Enhancement of Polyunsaturated Fatty Acid Production by Tn5 Transposon in Shewanella baltica

Amiri-Jami, Mitra; Wang, Haifeng; Kakùda, Yukio; Griffiths, Mansel W.

doi:10.1007/s10529-006-9077-8

Cited by 24 publications

(13 citation statements)

References 19 publications

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“…A similar result was obtained using an EPA-producing deep-sea bacterium, Photobacterium profundum SS9 (Allen et al 1999). The enhancement of EPA production was also reported in cells of Shewanella baltica that had been mutated with transposon Tn5 (Amiri-Jami et al 2006). …”

Section: Introductionmentioning

confidence: 63%

Enhanced heterologous production of eicosapentaenoic acid in Escherichia coli cells that co-express eicosapentaenoic acid biosynthesis pfa genes and foreign DNA fragments including a high-performance catalase gene, vktA

Orikasa

Ito²,

Nishida

et al. 2007

Biotechnol Lett

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Cellular eicosapentaenoic acid (EPA) makes up approximately 3% of total fatty acids in Escherichia coli DH5α, a strain that carries EPA biosynthesis genes (pEPAΔ1). EPA was increased to 12% of total fatty acids when the host cell coexpressed the vector pGBM3::sa1(vktA), which carried the high-performance catalase gene, vktA. Where this vector was coexpressed, the transformant accumulated a large amount of VktA protein. However, the EPA production of cells carrying the vector that included the insert lacking almost the entire vktA gene was approximately 6%. This suggests that the retention of a large DNA insert in the vector and the accumulation of the resulting protein, but not the catalytic activity of VktA catalase, would potentially be able to increase the content of EPA.

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Section: Introductionmentioning

confidence: 63%

Enhanced heterologous production of eicosapentaenoic acid in Escherichia coli cells that co-express eicosapentaenoic acid biosynthesis pfa genes and foreign DNA fragments including a high-performance catalase gene, vktA

Orikasa

Ito²,

Nishida

et al. 2007

Biotechnol Lett

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“…The same phenomenon is reported for recombinant production of DHA in E. coli DH5a (Orikasa et al 2006b), when the level of DHA was increased by increasing the temperature to 15°C. As well, the recombinant production of EPA by E. coli EPI300T1 transformant No.5 does not correspond with that by S. baltica MAC1, in which the level of EPA and DHA increased with decreasing growth temperature (Amiri-Jami et al 2006). This suggests that the effect of temperature on EPA ⁄ DHA production in E. coli may not be controlled only by EPA ⁄ DHA biosynthesis enzymes but also by some other unknown factor(s).…”

Section: Discussionmentioning

confidence: 89%

“…2006b), when the level of DHA was increased by increasing the temperature to 15°C. As well, the recombinant production of EPA by E. coli EPI300T1 transformant No.5 does not correspond with that by S. baltica MAC1, in which the level of EPA and DHA increased with decreasing growth temperature (Amiri‐Jami et al. 2006).…”

Section: Discussionmentioning

confidence: 94%

Recombinant production of omega-3 fatty acids in Escherichia coli using a gene cluster isolated from Shewanella baltica MAC1

Amiri-Jami

Griffiths²

2010

Journal of Applied Microbiology

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Aim: To isolate eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) genes from Shewanella baltica MAC1 and to examine recombinant production of EPA and DHA in E. coli to investigate cost‐effective, sustainable and convenient alternative sources for fish oils. Methods and Results: A fosmid library was prepared from the genomic DNA of S. baltica MAC1 and was screened for EPA and DHA genes by colony hybridization using a partial fragment of the S. baltica MAC1 pfaA and pfaD genes as probes. Analysis of total fatty acids isolated from transgenic E. coli positive for pfaA and pfaD genes by gas chromatography and gas chromatography‐mass spectrometry indicated recombinant production of both EPA and DHA. Analysis of the complete nucleotide sequence for the isolated gene cluster showed 16 putative open reading frames (ORFs). Among those, four ORFs showed homology with pfaA, pfaB, pfaC and pfaD genes of the EPA and/or DHA biosynthesis gene clusters; however, the protein domains of these genes were different from other EPA/DHA biosynthesis genes. Conclusions: The EPA and DHA gene cluster was cloned successfully. The transgenic E. coli strain carrying the omega‐3 gene cluster was able to produce both EPA and DHA. The isolated gene cluster contained all the genes required for the recombinant production of both EPA and DHA in E. coli. Significance and Impact of the Study: These findings have implications for any future use of the EPA and DHA gene cluster in other micro‐organisms, notably those being used for fermentation. Recombinant production of both EPA and DHA by E. coli or any other micro‐organism has great potential to add economic value to a variety of industrial and agricultural products.

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“…Selection-based strain improvement, often enabled by random mutagenesis, has been very successful for the production of carboxylic acids (5, 7). However, our ability to produce carboxylic acids and other fermentation products is often limited by complex cellular metabolism and regulations (20).…”

Section: Introductionmentioning

confidence: 99%

Metabolic Engineering of Biocatalysts for Carboxylic Acids Production

Liu

Jarboe

2012

Computational and Structural Biotechnology Journal

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Fermentation of renewable feedstocks by microbes to produce sustainable fuels and chemicals has the potential to replace petrochemical-based production. For example, carboxylic acids produced by microbial fermentation can be used to generate primary building blocks of industrial chemicals by either enzymatic or chemical catalysis. In order to achieve the titer, yield and productivity values required for economically viable processes, the carboxylic acid-producing microbes need to be robust and well-performing. Traditional strain development methods based on mutagenesis have proven useful in the selection of desirable microbial behavior, such as robustness and carboxylic acid production. On the other hand, rationally-based metabolic engineering, like genetic manipulation for pathway design, has becoming increasingly important to this field and has been used for the production of several organic acids, such as succinic acid, malic acid and lactic acid. This review investigates recent works on Saccharomyces cerevisiae and Escherichia coli, as well as the strategies to improve tolerance towards these chemicals.

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Enhancement of Polyunsaturated Fatty Acid Production by Tn5 Transposon in Shewanella baltica

Cited by 24 publications

References 19 publications

Enhanced heterologous production of eicosapentaenoic acid in Escherichia coli cells that co-express eicosapentaenoic acid biosynthesis pfa genes and foreign DNA fragments including a high-performance catalase gene, vktA

Enhanced heterologous production of eicosapentaenoic acid in Escherichia coli cells that co-express eicosapentaenoic acid biosynthesis pfa genes and foreign DNA fragments including a high-performance catalase gene, vktA

Recombinant production of omega-3 fatty acids in Escherichia coli using a gene cluster isolated from Shewanella baltica MAC1

Metabolic Engineering of Biocatalysts for Carboxylic Acids Production

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