Enhancement of reproductive performances of Gangetic leaffish, Nandus nandus through up regulation of serum Ca2+ concentration, improved morphological alteration of liver and ovary with dietary polyunsaturated fatty acids

Abstract

Incorporation of polyunsaturated fatty acids (PUFAs) as biofunctional compounds with feed is an effective way for gonadal maturation without any hazardous effects on animal health, and thus it is possible to save the vulnerable species from the danger of extinction. In the present study sperm quality, level of Ca(2+) concentration in serum, histological structure of the liver and developmental stages of ovary of an endangered fish species, Nandus nandus were investigated for the confirmation of the positive effects of PUFAs in reproduction and gonadal maturation. Fishes were collected from Brahmaputra River, Mymensingh, Bangladesh. Treated group was fed 1% squid extracted phospholipid supplemented diet that was mixed with silver carp fish muscle where as controlled group was fed the same except phospholipid. For histology of liver and gonads, samples were dehydrated, cleaned and infiltrated, embedded in paraffin wax and sectioned. After that, the samples were stained with hematoxylin and eosin. The photomicrographs of the stained samples were taken by using light microscope. In comparison with the control group, treated group exhibited higher gonadal maturation which resulted in spontaneous spawning. Treated female demonstrated advanced gonadal developmental stages in comparison with the controlled female during different months. During spawning season, lipid granules and normal morphological alteration were observed in case of treated fish liver, whereas less lipid granules with more histological alteration of liver were observed in control group. Serum Ca(2+) concentration in treated female was found significantly higher (P < 0.01) in contrast to the controlled female during the breeding season which was an indicator of the augment of estrogen secretion during ovarian maturation. Better sperm quality, early maturation of oocytes, less histological alteration of liver hepatocytes and spontaneous spawning performances of PUFA-treated fish were as a result of the efficiency of PUFAs in enhancing maturation. The experiment suggests that supplementation of dietary PUFAs improve the spawning performances of fish.

Spawning and developmental biology of endangered Pabdah catfish, Ompok pabda (Hamilton, 1822)

“…Motin et al (2013) have found the highest GSI (%) 4.06 in September and 0.76 in December for female and male of O. pabo, respectively. Similar GSI (%) has also been reported in Colisa fasciatus and Nandus nandus (Hossen et al, 2014b;Reza et al, 2013). The values of correlation coefficient (r) were established between male GSI (%) and female GSI (%) and amongst the body weight and gonad weight and fecundity.…”

Arachidonic Acid in the Diet of Captive Yellowtail and Its Effects on Egg Quality

“…; Reza et al. ). The intake of LC‐PUFAs from the diet is the only way for marine fish to obtain n‐3 and n‐6 essential FAs, such as EPA, DHA, and ARA, since most marine fish studied to date have shown a limited ability to synthesize these FAs from their precursors, linolenic acid and linoleic acid (Bell et al.…”

Effects of dietary polyunsaturated fatty acid on reproduction and developmental biology of Gangetic Mystus (Mystus cavasius) (Hamilton-Buchanan, 1822)

“…A positive correlation was detected between the levels of n-3 HUFA in the diet and eggs with the EPA concentration being more readily affected by dietary n-3 HUFA than DHA. Selective maintenance of DHA has also been seen during embryogenesis (Izquierdo et al, 2001) and denoting the essentiality of this fatty acid for the development of embryo and larvae (Reza et al, 2013). The percentage of morphologically normal eggs (as a parameter to determine egg viability) has been found to increase with an increase in the n-3 PUFA content in broodstock diets and an inclusion of these fatty acids as lipid droplets into the eggs (Reza et al, 2013;Hossen et al, 2014b), thus showing the importance of essential fatty acids for normal development of eggs and embryo.…”