Enhancement of photosynthetic O2 evolution in Chlorella vulgaris under high light and increased CO2 concentration as a sign of acclimation to phosphate deficiency

Kozlowska-Szerenos, B.; Bialuk, Izabela; Maleszewski, S.

doi:10.1016/j.plaphy.2004.02.010

Cited by 40 publications

(18 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As is visible in Table I, the affinity for C i had already increased after 3 h in all of the three phosphate concentrations. The maximal rate of oxygen evolution was lower in low-CO 2 -grown compared with high-CO 2 -grown cells, but there was no difference between cultures with different concentrations of phosphate, indicating that none of the concentrations of phosphate were limiting (Kozlowska-Szerenos et al, 2004;Beardall et al, 2005a). In summary, both the induction of mtCA as well as the decreased K m values indicate that CCM was at least partly induced already after 3 h in low CO 2 .…”

Section: Resultsmentioning

confidence: 77%

A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii

et al. 2010

View full text Add to dashboard Cite

(J.A.R.) Using a gas chromatography-mass spectrometry-time of flight technique, we determined major metabolite changes during induction of the carbon-concentrating mechanism in the unicellular green alga Chlamydomonas reinhardtii. In total, 128 metabolites with significant differences between high-and low-CO 2 -grown cells were detected, of which 82 were wholly or partially identified, including amino acids, lipids, and carbohydrates. In a 24-h time course experiment, we show that the amino acids serine and phenylalanine increase transiently while aspartate and glutamate decrease after transfer to low CO 2 . The biggest differences were typically observed 3 h after transfer to low-CO 2 conditions. Therefore, we made a careful metabolomic examination at the 3-h time point, comparing low-CO 2 treatment to high-CO 2 control. Five metabolites involved in photorespiration, 11 amino acids, and one lipid were increased, while six amino acids and, interestingly, 21 lipids were significantly lower. Our conclusion is that the metabolic pattern during early induction of the carbon-concentrating mechanism fit a model where photorespiration is increasing.

show abstract

Section: Resultsmentioning

confidence: 77%

A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii

et al. 2010

View full text Add to dashboard Cite

show abstract

“…It has been demonstrated that the effect of phosphate on carbon metabolism may depend also on other factors, especially on irradiance and CO 2 concentration. The photosynthetic oxygen evolution in the phosphorus-deficient cells was significantly higher than the control under high irradiance and adequate inorganic carbon supply (Kozłowska-Szerenos et al 2004), which could soften some harmful effects of phosphate deficiency. In addition, phosphate-deficient cultures demonstrated an enhanced nitrate reduction (Kozłowska-Szerenos et al 2004), and improved nitrate assimilation could increase chlorophyll and Rubisco synthesis.…”

Section: Resultsmentioning

confidence: 85%

“…The photosynthetic oxygen evolution in the phosphorus-deficient cells was significantly higher than the control under high irradiance and adequate inorganic carbon supply (Kozłowska-Szerenos et al 2004), which could soften some harmful effects of phosphate deficiency. In addition, phosphate-deficient cultures demonstrated an enhanced nitrate reduction (Kozłowska-Szerenos et al 2004), and improved nitrate assimilation could increase chlorophyll and Rubisco synthesis. Therefore, it could be justified that the growth was not inhibited by the low phosphate level in the present investigation.…”

Section: Resultsmentioning

confidence: 85%

“…Since carbon acquisition in most microalgae is energy dependent, requiring ATP, it might be expected that phosphate limitation could have an indirect regulatory influence on CCM activity (Beardall et al 1998). The CCM activity of Chlorella emersonii was down-regulated by phosphate limitation , however, phosphate deficiency increased the affinity for external inorganic carbon (1/K 0.5 ) in photosynthesis of Chlorella vulgaris at high irradiance (Kozłowska-Szerenos et al 2000, 2004.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of inorganic carbon acquisition by nitrogen and phosphorus levels in the Nannochloropsis sp.

Zhou

2009

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Nannochloropsis sp. was grown to the exponential phase and transferred to the high CO 2 (2,800 ll l -1 ) and irradiance (100 lmol photons m -2 s -1 ) condition with different levels of nitrate and phosphate for 72 h, then the photosynthetic activity and inorganic carbon acquisition of the alga were measured. The apparent photosynthetic efficiency (a) of Nannochloropsis sp. decreased with increasing NO 3 -concentration from 150 to 3,000 lM, and the high nitrate-grown cells showed the lowest levels of light-saturated photosynthetic rate (P m ), while the low nitrate-grown cells showed the highest levels of dark respiration rate (R d ). The maximal light-saturated photosynthetic rate and the minimal dark respiration rate were seen under the middle nitrate condition. When the nitrate concentration ranged from 150 to 3,000 lM, the affinity for inorganic carbons of Nannochloropsis sp. increased sharply with the increasing NO 3 -concentration to 300 lM and then decreased significantly. The middle phosphate-grown cells exhibited the highest light-saturated photosynthetic rate and apparent photosynthetic efficiency, however, the affinity for inorganic carbons of Nannochloropsis sp. was the maximum under the low phosphate condition. It was shown that the appropriate nitrogen and phosphorus levels were of vital importance to the photosynthesis of cells.

show abstract

“…They utilized more of the absorbed PAR energy for nitrate reduction than the control cells. Under the conditions of more efficient CO 2 supply (high inorganic carbon concentration, the presence of exogenous carbonic anhydrase in the cell suspension) and at a high irradiance the net photosynthetic O 2 evolution of cells from -P cultures reached even higher rate than in the controls (Kozłowska-Szerenos et al 2004). …”

Section: Introductionmentioning

confidence: 96%

Photosynthetic net O<sub>2</sub> evolution enhancement as a sign of acclimation to phosphorus deficiency in bean (Phaseolus vulgaris L.) leaves

Kozlowska-Szerenos¹,

Jarosz²,

Maleszewski³

2009

Photosynt.

View full text Add to dashboard Cite

Primary leaves of bean (Phaseolus vulgaris L.) seedlings cultivated for 14 days in a growth chamber on complete (control) and phosphate deficient (-P) Knop liquid medium were used for measurements. The -P leaves were smaller and showed an increased specific leaf area (SLA). Their inorganic phosphate (P i ) concentration was considerably lowered. They did not show any significant changes in chlorophyll (Chl) (a + b) concentration and in their net CO 2 assimilation rate when it was estimated under the conditions close to those of the seedlings growth. Light response curves of photosynthetic net O 2 evolution (P N O 2 ) of the leaves for the irradiation range up to 500 µmol(photon) m -2 s -1 were determined, using the leaf-disc Clark oxygen electrode. The measurements were taken under high CO 2 concentration of about 1 % and O 2 concentrations of 21 % or lowered to about 3 % at the beginning of measurement. The results obtained at 21 % O 2 and the irradiations close to or higher than those used during the seedlings growth revealed the phosphorus stress suppressive effect on the leaf net O 2 evolution, however, no such effect was observed at lower irradiations. Other estimated parameters of P N O 2 such as: apparent quantum requirement (QR A ) and light compensation point (LCP) for the control and -P leaves were similar. However, with a high irradiation and lowered O 2 concentration the rate of P N O 2 for the -P leaves was markedly higher than that for the control, in relation to both the leaf area and leaf fresh mass. This difference also disappeared at low irradiations, but the estimated reduced QR A values indicate, under those conditions, the increased yield of photosynthetic light reaction, especially in the -P leaves. The presented results confirm the suggestion that during the initial phase of insufficient phosphate feeding the acclimations in the light phase of photosynthesis, both structural and functional appear. They correspond, probably, to the increased energy costs of carbon assimilation under phosphorus stress, e.g. connected with raised difficulties in phosphate uptake and turnover and enhanced photorespiration. Under the experimental conditions especially advantageous for the dark phase of photosynthesis (saturating CO 2 and PAR, low O 2 concentration), those acclimations may be manifested as an enhancement of photosynthetic net O 2 evolution.

show abstract

Enhancement of photosynthetic O2 evolution in Chlorella vulgaris under high light and increased CO2 concentration as a sign of acclimation to phosphate deficiency

Cited by 40 publications

References 39 publications

A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii

A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii

Regulation of inorganic carbon acquisition by nitrogen and phosphorus levels in the Nannochloropsis sp.

Photosynthetic net O<sub>2</sub> evolution enhancement as a sign of acclimation to phosphorus deficiency in bean (Phaseolus vulgaris L.) leaves

Contact Info

Product

Resources

About