Enhancement of production and regeneration of embryogenic type II callus in Zea mays L. by AgNO3

Vain, Philippe; Yean, H.; Flament, Pascal

doi:10.1007/bf00047740

Cited by 77 publications

(34 citation statements)

References 22 publications

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“…Indeed, the use of a reduced form of exogenous nitrogen (ammonium or amino acids) is generally recommended for dispersing Oryza sativa L. cells in liquid medium (Toriyama et al 1986). Less commonly used culture medium compounds such as silver nitrate (Vain et al 1989), or modification of the calcium/cation ratio (Yoshida & Watanabe 1971) have also been found to modify callus texture in Z. mays and Nicotiana glutinosa L. respectively.…”

Section: Introductionmentioning

confidence: 97%

Callus friability and somatic embryogenesis inHevea brasiliensis

Montoro

Etienne

Michaux-Ferrière

et al. 1993

Plant Cell Tiss Organ Cult

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The influence of plant growth regulators, sucrose, calcium and various macronutrient media on callus friability and somatic embryogenesis was investigated in Hevea brasiliensis M/ill. Arg. Friable and embryogenic calli were spontaneously formed in two rubber tree clones (PR 107 and RRIM 600) on the Medium for Hevea (MH), with 3,4-dichlorophenoxyacetic acid (3,4-D), kinetin and sucrose, while compact embryogenic calli were enhanced in three other clones (PB 260, PB 235 and GT1). Callus friability was enhanced in clone PB 260 when the concentration of one growth factor (3,4-D or kinetin) was reduced from 4.5 IxM to 0.45 IxM during the first culture, or when high sucrose or calcium levels 351 mM and 12mM, respectively) were maintained during subcultures. The different macronutrient media did not alter callus texture but only use of MH and Murashige and Skoog (MS) media led to somatic embryogenesis. Friable calli obtained by modifying the auxin/cytokinin balance lost their embryogenic potential. In contrast, those obtained on media with high sucrose or calcium concentrations were mainly composed of embryogenic cells embedded in a mucilaginous matrix. Such calli could be of potential interest for establishing embryogenic cell suspension cultures.Abbreviations: BC-brown calli, 3,4-D-3,4-dichlorophenoxyacetic acid, EA-embryogenic activity, EC-callus bearing embryos, FC-friable calli, FEC-friable callus bearing embryos, MA-meristematic activity, MH-Medium for Hevea, Muc-mucilage, OP-oxidized polyphenols

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Section: Introductionmentioning

confidence: 97%

Callus friability and somatic embryogenesis inHevea brasiliensis

Montoro

Etienne

Michaux-Ferrière

et al. 1993

Plant Cell Tiss Organ Cult

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“…Improvements such as incorporation of silver nitrate, as was done for Zea mays L. (VAIN et al 1989) in order to reduce the deviation that could be caused by unknown environmental factors, may reveal the full scope of such differences. In the present studies, an inhibition by ethylene for some tef cultivars seems to have occurred, judging by the brownish colour observed.…”

Section: Resultsmentioning

confidence: 99%

Somatic Embryogenesis and Plant Regeneration from Leaf and Root Explants and from Seeds of Eragrostis tef (Gramineae)

Bekele¹,

Klöck²,

Zimmermann³

2004

Hereditas

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In vitro somatic embryogenesis and plant regeneration from root and leaf explants and from seeds were studied using eight genotypes of Eragrostis tef at various concentrations (1–10 mg/l) of 3,6‐dichloromethoxybenzoic acid (3,6‐D) and 2,4‐dichloromethoxybenzoicacid (2,4‐D). Optimal conditions for callus growth, embryogenesis and plant regeneration were evaluated. The callus biomass and the ability to regenerate plants were found to be functions of type of explant and hormone concentration. The callus fresh weight from leaf was higher than that from root, and different genotypes differed in their callus fresh weight. Unlike leaf induced callus, the root induced callus showed no significant differences between genotypes and treatments. The fresh weight of callus from seeds of most genotypes studied was similar. The number of direct regenerants from callus maintained on callus induction medium varied among the genotypes studied. On the whole, the number of regenerants from leaf callus was higher than that from root callus at all tested hormone concentrations. 1 and 2.5 mg/l of 3, 6‐D gave the highest numbers of regenerants from calli transferred to embryogenesis medium. The various tef genotypes differed in their capacity to regenerate plants. Establishment of cell suspension cultures from root and leaf explants was also accomplished.

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“…Different kinds of plant growth regulators are needed in different stage of bamboo regeneration, and auxin and cytokinin are the critical components for the morphogenesis in vitro. Besides, ethylene, abscisic acid (ABA) and brassinosteroid (BR) will also have different effects on embryogenesis and organogenesis (Aydin et al, 2006;Torrizo & Zapata, 1986;Vain et al, 1989).…”

Section: Selection Of Mediamentioning

confidence: 99%

“…The silver ion is reported to be an inhibitor of ethylene action by competitively binding to ethylene receptors which are located predominantly at the intracellular membrane, while AVG inhibits ethylene biosynthesis directly (Vain et al, 1989;Zhang et al, 1998). In addition to silver ion, other heavy metals including Cu and other ethylene inhibitors (Co and Ni), also significantly facilitate the regeneration and somatic embryogenesis (Purnhauser & Gyulai, 1993;Roustan et al 1989).…”

Section: Chemical Additivesmentioning

confidence: 99%