Enhancement of plant regeneration from embryogenic callus of commercial barley cultivars

Bregitzer, Phil; Dahleen, Lynn S.; Campbell, Robert D.

doi:10.1007/s002990050514

Cited by 47 publications

(52 citation statements)

References 13 publications

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“…The results of this study, showed that it is possible to get regeneration from plumule and radicle explants of wheat and confirmed that the shoot regeneration is considerably affected by the type of cultivar, concentration of plant growth regulator and explant used in the study in agreement with Bregitzer et al (1998). The results are partially supported by the studies of Balli et al (1993), in which a maximum regeneration rate occurred with the addition of 2.5 mg/L of 2.4-D in the regeneration medium.…”

Section: Discussionsupporting

confidence: 74%

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

Kahriz¹,

Kahriz²

2017

JSM

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Section: Discussionsupporting

confidence: 74%

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

Kahriz¹,

Kahriz²

2017

JSM

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“…It has been reported that the formation of embryogenic callus in various barley cultivars was depended on 2,4-D concentration, and 2-3 mg/L was adequate in most cases (Bregitzer et al, 1998;Zapata et al, 2004). However, it has been further demonstrated that for cv.…”

Section: Discussionmentioning

confidence: 98%

“…Despite the fact that barley presents as a promising model plant for the development methods of genetic engineering, its transformation is marked by some complications. For barley, the protocols for callus induction and plant regeneration are not efficiently and widely used because of high genotype-specific dependency (Taniguchi et al, 1991;Akula et al, 1999;Ganeshan et al, 2003) and low regeneration efficiency (Rengel, 1987;Bregitzer et al, 1998;Chen et al, 2006). Therefore, the application of genetic engineering has been restricted to only a few barley germplasms that possess high tissue culture capability; the so-called model cultivars mostly contain a low agronomic value.…”

Section: Introductionmentioning

confidence: 99%

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Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgare L.)

Han

Jin

et al. 2011

J. Zhejiang Univ. Sci. B

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An efficient induction system and regeneration protocol based on mature barley embryos were developed. Embryos isolated from mature seeds, dehusked by hand and inoculated with longitudinally bisected sections, showed low contamination and high primary callus-forming capability. The influences of nine culture media on primary callus induction and germination from the mature embryos of barley cultivars Golden Promise and Zaoshu 3 were analyzed. The results showed that the two cultivars had much higher values of primary callus induction in the B16M6D medium as compared to the other eight medium formulations, with a frequency of 74.3% and 78.4% for Golden Promise and Zaoshu 3, respectively. Furthermore, Zaoshu 3 demonstrated particularly high stability in callus induction over the different media, indicating its potential utilization in callus induction and regeneration for its good agronomic traits and wide adaption. There were significant differences amongst 11 barley genotypes in terms of primary callus induction in the optimum medium, with percentages of callus induction and germination response ranging from 17.9% to 78.4% and 2.8% to 47.4%, respectively. Green plantlets of Dong 17, Golden Promise, and Zaoshu 3 were successfully developed from primary calli through embryogenesis, with green plant differentiation frequencies ranging from 9.7% to 21.0% across genotypes.

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“…Similarly, Walmsley et al (1995) optimized culture conditions for Australian barley cultivars using immature embryos and noted percent embryos producing calli, somatic embryos per zygotic embryo and shoots per zygotic embryo. On the other hand, Bregitzer et al (1998) considered parameters such as callus fresh weight, green plants per embryo, green plants per gram fresh weight and plants per plate to assess plant regeneration efficiency for commercial barley cultivars. Barro et al (1999) used the terms "embryogenic capacity" and "regeneration capacity" for the number of explants producing embryogenic callus and the number of calli producing shoots/number of explants, respectively.…”

Section: Mots Clésmentioning

confidence: 99%

Evaluation of the Enhanced Regeneration System for in vitro regeneration in barley

Ganeshan¹,

Weir²,

Båga³

et al. 2006

Can. J. Plant Sci.

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. Evaluation of the Enhanced Regeneration System for in vitro regeneration in barley. Can. J. Plant Sci. 86: 63-69. A simple two-step model for evaluation of in vitro regeneration protocols is proposed based on callus induction and regeneration from immature scutella of two Canadian barley (Hordeum vulgare L.) genotypes, AC Metcalfe and SB92559 using the Enhanced Regeneration System (ERS). The number of explants producing embryogenic callus, the number of plants per embryogenic callus and the number of plants per explant were considered. Tissue culture parameters included three combinations of growth regulators, two carbon sources in culture media, and three cold treatment regimes of spikes prior to scutella isolation. Culture medium containing 5 µM 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 µM benzyl adenine (BA) induced the highest percent of embryogenic calli and the highest number of shoots per embryogenic callus from AC Metcalfe. Medium containing 3.75 µM 2,4-D and 0.75 µM BA gave the best response for SB92559. Both genotypes produced more shoots on maltose than on sucrose medium. A 2-d treatment of spikes at 4°C resulted in best response for SB92559. Regeneration response from AC Metcalfe scutella from spikes was unaffected by being subjected to 2, 4 or 6 d of cold. Conditions resulting in best responses from both genotypes were tested on four commercial barley varieties. However, these lines showed inferior regeneration compared to SB92559 and AC Metcalfe. Les deux génotypes ont produit plus de pousses sur le milieu à base de maltose que sur celui à base de sucrose. Le conditionnement de l'épi à 4°C pendant deux jours a suscité la meilleure réaction chez SB92559. La régénération à partir des écussons prélevés sur les épis d'AC Metcalfe n'a pas été affectée par un conditionnement au froid de deux, quatre ou six jours. Les paramètres engendrant la meilleure réaction chez les deux géno-types ont ensuite été testés sur quatre variétés commerciales d'orge. Malheureusement, ces lignées ont donné de moins bons résul-tats que SB92559 et AC Metcalfe sur le plan de la régénération.

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Enhancement of plant regeneration from embryogenic callus of commercial barley cultivars

Cited by 47 publications

References 13 publications

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgare L.)

Evaluation of the Enhanced Regeneration System for in vitro regeneration in barley

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