Enhancement of radiation effects by mercury in preimplantation mouse embryos in vitro

Müller, Wolfgang‐Ulrich; Streffer, C.; Fischer-Lahdo, Christine

doi:10.1007/bf00343120

Cited by 18 publications

(5 citation statements)

References 13 publications

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“…Beside this, cadmium binds with thiol group of cellular components, which are mainly responsible for protecting repair system against damage caused by radiation-induced free radicals [13]. Due to indirect effects of irradiation, free radicals are formed which are scavenged by -SH group, an inherent protective mechanism present in the cells.…”

Section: Results and Discussion:-mentioning

confidence: 99%

Induction of Biochemical Changes in Mouse Testis After the Combined Treatment of Radiation and Cadmium Chloride.

Verma¹,

Rk²,

Gupta³

2017

IJAR

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Section: Results and Discussion:-mentioning

confidence: 99%

Induction of Biochemical Changes in Mouse Testis After the Combined Treatment of Radiation and Cadmium Chloride.

Verma¹,

Rk²,

Gupta³

2017

IJAR

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“…In combined exposure cadmium interacts with ionizing radiation because of its high affinity for -SH and disulphide groups of proteins 16 . In addition to this, Cadmium combines with Thiol groups of cellular components, which in turn, are primarily responsible for protecting repair systems against damage caused by radiation-induced free radicals 17 . Indirect effects of radiations causes formation of free radicals which are scavenged by -SH groups, an inherent protective mechanism present in the cells.…”

Section: Discussionmentioning

confidence: 99%

Histopathological Changes in the Spleen of Swiss albino Mice after the Combined Exposure of Radiation and Cadmium

Daverey¹

2020

Asian J Pharm Res Dev

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With the technological advancement and diversification of industries, combined with specialization in all fields, the volume and complexity of metals is also increasing day by day. Interaction of metals with other agents is an important aspect as both can interact in a “synergistic” or “additive synergistic” manner, further aggravating the situation. In the present study, combined effect of radiation and cadmium on spleen of mice has been investigated. For the experiment, adult, healthy male Swiss albino mice were exposed to different doses of radiation and also fed with the aqueous solution of CdCl2 which was prepared by dissolving 20 mg of cadmium chloride in 1000 ml of glass distilled water, thus giving a concentration of 20 ppm and then administered orally ad libitum in drinking water continuously, till the end of the experiment. Animals were autopsied by cervical dislocation at each post- interval of 1, 2, 4, 7, 10, 14 and 28 days. Spleen was taken out, weighed, fixed in Bouin’s fluid, dehydrated and embedded in paraffin wax. Transverse sections were cut at 5µ from middle part of the tissue and stained with Harri’s haematoxylin-eosin stain for histopathological studies. Pathological changes after combined exposure in the present investigation depends upon the total dose of radiation provided i.e. higher the dose, higher the damage. Most striking histopathological change in the spleen was loss of lymphoid structure, inflammation, fibrous tissue proliferation, pyknosis, necrosis, karyolysis, karyorrhexis.

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“…Quail development was similarly found to be unaffected by prolonged exposure (up to 16 days) to even very powerful static magnetic fields (16). Interestingly, the murine embryo model has previously been used to evaluate the effects of other imaging modalities, especially that of X‐ray irradiation (17–22). For example, Molls and Streffer (17) studied the effects of X‐ray irradiation on two‐cell murine embryos in 1984, and reported impaired development of blastocysts in the exposed group.…”

Section: Discussionmentioning

confidence: 99%

“…For example, Molls and Streffer (17) studied the effects of X‐ray irradiation on two‐cell murine embryos in 1984, and reported impaired development of blastocysts in the exposed group. Murine embryos have also been used to study the effects of X‐ray irradiation in combination with mercuric chloride (18), lead (19), caffeine (20), and tributyl phosphate (21). One advantage of using the murine embryo model is that the complete organism is being studied instead of single cells.…”

Section: Discussionmentioning

confidence: 99%

The effects of 1.5T magnetic resonance imaging on early murine in‐vitro embryo development

Chew

Ahmadi

Goh

et al. 2001

Magnetic Resonance Imaging

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Although no ionizing radiation is involved, patients undergoing magnetic resonance imaging (MRI) are exposed to powerful static magnetic fields, magnetic field gradients, and radio-frequency fields that may be potentially damaging. Our study aims to document the effect of MRI imaging sequences on early murine embryo development (two-cell to blastocyst stage) in vitro. Two-cell murine embryos were exposed to various lengths of MRI using pulse sequences employed in present day clinical imaging. Early murine embryo development was documented in vitro, and blastocyst development rates were computed for both the control and exposed groups. There were no significant differences detected in the rate of blastocyst formation between the control groups and the embryos exposed to MRI. J. Magn. Index terms: MRI; murine blastocyst; in-vitro development; safety; embryogenesis ALTHOUGH NO IONIZING RADIATION is involved, patients undergoing MRI are still exposed to powerful static magnetic fields, magnetic field gradients, and radio frequency (RF) fields that may potentially be damaging. While a few authors (1-3) have studied the effects of MRI on pregnant mice in mid-gestation, no work has been done to study the effect of pulse sequences utilized for present-day routine NMR scanning on early embryo development. Our study aims to document the effect of modern MR Imaging sequences on early murine embryo development (two-cell to blastocyst stage) in vitro. MATERIALS AND METHODSNewly arrived three-to four-week-old female CBA/C57 Fl mice were allowed to acclimatize for one week in a windowless room at the University's Animal Holding Unit. The males were kept individually in separate cages. Lighting was controlled by an automated timer that provided 12 hours of light and 12 hours of darkness. The temperature and relative humidity were maintained at 22°C and 70%. Water and food pellets were provided ad libidum.The female mice were superovulated with intraperitoneal injections of 10 IU PMSG (Folligon; Intervet, Boxmeer, Holland), and 48 hours later 10 IU of hCG (Chorulon; Intervet, Boxmeer, Holland). The females were then mated with proven-fertile Swiss Albino. Mating would usually occur around midnight, and the females would be checked the following morning for the presence of vaginal plugs, which indicated successful mating.The successfully mated females were then killed by cervical dislocation 36 -40 hours post hCG injection. The mice were dissected and both the oviducts were recovered and washed in T6 ϩ 4BSA. Under a stereo dissecting microscope, clusters of two-cell embryos were seen in the ampullary region of the oviduct. The oviduct was then teased with a pair of 21G, 1 1 2 -inch hypodermic needles in fresh medium. The embryos obtained were then washed 4 times in 40 -60 L of T6 ϩ 4BSA under paraffin oil. These two-cell embryos were transferred to a fresh dish containing about 100 L of T6 ϩ 4BSA under paraffin oil for culture and incubated at 37°C in a humidified atmosphere of 5% CO 2 in air.Embryos were then transported from th...

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Enhancement of radiation effects by mercury in preimplantation mouse embryos in vitro

Cited by 18 publications

References 13 publications

Induction of Biochemical Changes in Mouse Testis After the Combined Treatment of Radiation and Cadmium Chloride.

Induction of Biochemical Changes in Mouse Testis After the Combined Treatment of Radiation and Cadmium Chloride.

Histopathological Changes in the Spleen of Swiss albino Mice after the Combined Exposure of Radiation and Cadmium

The effects of 1.5T magnetic resonance imaging on early murine in‐vitro embryo development

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