Enhancement of photorespiration in immobilized Chlamydomonas reinhardtii cells

Garbayo, Inés; Forján, Eduardo; Salguero, A.; Cuaresma, María; Vega, JoséM.; Vílchez, Carlos

doi:10.1007/s10529-004-8352-9

Cited by 11 publications

(8 citation statements)

References 10 publications

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“…Dissolved O 2 induces photorespiration for green algae, which lessens growth rate [35] due to an oxygenation action of the ribulose-1,5-bisphosphate carboxylase/oxygenase, RubisCO, in the Calvin-Benson cycle [36]. Our observation supports the photorespiration behavior as shown in Fig.…”

Section: Supercritical Fluid Processing Of Samplessupporting

confidence: 88%

Carotenoid production from Chlorococcum littorale in photoautotrophic cultures with downstream supercritical fluid processing

Ota

Watanabe

Kato

et al. 2009

J of Separation Science

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Carotenoid production from highly CO(2 )tolerant microalga Chlorococcum littorale in photoautotrophic cultures with downstream supercritical fluid processing was studied. Increasing temperature, increasing light intensity and decreasing CO(2) and O(2) gas concentrations enhanced growth rate under nitrate-rich conditions. Carotenoid content was insensitive to nitrate concentration, temperature and gas composition, but was greatly promoted by light intensity. Growth rate and carotenoid content had an optimum light intensity of ca. 120 micromol-photon * m(-2)s(-1). Separation of two sample cultures was studied by applying supercritical fluid extraction with CO(2 )and 10 mol% ethanol co-solvent. Extraction yield of carotenoids was 90% with 10 mol% ethanol at 333 K and 30 MPa. Selectivity of a sample with less lipid content (12.9 wt%) was five-fold higher than that with higher lipid content (29.4 w%).

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Section: Supercritical Fluid Processing Of Samplessupporting

confidence: 88%

Carotenoid production from Chlorococcum littorale in photoautotrophic cultures with downstream supercritical fluid processing

Ota

Watanabe

Kato

et al. 2009

J of Separation Science

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“…In higher plants, the inactivation of the C 2 -cycle leads to changes in the carbon allocation pattern and to an earlier senescence. However, in some green algae, like Chlamydomonas, the inactivation of the C 2 -cycle does not induce glycolate accumulation inside the cells because of an active excretion of glycolate (Garbayo et al, 2005;V ılchez et al, 1991). For a biotechnological use of glycolate excretion by algal cells, a high glycolate concentration in the surrounding medium is required (see below).…”

Section: Introductionmentioning

confidence: 99%

Glycolate from microalgae: an efficient carbon source for biotechnological applications

Taubert

Jakob

Wilhelm

2019

Plant Biotechnology Journal

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Summary Glycolate is produced in autotrophic cells under high temperatures and C i ‐limitation via oxygenation of ribulose‐1,5‐bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C 2 cycle by consuming reductants, ATP and CO 2 emission (photorespiration). Therefore, photorespiration is an inhibitory process for biomass production. However, cells can be manipulated in a way that they become glycolate‐producing ‘cell factories’, when the ratio carboxylation/oxygenation is 2. If under these conditions the C 2 cycle is blocked, glycolate excretion becomes the only pathway of photosynthetic carbon flow. The study aims to proof the biotechnological applicability of algal‐based glycolate excretion as a new biotechnological platform. It is shown that cells of Chlamydomonas can be cultivated under specific conditions to establish a constant and long‐term stable glycolate excretion during the light phase. The cultures achieved a high efficiency of 82% of assimilated carbon transferred into glycolate biosynthesis without losses of function in cell vitality. Moreover, the glycolate accumulation in the medium is high enough to be directly used for microbial fermentation but does not show toxic effects to the glycolate‐producing cells.

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“…The material is cheap and can be produced in industrial quantities from a renewable resource. However, alginate polymers have low mechanical stability, and microbial cells are usually immobilized in alginate as beads (Dainty et al, 1986;Garbayo et al, 2005;Vilchez et al, 2001). Unfortunately, the entrapment of cells in alginate beads is not a good option for phototrophic algae, because of poor light-utilization efficiency.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen photoproduction by nutrient‐deprived Chlamydomonas reinhardtii cells immobilized within thin alginate films under aerobic and anaerobic conditions

Kosourov

Seibert

2008

Biotech & Bioengineering

168

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A new technique for immobilizing H2-photoproducing green algae within a thin (<400 microm) alginate film has been developed. Alginate films with entrapped sulfur/phosphorus-deprived Chlamydomonas reinhardtii, strain cc124, cells demonstrate (a) higher cell density (up to 2,000 microg Chl mL(-1) of matrix), (b) kinetics of H2 photoproduction similar to sulfur-deprived suspension cultures, (c) higher specific rates (up to 12.5 micromol mg(-1) Chl h(-1)) of H2 evolution, (d) light conversion efficiencies to H2 of over 1% and (e) unexpectedly high resistance of the H2-photoproducing system to inactivation by atmospheric O2. The algal cells, entrapped in alginate and then placed in vials containing 21% O2 in the headspace, evolved up to 67% of the H2 gas produced under anaerobic conditions. The results indicate that the lower susceptibility of the immobilized algal H2-producing system to inactivation by O2 depends on two factors: (a) the presence of acetate in the medium, which supports higher rates of respiration and (b) the capability of the alginate polymer itself to effectively separate the entrapped cells from O2 in the liquid and headspace and restrict O2 diffusion into the matrix. The strategy presented for immobilizing algal cells within thin polymeric matrices shows the potential for scale-up and possible future applications.

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Enhancement of photorespiration in immobilized Chlamydomonas reinhardtii cells

Cited by 11 publications

References 10 publications

Carotenoid production from Chlorococcum littorale in photoautotrophic cultures with downstream supercritical fluid processing

Carotenoid production from Chlorococcum littorale in photoautotrophic cultures with downstream supercritical fluid processing

Glycolate from microalgae: an efficient carbon source for biotechnological applications

Hydrogen photoproduction by nutrient‐deprived Chlamydomonas reinhardtii cells immobilized within thin alginate films under aerobic and anaerobic conditions

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