Enhancement of photoassembly of the functionally active water-oxidizing complex in Mn-depleted photosystem II membranes upon transition to anaerobic conditions

Khorobrykh, A. A.; Yanykin, Denis V.; Klimov, Vyacheslav V.

doi:10.1016/j.jphotobiol.2016.08.028

Cited by 4 publications

(2 citation statements)

References 31 publications

(47 reference statements)

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“…The experiments on the photoassembly of the Mn 4 CaO 5 cluster were mainly carried out on isolated PSII membranes lacking a Mn 4 CaO 5 cluster. Although the photoassembly of the Mn 4 CaO 5 cluster in these cases may be different from that in vivo, in vitro studies provided a wide range of information regarding the cofactors involved in the photoassembly of the Mn 4 CaO 5 cluster [14][15][16][17][18][19][20][21][22][23][24][25][26][27]. A detailed description of the PSII photoactivation is presented in the reviews in [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Effect of Osmolytes on Photoassembly of Functionally Active Mn4CaO5 Cluster in Mn-Depleted Photosystem II Preparations Isolated from Spinach Leaves

Yanykin,

Kazantseva,

Khorobrykh

2023

Horticulturae

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The effect of osmolytes (trehalose, sucrose, betaine, D-glucose and hydroxyectoine) on the photoassembly of the functionally active inorganic core of the water-oxidizing complex (Mn4CaO5 cluster) in Mn-depleted PSII preparations (apo-WOC-PSII) was investigated. It was revealed that the efficiency of the photoassembly of the Mn4CaO5 cluster was considerably (three times) increased in the presence of 1 M disaccharides (trehalose or sucrose) in contrast to other osmolytes. It was found that the osmolytes (especially trehalose or sucrose) improved the redox interaction of exogenous Mn2+ with apo-WOC-PSII, enhanced the protective effect of Mn2+ against the photoinhibition of apo-WOC-PSII, protected CaCl2-treated PSII preparations against thermoinactivation, and stabilized the water-oxidizing complex and electron transport from QA to QB in native PSII preparations during heat treatment. It is suggested that the ability of osmolytes to enhance the efficiency of the photoassembly of a Mn4CaO5 cluster depends on their effect on the following key processes: the redox interaction of Mn2+ with apo-WOC-PSII; the stability of apo-WOC-PSII to photoinhibition during the photoactivation procedure; and the stabilization of both the newly assembled functionally active Mn4CaO5 cluster and the electron transfer from QA to QB.

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Section: Introductionmentioning

confidence: 99%

Effect of Osmolytes on Photoassembly of Functionally Active Mn4CaO5 Cluster in Mn-Depleted Photosystem II Preparations Isolated from Spinach Leaves

Yanykin,

Kazantseva,

Khorobrykh

2023

Horticulturae

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“…In the last three decades, these questions have been partly addressed in experiments on photoactivation of PSII, i.e. reconstitution of the Mn 4 CaO 5 cluster in WOC‐depleted PSII complexes (Blubaugh and Cheniae , Krieger et al , Rova et al , Büchel et al , Magnuson et al , Dasgupta et al , Khorobrykh et al ). However, to our knowledge, up to now, these important questions have not been systematically studied from the perspective of chloroplast biogenesis, when de novo assembly and functional development of the ‘newly born’ PSII complexes occurs in parallel with the maturation of the thylakoid membrane and the establishment of the entire ETC of the photosynthesis apparatus.…”

Section: Introductionmentioning

confidence: 99%

‘Birth defects’ of photosystem II make it highly susceptible to photodamage during chloroplast biogenesis

Shevela

Ananyev

Vatland

et al. 2019

Physiologia Plantarum

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High solar flux is known to diminish photosynthetic growth rates, reducing biomass productivity and lowering disease tolerance. Photosystem II (PSII) of plants is susceptible to photodamage (also known as photoinactivation) in strong light, resulting in severe loss of water oxidation capacity and destruction of the water‐oxidizing complex (WOC). The repair of damaged PSIIs comes at a high energy cost and requires de novo biosynthesis of damaged PSII subunits, reassembly of the WOC inorganic cofactors and membrane remodeling. Employing membrane‐inlet mass spectrometry and O2‐polarography under flashing light conditions, we demonstrate that newly synthesized PSII complexes are far more susceptible to photodamage than are mature PSII complexes. We examined these ‘PSII birth defects’ in barley seedlings and plastids (etiochloroplasts and chloroplasts) isolated at various times during de‐etiolation as chloroplast development begins and matures in synchronization with thylakoid membrane biogenesis and grana membrane formation. We show that the degree of PSII photodamage decreases simultaneously with biogenesis of the PSII turnover efficiency measured by O2‐polarography, and with grana membrane stacking, as determined by electron microscopy. Our data from fluorescence, QB‐inhibitor binding, and thermoluminescence studies indicate that the decline of the high‐light susceptibility of PSII to photodamage is coincident with appearance of electron transfer capability QA− → QB during de‐etiolation. This rate depends in turn on the downstream clearing of electrons upon buildup of the complete linear electron transfer chain and the formation of stacked grana membranes capable of longer‐range energy transfer.

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A possible relationship between the effect of factors on photoactivation of photosystem II depleted of functional Mn and cytochrome b559

Khorobrykh¹

2023

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Enhancement of photoassembly of the functionally active water-oxidizing complex in Mn-depleted photosystem II membranes upon transition to anaerobic conditions

Cited by 4 publications

References 31 publications

Effect of Osmolytes on Photoassembly of Functionally Active Mn4CaO5 Cluster in Mn-Depleted Photosystem II Preparations Isolated from Spinach Leaves

Effect of Osmolytes on Photoassembly of Functionally Active Mn4CaO5 Cluster in Mn-Depleted Photosystem II Preparations Isolated from Spinach Leaves

‘Birth defects’ of photosystem II make it highly susceptible to photodamage during chloroplast biogenesis

A possible relationship between the effect of factors on photoactivation of photosystem II depleted of functional Mn and cytochrome b559

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