Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro

Labanauskiene, J.; Šatkauskas, Saulius; Kirvelienė, Vida; Venslauskas, Mindaugas Saulius; Atkočius, Vydmantas; Didžiapetrienė, Janina

doi:10.3390/medicina45050047

Cited by 14 publications

(11 citation statements)

References 17 publications

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“…The electro-photodynamic therapy applied to lung cancer cells (A549) revealed a significant increase of the cytotoxic effect on cells when compared to HpD-PDT alone, even at drug doses significantly lower than typical levels. The results also demonstrated that the drug was delivered within minutes, instead of hours as in standard PDT (Skolucka et al 2011). Labanauskiene et al (2009 examined Chinese hamster lung fibroblast cell line (DC-3F) (2007) and murine hepatoma MH22A cells.…”

Section: Discussionmentioning

confidence: 54%

Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX)

Kulbacka¹,

Kotulska

Rembiałkowska³

et al. 2013

Cell Stress and Chaperones

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Two porphyrins, CoTPPS and MnTMPyPCl 5 , were tested for their photodynamic activity and potential novel use in a therapy of human cancers. We investigated an effect of photodynamic reaction (PDR), electroporation (EP) and their combination (electro-photodynamic reaction [EP-PDR]) on human colon adenocarcinoma cell lines (LoVo and resistant to doxorubicin LoVoDX), human breast adenocarcinoma (wild type MCF-7/WT and resistant to doxorubicin MCF-7/DOX), and human melanoma (Me45). The efficiency of macromolecules transport was examined with cytofluorymetry by assessing the degree of propidium iodide (PI) penetration. Additionally, cellular ultrastructure after EP was evaluated. We determined cyto-and photo-cytotoxic effect on the cells viability (MTT assay) after standard PDR and PDR combined with EP. Intracellular distribution and mitochondrial colocalization of both porphyrins was also performed. The experiments proved that both complexes exhibit desirable photodynamic properties on LoVo LoVoDX cells, and EP effectively supports photodynamic method in this type of cancer. The application of EP provided shorter time of incubation (only 10 min) and enhanced effect of applied therapy. The porphyrins did not affect the MCF-7 and Me45 cell lines.

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Section: Discussionmentioning

confidence: 54%

Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX)

Kulbacka¹,

Kotulska

Rembiałkowska³

et al. 2013

Cell Stress and Chaperones

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“…Soon after acknowledging of electroporation as a tool for targeted molecule delivery, it was exploited for intracellular transfer of anticancer drugs and DNA (Kinosita and Tsong 1978;Neumann et al 1982;Mir et al 1988;Labanauskiene et al 2009;Vásquez et al 2012). The feasibility of electroporation to enhance delivery of anticancer drugs into cells and tissues made possible the development of antitumor electrochemotherapy (ECT) , lately optimized in in vivo studies (Serša et al 1995;Miklavčič et al 1998;Š atkauskas et al 2005;Soden et al 2006;Corovic et al 2008;Labanauskien_ e et al 2009) and implementation in clinics (Belehradek et al 1993;Mir et al 2006;Edhemovic et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro

et al. 2015

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The aim of this study was to compare different and commonly used cell viability assays after CHO cells treatment with anticancer drug bleomycin (20 nM), high voltage (HV) electric pulses (4 pulses, 1200 V/cm, 100 µs, 1 Hz), and combination of bleomycin and HV electric pulses. Cell viability was measured using clonogenic assay, propidium iodide (PI) assay, MTT assay, and employing flow cytometry modality to precisely count cells in definite volume of the sample (flow cytometry assay). Results showed that although clonogenic cell viability drastically decreased correspondingly to 57 and 3 % after cell treatment either with HV pulses or combination of bleomycin and HV pulses (bleomycin electrotransfer), PI assay performed ~15 min after the treatments indicated nearly 100 % cell viability. MTT assay performed at 6-72 h time points after these treatments revealed that MTT cell viability is highly dependent on evaluation time point and decreased with later evaluation time points. Nevertheless, in comparison to clonogenic cell viability, MTT cell viability after bleomycin electrotransfer at all testing time points was significantly higher. Flow cytometry assay if used at later times, 2-3 days after the treatment, allowed reliable evaluation of cell viability. In overall, our results showed that in order to estimate cell viability after cell treatment with combination of the bleomycin and electroporation the most reliable method is clonogenic assay. Improper use of PI and MTT assays can lead to misinterpretation of the experimental results.

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“…Primary manipulation of the target tissue with electroporation, hyperoxygenation, treatment with PDT sensitizers and/or hyperthermia have been shown to increase the effectiveness of PDT [57][58][59][60]. Also, work is being done to increase the depth of penetration for PDT by designing photosensitizers that can be directly activated by infrared or nearinfrared light, wavelengths that can penetrate very deeply into tissue, or by creating constructs that include upconverting nanophosphors that can absorb light in these wavelengths and emit the activating visible light [61][62][63][64][65].…”

Section: Five-year Viewmentioning

confidence: 99%

Photodynamic therapy for malignant pleural mesothelioma: the future of treatment?

Friedberg

2011

Expert Review of Respiratory Medicine

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Malignant pleural mesothelioma is a deadly incurable cancer, with a median survival of approximately 9 months. The best available chemotherapy, arguably the standard of care, only yields a 40% response rate and an 11-week extension in median survival. Surgery, the modality most likely to be associated with prolonged remission, remains investigational and must always be combined with other modalities in an effort to treat the microscopic disease that will remain even after the most aggressive operations. One such modality, photodynamic therapy, is a light-based cancer treatment that has features making it particularly well suited as a component of a surgery-based multimodal treatment plan. Utilizing intraoperative photodynamic therapy has enabled development of a less drastic surgical procedure that is also yielding some encouraging survival results. A unique aspect of photodynamic therapy is its stimulation of a tumor-directed immune response, a feature that offers promise for designing future treatments.

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Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro

Abstract: Summary. The aim of our study was to determine if electroporation could improve the effi-Medicina (Kaunas) 2009; 45(5) Correspondence to J.

Cited by 14 publications

References 17 publications

Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX)

Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX)

Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro

Photodynamic therapy for malignant pleural mesothelioma: the future of treatment?

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