Enhancement of recombinant cholera toxin B subunit production in Escherichia coli by applying a fed-batch control strategy

Mendoza-Vega, O.; Buri, E.; Speck, Denis

doi:10.1007/bf00143096

Cited by 6 publications

(1 citation statement)

References 10 publications

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“…This vaccine also protects against diarrhea caused by the heatlabel enterotoxin of Escherichia coli [2]. It is also possible to produce the CTB by cultivating E. coli [3], even though the protein then will accumulate in the intracellular fraction. Another way to produce cholera vaccine is to use a live and attenuated strain as described in [4] and [5].…”

Section: Introductionmentioning

confidence: 99%

Probing control of glucose feeding in Vibrio cholerae cultivations

Maré

Andersson²,

Hagander

2003

Bioprocess Biosyst Eng

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Infection with Vibrio cholerae is a significant problem in many developing countries. Cultivation of V. cholerae is used in production of cholera toxin B subunit, which is a component in a cholera vaccine. Fed-batch cultivations with V. cholerae in defined media have been conducted and reproducible results were obtained. A probing feeding strategy developed by Akesson for Escherichia coli cultivations has been tested. The strategy is working as well for V. cholerae as for E. coli in minimizing the amount of acetic acid formed and avoiding anaerobic conditions. At 2 h after the feed start most of the acetic acid accumulated during the batch phase is consumed. The resulting feed rate tends to be the highest possible with respect to the constraints from cell metabolism and mass transfer, thus maximizing productivity in terms of biomass. A cell dry weight of 20-23 g/l is obtained after 12 h of feeding.

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Section: Introductionmentioning

confidence: 99%