Enhancement of polyunsaturated fatty acid production under low-temperature stress in Cylindrotheca closterium

Almeyda, María Delfina; Bilbao, P.; Popovich, Cecilia A.; Constenla, Diana Teresita; Leonardi, Patricia I.

doi:10.1007/s10811-020-02047-x

Cited by 22 publications

(14 citation statements)

References 71 publications

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“…In general, neutral lipids are the main lipid fraction found in diatoms [7,[21][22][23]. The production of TAG by H. coffeaeformis increased with the number of days in culture in the photobioreactor, and its value (in mg·L −1 ) was comparable to that reported for another diatom from Bahía Blanca Estuary grown under similar conditions [24]. Moreover, diatom biomass is valuable in terms of its fatty acid composition, as it can contain high levels of essential poly-unsaturated long chain fatty acids (PUFAs) such as eicosapentaenoic (EPA) and arachidonic (ARA) acids [25].…”

Section: Discussionsupporting

confidence: 71%

Assessment of Halamphora coffeaeformis Growth and Biochemical Composition for Aquaculture Purposes

Bilbao

Martín

Popovich

et al. 2020

JMSE

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The vast majority of the world's fishery by-products are utilized in the fish farming industry. However, due to the high cost and unsustainability of these by-products, alternative sources must be found. Marine diatoms produce important amounts of fatty acids, sterols, proteins and carbohydrates. In this work, we assessed the nutritional value of Halamphora coffeaeformis at the exponential growth phase, to determine its suitability for aquafeed. The strain was grown in a photobioreactor at 20 • C for 6 days. The production of proteins, lipids and carbohydrates was determined, and essential fatty acid, sterol and amino acid composition was assessed. The highest values of triacylglycerides (TAG), free and esterified sterols, proteins and carbohydrates were found after 6 days of growth in the photobioreactor. Fatty acid analysis by gas chromatography showed the presence of eicosapentaenoic (EPA) and arachidonic (ARA) in amounts similar to those for fish oils. In terms of sterols, a predominance of stigmasterol was observed, followed by cholesterol. The amino acid composition revealed 50% of them to be among those essential for fish and other aquatic animals. Finally, a suitable amount of carbohydrates was found in H. coffeaeformis cultures. Together, these findings support the use of H. coffeaeformis as an alternative and sustainable source for aquafeed to partially replace the use of fishery by-products.

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Section: Discussionsupporting

confidence: 71%

Assessment of Halamphora coffeaeformis Growth and Biochemical Composition for Aquaculture Purposes

Bilbao

Martín

Popovich

et al. 2020

JMSE

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“…A two-fold increase in PUFAs with respect to indoor cultures, in which higher temperature values were constantly maintained, was detected [35]. This strategy was found to be effective also in diatom species: A drastic temperature decrease by 9 degrees (from 20 to 11 • C) stimulated PUFAs' production in Cylindrotheca closterium during the stationary phase [36]. PUFAs were also found to reach high proportions (i.e., ca.…”

Section: Temperaturementioning

confidence: 99%

Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture

et al. 2021

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Microalgae have a great potential for the production of healthy food and feed supplements. Their ability to convert carbon into high-value compounds and to be cultured in large scale without interfering with crop cultivation makes these photosynthetic microorganisms promising for the sustainable production of lipids. In particular, microalgae represent an alternative source of polyunsaturated fatty acids (PUFAs), whose consumption is related to various health benefits for humans and animals. In recent years, several strategies to improve PUFAs’ production in microalgae have been investigated. Such strategies include selecting the best performing species and strains and the optimization of culturing conditions, with special emphasis on the different cultivation systems and the effect of different abiotic factors on PUFAs’ accumulation in microalgae. Moreover, developments and results obtained through the most modern genetic and metabolic engineering techniques are described, focusing on the strategies that lead to an increased lipid production or an altered PUFAs’ profile. Additionally, we provide an overview of biotechnological applications of PUFAs derived from microalgae as safe and sustainable organisms, such as aquafeed and food ingredients, and of the main techniques (and their related issues) for PUFAs’ extraction and purification from microalgal biomass.

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“…The second process is applied with stress condition such as low temperature to stimulate the accumulation of the secondary metabolites. For instance, moving Cylindrotheca closterium from 20 • C incubator (stationary phase) to 10 • C, enhanced the accumulation of PUFA (Almeyda et al, 2020). The staged temperature control method combined with cold stress stimulation strategy which is important for fatty acids accumulation has been studied in the previous research.…”

Section: Introductionmentioning

confidence: 99%

“…( Wang et al, 2022 ), Chlorella sp. ( Zheng et al, 2021 ), Cylindrotheca closterium ( Almeyda et al, 2020 ), Isochrysis galbana ( Cao et al, 2020 ), Nannochloropsis salina ( Gill et al, 2018 ), and Scenedesmus sp. ( Calhoun et al, 2021 ) to investigate its effects on the yield of fatty acids (summarized as Supplementary Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, most of the studies focused on the one-step fermentation with low temperature (shown as Supplementary Table 1 ). It was reported that compares with one-step fermentation, the staged temperature control method is important for accumulating the total amount of lipids ( Almeyda et al, 2020 ). Staged temperature control fermentation strategy consists of two main processes with various temperatures.…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptomic and lipidomic analyses indicate that cold stress enhanced the production of the long C18–C22 polyunsaturated fatty acids in Aurantiochytrium sp.

Song

Zheng³

et al. 2022

Front. Microbiol.

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Aurantiochytrium sp. belonging to Thraustochytrids are known for their capacity to produce long-chain polyunsaturated fatty acids (PUFAs). However, effects of cold stress accompanied with staged-temperature control on the fatty acid metabolism in Aurantiochytrium sp. were rarely studied. In this study, cold stress (15°C, 5°C) was applied for Aurantiochytrium sp., with the physiological responses (morphology, growth, fatty acid profiling) and gene expression related FA synthesis, lipid metabolism, and regulatory processes was observed. Results showed that there is a significant change for the lipid types under 5°C (251 species) and 15°C (97 species) treatment. The 5°C treatment was benefit for the C18–C22 PUFAs with the yield of docosahexaenoic acid (DHA) increased to 1.25 times. After incubation at 15°C, the accumulation of eicosadienoic acid (EA) (20:2) was increased to 2.00-fold. Based on transcriptomic and qPCR analysis, an increase in genes involved in fatty acid synthase (FAS) and polyketide synthase (PKS) pathways was observed under low-temperature treatment. With upregulation of 3-ketoacyl-CoA synthase (2.44-fold), ketoreductase (2.50-fold), and dTDP-glucose 4,6-Dehydratase (rfbB) (2.31-fold) involved in PKS pathway, the accumulation of DHA was enhanced under 5°C. While, FAS and fatty elongase 3 (ELO) involved in the FAS pathway were upregulated (1.55-fold and 2.45-fold, respectively) to accumulate PUFAs at 15°C. Additionally, glycerol-3-phosphate acyltransferase (GPAT), lysophospholipid acyltransferase (LPAT), phosphatidic acid phosphatase (PAP), phosphatidylserine synthase (PSS), and phosphatidylserine decarboxylase (PSD) involved in glycerophospholipid biosynthesis were upregulated at 5°C increasing the accumulation of phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidylinositol (PI). However, glycolysis and the TCA cycle were inhibited under 5°C. This study provides a contribution to the application of two-staged temperature control in the Aurantiochytrium sp. fermentation for producing cold stress-enhancing PUFAs, in order to better understand the function of the key genes for future genetic engineering.

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Enhancement of polyunsaturated fatty acid production under low-temperature stress in Cylindrotheca closterium

Cited by 22 publications

References 71 publications

Assessment of Halamphora coffeaeformis Growth and Biochemical Composition for Aquaculture Purposes

Assessment of Halamphora coffeaeformis Growth and Biochemical Composition for Aquaculture Purposes

Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture

Comparative transcriptomic and lipidomic analyses indicate that cold stress enhanced the production of the long C18–C22 polyunsaturated fatty acids in Aurantiochytrium sp.

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