Enhancement of photo-bactericidal effect of tetrasulfonated hydroxyaluminum phthalocyanine on Pseudomonas aeruginosa

Abstract

At the present time, photodynamic inactivation (PDI) is receiving considerable interest for its potential as an antimicrobial therapy. The results of our study indicate that enhancement of the phototoxic effect on Pseudomonas aeruginosa can be achieved by combination of tetrasulfonated hydroxyaluminum phthalocyanine (AlPcS) and bimetallic gold/silver nanoparticles (Au/Ag-NPs) synthesized by the cell-free filtrate of Aureobasidium pullulans. The bimetallic nanoparticles were characterized by a number of techniques including UV-vis, XPS, TEM, and SEM-EDS to be 14 ± 3 nm spherical particles coated with proteins. The effect of diode lasers with the peak-power wavelength ʎ = 650 nm (output power of 10 and 40 mW; radiation intensity of 26 and 105 mW/cm) in combination with the AlPcS and the bimetallic nanoparticles mixture on the viability of P. aeruginosa rods was shown. Particularly high efficiency of killing bacterial cells was obtained for the light intensity of 105 mW/cm, after 20, 30, and 40 min of irradiation corresponding to 126, 189 and 252 J/cm energy fluences. For AlPcS+Au/Ag-NPs treatment, the viable count reduction were equal to 99.90, 99.96, and 99.975%, respectively. These results were significantly better than those accomplished for irradiated separated assays of AlPcS and Au/Ag-NPs.

Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review

“…One of the strategies for improved PS delivery involves the use of nanoparticles which are co-administered to allow the PS entry across the membrane or for a synergistic ROS response resulting in an antimicrobial action. A combination of tetrasulfonated hydroxyl aluminum phthalocyanine (AlPcS 4 ) and bimetallic gold/silver nanoparticles (Au/Ag-NPs) synthesized using a cell-free filtrate of Aureobasidium pullulans showed significantly higher killing as compared to the agents used individually (Maliszewska et al, 2018). Au/Ag-NPs possibly disrupted the cell membrane allowing enhanced uptake of the PS, AlPcS 4 .…”

On the Synergism of Biogenic Gold Nanoparticles and Hydroxyaluminum Phthalocyanines in the Photoeradication of Staphylococcus aureus

“…It is believed that the reduction in the membrane permeability barrier for lipophilic molecules results in a faster and higher uptake of the less sulfonated phthalocyanine than the more sulfonated derivative [ 26 ]. Taking into account our previous results that AlPcS 4 up to a concentration of 10 µg/mL is non-toxic to normal human keratinocytes (KERTr) and normal endothelial cells (HUVEC) [ 29 ], an attempt was made to increase the photo-bactericidal effect of di- and tetrasulfonated hydroxyaluminum phthalocyanines at a concentration of 7 µg/mL. Therefore, in the next set of our experiments, mixtures of hydroxyaluminum phthalocyanines and biogenic nanoparticles were used as photosensitizers.…”

Applications of Antimicrobial Photodynamic Therapy in Aquaculture: Effect on Fish Pathogenic Bacteria

“…Toluidine blue-carbon nanotube conjugate [41], Radachlorin [42], methylene blue [43,44], methylene blue-polymyxin conjugate [45], Temoporfin [44], prodigiosin [46], curcumin [19,47], Rose Bengal alone and when combined with methicillin [48], metal phthalocyanines [M = Zn(II), Cu (II), Co(II), In(III), and Lu (III)] bearing chlorine and dipentylmalonyl groups on peripheral positions and hexyloxy groups on non-peripheral positions [49], tetrasulfonated hydroxyaluminum phthalocyanine [50], zinc phthalocyanine-colistin conjugate [51], meso-tetra(3-N-methylpyridyl)porphyrin and meso-tetra(4-N-methylpyridyl)porphyrin [52], isomeric meso-tetra (pyridyl)-substituted porphyrin (5,10,15,20-Tetra(4-pyridyl)porphyrin (TPyP)) derivatives of cisplatin (3-cis-Pt-TPyP and 4-cis-Pt-TPyP) [16], dimethyl- [67,68], curcumin [69] Microalgae feed [67] water [68] Vibrio cholerae Gn Curcumin [69] Water [69] Vibrio fischeri Gn 5,10,15-tris(1-methylpyridinium-4-yl)-20-(pentafluorophenyl)porphyrin tri-iodide (Tri-Py+-Me-PF) [70] aquaculture water [70] Vibrio harveyi Gn Rose Bengal [71], curcumin [69] larviculture systems [71], water [69] Vibrio parahemolyticus Gn methylene blue [72], curcumin [36,73], 5,10,15-tris(1-methylpyridinium4-yl)-20-pentafluorophenylporphyrin tri-iodide (Tri-Py+-Me-PF) [35], 5,10,15,20-Tetrakis[N-methyl-4-pyridyl)porphyrin and 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin [74] Aquaculture water [35,74] Vibrio splendidus Gn Curcumin, methylene blue and eosin Y …”