, respectively. Model predictions were qualitatively and quantitatively verified with direct experimental OUR measurements using both a perfusion reactor and oxygen sensing plate, demonstrating a greater OUR enhancement under physiological O 2 boundary conditions (i.e., 5% O 2 ). Inclusion of PFCs in an encapsulation matrix is a useful strategy for overcoming oxygen limitations and ensuring cell viability and functionality both for large devices (>1 mm) and over extended time periods. Although our results specifically indicate positive enhancements in metabolic activity using the model HepG2 liver system encapsulated in alginate, PFCs could be useful for improving/stabilizing oxygen supply in a wide range of cell types and hydrogels.

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Enhancement of Oxygen Transfer Rate Using Microencapsulated Silicone Oils as Oxygen Carriers

Cited by 40 publications

References 7 publications

Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity

Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity

Critical Advances in Ambient Air Operation of Nonaqueous Rechargeable Li–Air Batteries

Hydrogel‐Perfluorocarbon Composite Scaffold Promotes Oxygen Transport to Immobilized Cells

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