Enhancement of postoperative recovery by preoperative oral co-administration of the amino acids, cystine and theanine, in a mouse surgical model

Shibakusa, Tetsuro; Mikami, Takashi; Kurihara, Saburo; Chiba, Y.; Tsuchiya, Takashi; Miyachi, Tomohiro; Oyama, Atsushi; Tanaka, Kinji; Koyama, Naoto

doi:10.1016/j.clnu.2012.02.001

Cited by 22 publications

(21 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The findings of all the clinical trials to date are based on the combination of 700 mg cystine and 280 mg theanine; future studies should evaluate the optimal dose. In an experiment using mice, its effects were not intensified at higher doses, suggesting that there is an optimal dose [9, 17]. …”

Section: Discussionmentioning

confidence: 99%

“…The use of an anticancer agent is expected to increase oxidative stress in cells and to reduce the concentration of GSH, which is a potent antioxidant. Because the administration of cystine and theanine for 5 days before laparotomy suppressed decreases in GSH in the small bowel mucosa and Peyer’s patches of mice [9], this may be the mechanism responsible for alleviation of the AEs caused by anticancer chemotherapy. A previous study involving 14 patients who had colorectal cancer and underwent adjuvant chemotherapy with 5-FU and oxaliplatin reported that the incidence of sensory neuropathy was significantly lower in 5 patients who were orally administered N-acetylcysteine, a GSH precursor, at 1200 mg than in the 9 patients who were not administered the supplement [18].…”

Section: Discussionmentioning

confidence: 99%

“…We previously described that the intake of cystine and theanine for 10 days during the perioperative period led to the early resolution of high postoperative levels of interleukin (IL)-6 and C-reactive protein (CRP) and early recovery from changes in neutrophil and lymphocyte counts [8]. Cystine and theanine has also been shown to produce similar effects in mouse digestive tract surgery models and to prevent decreases in intestinal GSH; these effects were considered to be partly explained by the supply of GSH [9]. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Oral administration of the amino acids cystine and theanine attenuates the adverse events of S-1 adjuvant chemotherapy in gastrointestinal cancer patients

et al. 2016

Self Cite

View full text Add to dashboard Cite

BackgroundNutritional therapy is used to reduce the adverse events (AEs) of anticancer drugs. Here, we determined whether the amino acids cystine and theanine, which provide substrates for glutathione, attenuated the AEs of S-1 adjuvant chemotherapy.MethodsPatients scheduled to receive S-1 adjuvant chemotherapy were randomized to the C/T or the control groups. The C/T group received 700 mg cystine and 280 mg theanine orally 1 week before the administration of S-1, which then continued for 5 weeks. Each group received S-1 for 4 weeks. Blood sampling was performed and AEs were evaluated (CTCAE ver. 4.0) before and after the administration of S-1. S-1 was discontinued when AEs ≥ grade 2 occurred.ResultsThe incidences of AEs of any grade and those over grade 2 were lower in the C/T group than in the controls. The incidence of diarrhea (G ≥ 2) was significantly less (p < 0.05) in the C/T group (3.1 %) than in the controls (25.8 %). The duration and completion rate of the S-1 adjuvant chemotherapy were significantly longer (p < 0.01) and higher (p < 0.01), respectively, in the C/T group (complete ratio: 75.0 %, duration: 24.8 ± 5.8 days) than in the controls (complete ratio: 35.5 %, duration: 20.0 ± 7.7 days).ConclusionsThe oral administration of cystine and theanine attenuated the AEs of S-1 adjuvant chemotherapy and increased the S-1 completion rate, suggesting that cystine and theanine is a useful supportive care for chemotherapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oral administration of the amino acids cystine and theanine attenuates the adverse events of S-1 adjuvant chemotherapy in gastrointestinal cancer patients

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Indeed, CT pretreatment reportedly resulted in the maintenance of gut GSH levels in a murine intestinal manipulation model [18]. Hepatic GSH levels after intraperitoneal immunization were demonstrated to be higher in CT-treated animals than in controls [19].…”

Section: Introductionmentioning

confidence: 99%

“…The CT140 and 280 groups were given cystine (Ajinomoto Co., Inc., Tokyo, Japan) and theanine (Taiyokagaku Co., Ltd., Yokkaichi, Japan) at dosages of 140 and 280 mg/kg (cystine: theanine = 5: 2), respectively, once daily via gavage for 5 days, while the Vehicle mice received only the vehicle (0.5% methylcellulose solution). We referred to previous reports using mice for determining the CT doses [18]. Then, we performed a pilot study, wherein the mice were given 0, 70, 140, or 280 mg/kg of CT before gut ischemia reperfusion.…”

Section: Experimental Protocol Experimentsmentioning

confidence: 99%

Cystine and Theanine Improve Survival after Gut Ischemia-Reperfusion

Miyakuni

Fukatsu

et al. 2018

Ann Nutr Metab

Self Cite

View full text Add to dashboard Cite

Background and Aim: Oral administration of cystine and theanine (CT) may modulate antioxidant glutathione (GSH) metabolism, thereby improving outcomes after gut ischemia reperfusion. Methods: Experiment 1: Institute of Cancer Research mice (n = 35) were assigned to a Vehicle (n = 11), a CT140 (n = 14), or a CT280 (n = 10) group. The CT140 and 280 groups were given CT at respective dosages of 140 and 280 mg/kg (cystine: theanine = 5: 2) once daily via gavage for 5 days. All mice underwent 75-min occlusion of the superior mesenteric artery (SMA). Survival after reperfusion was observed. Experiment 2: Mice (n = 67) were pretreated for 5 days (Vehicle: n = 24, CT280: n = 20, vehicle/sham: n = 23). The Vehicle and CT280 groups underwent 60-min SMA occlusion. Levels of GSH, the oxidized form of GSH, Glutathione-S-S-Glutathione (GSSG), and GSH-related amino acids (cysteine and glutamic acid) in the small intestine, and plasma cytokine (IL-6, IL-1β, TNFα) levels, were evaluated before (0 h), 3, 6, or 9 h after reperfusion. Results: Experiment 1: The CT280 group showed significantly better survival than the Vehicle group. Experiment 2: Gut GSSG, cysteine, and glutamic acid levels were higher in the CT280 than in the Vehicle group after reperfusion. Plasma IL-6 and TNFα levels rose more rapidly in the CT280 than in the Vehicle group. Conclusion: Oral administration of CT improves survival after gut I/R, possibly through the modulation of the GSH-redox cycle and cytokine responses.

show abstract

The beneficial health effects of green tea amino acid l‐theanine in animal models: Promises and prospects for human trials

Williams

Sergi

McKune

et al. 2019

Phytotherapy Research

View full text Add to dashboard Cite

l‐Theanine (l‐THE) is a nonproteinogenic amino acid derived from green tea (Camellia sinensis), which exhibits strong antioxidant‐like properties and contributes to the favourable umami taste sensation. Several studies have reported that the consumption of this amino acid has many therapeutic effects, including improvements in brain and gastrointestinal function, cancer drug therapeutic efficacies, antihypertensive effects, and improved immune function. Considering the recent Western commercialisation and popularity of green tea consumption as a nootropic agent in humans, the aims of this review were to consolidate the existing knowledge from ex vivo and in vitro animal models and attempt to highlight the applicability of l‐THE towards the human clinical trials. Considering the anti‐inflammatory and antioxidants effects of l‐THE presented in the current review, further research must translate the existing knowledge gained from animal and cell models to exploring the potential metabolic health benefits and moderating effects on the pathogenesis of conditions such as obesity, arthritis, depression, and type 2 diabetes in human trials. This will bridge the gap in literature and provide more insights into the mechanisms driving pathologies characterised by the inflammatory response and oxidative stress.

show abstract

Enhancement of postoperative recovery by preoperative oral co-administration of the amino acids, cystine and theanine, in a mouse surgical model

Cited by 22 publications

References 28 publications

Oral administration of the amino acids cystine and theanine attenuates the adverse events of S-1 adjuvant chemotherapy in gastrointestinal cancer patients

Oral administration of the amino acids cystine and theanine attenuates the adverse events of S-1 adjuvant chemotherapy in gastrointestinal cancer patients

Cystine and Theanine Improve Survival after Gut Ischemia-Reperfusion

The beneficial health effects of green tea amino acid l‐theanine in animal models: Promises and prospects for human trials

Contact Info

Product

Resources

About