Enhancement of poly(L-lactide)-degrading enzyme production by Laceyella sacchari LP175 using agricultural crops as substrates and its degradation of poly(L-lactide) polymer

Kitpreechavanich, Vichien; Hanphakphoom, Srisuda; Kongsaeree, Prachumporn T.; Srisuk, Nantana; Guicherd, Marie; Cioci, Gianluca; Duquesne, Sophie; Marty, Alain

doi:10.1016/j.polymdegradstab.2017.06.017

Cited by 12 publications

(25 citation statements)

References 21 publications

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“…Aeration rate was an important factor for cultivation of L. sacchari LP175 in the airlift fermenter as it improved the heat and mass transfer in the reactor. An airlift fermenter does not contain an agitator compared to a stirrer fermenter [13]. Nadeem et al [18] reported that the increased aeration rate improved oxygen transfer and nutrient mixing in the fermentation system.…”

Section: Resultsmentioning

confidence: 99%

“…Enzyme production in a 10.0 L airlift fermenter Serine protease production was carried out in a 10.0 L airlift fermenter using 7.0 L working volume of the optimized medium; 4.64 g/L cassava chips, 1.53 g/L soybean meal and 0.31 g/L PLA powder, pH 7.0 [13]. The fermentation was conducted at 50 °C with different aeration rates of 0.2 -1.0 vvm for 36 h. Protease activity was determined using 0.6 % (w/v) casein in 0.1 M Tris-HCl, pH 9.0 as substrate, modified from the method of Jarerat et al [16].…”

Section: Microorganismmentioning

confidence: 99%

“…Laceyella sacchari LP175 showed PLA-degrading enzyme production characterized as serine protease activity [11,12]. This utilized low-cost substrates for growth and enzyme production in a 3.0 L airlift fermenter, yielding 94.4 U/mL [13]. Crude enzyme obtained from L. sacchari LP175 degraded PLA polymer and poly(lactide)/thermoplastic starch blend film at 50 °C.…”

Section: Introductionmentioning

confidence: 99%

“…Each microorganism has optimal growth conditions. Aeration rate is a highly important factor in an airlift fermenter that does not contain an agitation system compared with a stirrer fermenter [12,13]. Thus, the effect of aeration rate on serine protease production by L. sacchari LP175 in a 10.0 L airlift fermenter was investigated.…”

Section: Introductionmentioning

confidence: 99%

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Degradation of Poly(Butylene Succinate) and Poly(Butylene Succinate)/Poly(Lactide) Blends using Serine Protease Produced from Laceyella sacchari LP175

et al. 2021

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The thermophilic filamentous bacterium Laceyella sacchari LP175 was cultivated in a 10.0 L airlift fermenter to produce serine protease at 50 °C. Maximal serine protease activity at 1,123.32 ± 15.8 U/mL was obtained for cultivation at 0.6 vvm aeration rate for 36 h. The crude enzyme was applied for degradation of poly (butylene succinate) (PBS), and poly (butylene succinate)/poly(lactide) blend (PBS/PLA) powders at 50 °C for 48 h with different substrates and enzyme concentrations. Results showed that serine protease produced from L. sacchari LP175 degraded PBS and PBS/PLA at 46.5 ± 2.05 and 49.8 ± 1.45 %, respectively, at an initial substrate concentration of 100 g/L with 1,200 U/mL of serine protease activity. Percentage degradation of PBS and PBS/PLA was improved to 51.4 ± 1.06 and 56.9 ± 1.42 %, respectively, when upscaled in a 2.0 L stirrer fermenter with 200 rpm agitation rate. Degradation products evaluated by a scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed that serine protease produced from L. sacchari LP175 degraded both PBS and PBS/PLA polymers. Results showed that microbial enzyme technology could be used to degrade PBS and PBS/PLA blend polymers and reduce the accumulation of waste. HIGHLIGHTS Upscaled serine protease production was achieved in a 10 L airlift fermenter by sacchari LP175 using low-cost agricultural products as substrate The crude enzyme degraded PBS and PBS/PLA powders (100 g/L) at up to 51.4 and 56.9 %, respectively in a 2.0 L stirrer fermenter under optimal conditions Degradation products of PBS and PBS/PLA by crude enzyme produced from sacchari LP175 were characterized GRAPHICAL ABSTRACT

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Section: Resultsmentioning

confidence: 99%

Section: Microorganismmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Degradation of Poly(Butylene Succinate) and Poly(Butylene Succinate)/Poly(Lactide) Blends using Serine Protease Produced from Laceyella sacchari LP175

et al. 2021

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“…As crosslinked hydrophilic polymers, superabsorbents have been widely used in many fields, such as agriculture [ 12 ], horticulture [ 13 ], drug delivery [ 14 ], sanitary goods [ 15 ], artificial snow [ 16 ], dye removal [ 17 ], and wastewater treatment [ 18 ]. Efforts have been focused on decreasing preparation cost, improving salt tolerance, and making the material environmentally degradable.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Applications of Salt-Resistant Superabsorbent Poly (Acrylic Acid-Acrylamide/Fly Ash) Composite

et al. 2019

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Solution polymerization synthesized alt-resistant superabsorbent poly (acrylic acid-acrylamide/fly ash) composites. The mass ratio of acrylic acid (AA) to acrylamide (AM), the concentration of crosslinker, the neutralization degree (ND) of AA, and the polymerization temperature were investigated by single-factor method. Optimized conditions for the synthesis of poly (acrylic acid-acrylamide/fly ash) (PAA-AM/FA) are, as following: m (AA)/m (AM) is 1.5, the content of crosslinker N, N-methylenebisacrylamide. (MBA) is 0.7%, neutralization degree of AA is 70%, polymerization temperature is 70 °C, and fly ash (FA) content is 50%. The prepared PAA-AM/FA demonstrated superior water absorption performance. The absorption capacities of PAA-AM/FA for pure water and 0.9% NaCl solution were found to be 976 g·g−1 and 81 g·g−1, respectively. Furthermore, PAA-AM/FA was found to have excellent adsorption capacity (148 mg·g−1) for Rhodamine B in water. Fourier Transform-Infrared Spectroscopy (FT-IR), Thermogravimetric Analysis (TGA), and Scanning Electron Microscopy (SEM) characterized the prepared materials. Results showed that fly ash was incorporated into the macromolecular polymer matrix and played a key role in improving the performance of the polymer composites.

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The Role of Interchain Force and/or Chain Entanglement in the Melt Strength and Ductility of PLA‐Based Materials

Chen

Sun

et al. 2023

Chemistry An Asian Journal

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As an eco‐friendly material, PLA was a desirable alternative to polyethylene and polypropylene films due to its biodegradability. The preferable melt strength of PLA‐based materials was a key factor in ensuring its processing using extrusion blow. This paper focuses on the influence of interchain force and/or chain entanglement on the melt strength and ductility of PLA‐based materials in recent years. In addition, the preparation of PLA‐based materials via physical blending or reactive processing was also summarized. The blending of PLA with a flexible heteropolymer, driven by the interchain force and/or chain entanglements, were characterized as a practicable method for toughening PLA‐based materials. Also, the restructuring of PLA chains, by branching based on chain entanglement, was suitable for increasing chain entanglements in PLA matrix, yielding satisfactory melt strength and ductility. This review aims to elucidate the relationship between interchain forces and/or entanglement with the melt strength and ductility of PLA‐based materials. An essential and systematic understanding of the tailoring melt strength and rheological properties of PLA by interchain forces and/or entanglement was apt to improve and perfect the processing technology of the extrusion blow, and consequently improve the tensile strength and toughness of PLA films.

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Enhancement of poly(L-lactide)-degrading enzyme production by Laceyella sacchari LP175 using agricultural crops as substrates and its degradation of poly(L-lactide) polymer

Cited by 12 publications

References 21 publications

Degradation of Poly(Butylene Succinate) and Poly(Butylene Succinate)/Poly(Lactide) Blends using Serine Protease Produced from Laceyella sacchari LP175

Degradation of Poly(Butylene Succinate) and Poly(Butylene Succinate)/Poly(Lactide) Blends using Serine Protease Produced from Laceyella sacchari LP175

Preparation and Applications of Salt-Resistant Superabsorbent Poly (Acrylic Acid-Acrylamide/Fly Ash) Composite

The Role of Interchain Force and/or Chain Entanglement in the Melt Strength and Ductility of PLA‐Based Materials

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